KPC-2 and OXA-48 carbapenemase-harbouring Enterobacteriaceae detected in an Austrian wastewater treatment plant.

Multiresistant Enterobacteriaceae, like carbapenemase-producing strains, have their primary reservoir in medical institutions. They can also be found with increasing tendency in other reservoirs. One possible way for entrance of multiresistant Enterobacteriaceae into the environment is via waste water. The aim of the study was to screen isolates from a wastewater treatment plant for the presence of carbapenemase-producing Enterobacteriaceae. Three isolates harboured carbapenemase genes, one Klebsiella pneumoniae harboured KPC-2 and one K. pneumoniae and one Escherichia coli harboured OXA-48. This is the first report of carbapenemase-harbouring Enterobacteriaceae isolated outside medical institutions in Austria and the first detection of KPC-harbouring K. pneumonia MLST ST 1245.

[1]  E. Leitner,et al.  Resistance patterns of Escherichia coli isolated from sewage sludge in comparison with those isolated from human patients in 2000 and 2009. , 2013, Journal of water and health.

[2]  R. Stephan,et al.  Characteristics of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Isolates from Rivers and Lakes in Switzerland , 2013, Applied and Environmental Microbiology.

[3]  E. Leitner,et al.  Comparison of extended-spectrum-β-lactamase (ESBL) carrying Escherichia coli from sewage sludge and human urinary tract infection. , 2013, Environmental pollution.

[4]  M. Hoenigl,et al.  Ventilator-associated pneumonia caused by OXA-48-producing Escherichia coli complicated by ciprofloxacin-associated rhabdomyolysis , 2013, Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy.

[5]  T. C. Ngo,et al.  blaNDM-1–positive Klebsiella pneumoniae from Environment, Vietnam , 2012, Emerging infectious diseases.

[6]  L. Wieler,et al.  Extended-spectrum b-lactamase-producing and AmpC-producing Escherichia coli from livestock and companion animals , and their putative impact on public health : a global perspective , 2012 .

[7]  V. Miriagou,et al.  Rapid evolution and spread of carbapenemases among Enterobacteriaceae in Europe. , 2012, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[8]  P. Nordmann,et al.  Carbapenem resistance in Enterobacteriaceae: here is the storm! , 2012, Trends in molecular medicine.

[9]  D. Orth,et al.  Emergence of VIM-1-carbapenemase-producing Enterobacter cloacae in Tyrol, Austria. , 2012, Journal of medical microbiology.

[10]  E. Leitner,et al.  Nosocomial Outbreak of Klebsiella pneumoniae Carbapenemase-Producing Klebsiella oxytoca in Austria , 2012, Antimicrobial Agents and Chemotherapy.

[11]  P. Nordmann,et al.  Environmental KPC-Producing Escherichia coli Isolates in Portugal , 2011, Antimicrobial Agents and Chemotherapy.

[12]  E. Leitner,et al.  Emergence of carbapenem-resistant Enterobacteriaceae in Austria, 2001-2010. , 2011, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[13]  Thierry Naas,et al.  Global Spread of Carbapenemase-producing Enterobacteriaceae , 2011, Emerging infectious diseases.

[14]  P. Nordmann,et al.  Characterization of OXA-181, a Carbapenem-Hydrolyzing Class D β-Lactamase from Klebsiella pneumoniae , 2011, Antimicrobial Agents and Chemotherapy.

[15]  T. P. G. Chagas,et al.  Occurrence of KPC-2-producing Klebsiella pneumoniae strains in hospital wastewater. , 2011, The Journal of hospital infection.

[16]  K. Jankowska,et al.  Antimicrobial resistance of fecal indicators in municipal wastewater treatment plant. , 2010, Water research.

[17]  Hong-Wei Zhou,et al.  High Diversity of Extended-Spectrum Beta-Lactamase-Producing Bacteria in an Urban River Sediment Habitat , 2010, Applied and Environmental Microbiology.

[18]  E. Leitner,et al.  ESBL-producing E. coli in Austrian sewage sludge. , 2010, Water research.

[19]  A. Carattoli.  Animal reservoirs for extended spectrum β-lactamase producers , 2008 .

[20]  A. Carattoli.  Animal reservoirs for extended spectrum beta-lactamase producers. , 2008, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[21]  N. Woodford,et al.  CTX-M: changing the face of ESBLs in Europe. , 2006, The Journal of antimicrobial chemotherapy.

[22]  Daniel Falush,et al.  Sex and virulence in Escherichia coli: an evolutionary perspective , 2006, Molecular microbiology.

[23]  Sylvain Brisse,et al.  Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates , 2005, Journal of Clinical Microbiology.

[24]  P. Bradford,et al.  Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 beta-lactamases in New York City. , 2004, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[25]  P. Nordmann,et al.  Emerging carbapenemases in Gram-negative aerobes. , 2002, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.