Voltammetric measurement of reduced nicotinamide-adenine nucleotides and application to amperometric measurement of enzyme reactions.

Abstract The electrochemical oxidations of reduced nicotinamide-adenine dinucleotide and reduced nicotinamide-adenine dinucleotide phosphate on platinum and carbon electrodes are described. Well defined voltammetric anodic waves are observed on carbon electrodes, with a linear relationship between peak height and concentration for 0–0.5m M NADH and NADPH. Amperometric methods for NAD oxidoreductase analyses by direct electrochemical oxidation of the reduced nucleotide have been developed for lactic dehydrogenase and ethanol in serum.