Detection of SNPs in the BMP6 Gene and Their Association with Carcass and Bone Traits in Chicken

BMP6, a member of the subfamilies of the morphogenetic proteins (BMPs), plays a crucial role in osteogenic and chondrocyte differentiation in vitro and stimulates chondrogenesis, making chondrocytes differentiate on their terminal stage. The objective of this study is to explore the relationship between polymorphism of BMP6 gene and slaughter traits in chicken respectively. We screened the exonic and intronic regions of BMP6 gene by DNA pool construction and amplified DNA fragment by PCR, and finally, we got nine SNPs. Association analysis revealed that BMP6 had no significant association among all slaughter traits in Yellow bantam chicken. However, BMP6 had a significant difference with femur weight, tibia weight, femur length (p<0.05), and was extremely significant with tibia length (p<0.01) in Avian chicken. Moreover, femur perimeter also had significant correlation with BMP6 in Avian chicken. These results provide useful information for further investigation on the function of chicken BMP6 gene.

[1]  C. Edwards,et al.  The Role of the Microenvironment in Prostate Cancer-Associated Bone Disease , 2016, Current Osteoporosis Reports.

[2]  C. Beaumont,et al.  Heterozygous Mutations in BMP6 Pro-peptide Lead to Inappropriate Hepcidin Synthesis and Moderate Iron Overload in Humans. , 2016, Gastroenterology.

[3]  C. Enns,et al.  Bone Morphogenetic Protein-6 Mutations Take Their Place in Iron Overload Diseases. , 2016, Gastroenterology.

[4]  Yan Wang,et al.  MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum , 2015, BioMed research international.

[5]  Heebal Kim,et al.  Genome-wide Association Study of Integrated Meat Quality-related Traits of the Duroc Pig Breed , 2014, Asian-Australasian journal of animal sciences.

[6]  S. Vukicevic,et al.  Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity , 2013, International Orthopaedics.

[7]  M. Goddard,et al.  A genome-wide association study of meat and carcass traits in Australian cattle. , 2011, Journal of animal science.

[8]  Paolo Ventura,et al.  Serum and liver iron differently regulate the bone morphogenetic protein 6 (BMP6)‐SMAD signaling pathway in mice , 2011, Hepatology.

[9]  K. Sugiura,et al.  Does Bone Morphogenetic Protein 6 (BMP6) Affect Female Fertility in the Mouse?1 , 2010, Biology of reproduction.

[10]  S. Thein,et al.  Association of sickle avascular necrosis with bone morphogenic protein 6 , 2009, Annals of Hematology.

[11]  C. Camaschella.  BMP6 orchestrates iron metabolism , 2009, Nature Genetics.

[12]  M. Roth,et al.  Lack of the bone morphogenetic protein BMP6 induces massive iron overload , 2009, Nature Genetics.

[13]  Yin Xia,et al.  BMP6 is a key endogenous regulator of hepcidin expression and iron metabolism , 2009, Nature Genetics.

[14]  R. Davoli,et al.  Investigation of candidate genes for glycolytic potential of porcine skeletal muscle: Association with meat quality and production traits in Italian Large White pigs. , 2008, Meat science.

[15]  D. Kang,et al.  Single-Nucleotide Polymorphisms and Haplotypes of Bone Morphogenetic Protein Genes and Peripheral Bone Mineral Density in Young Korean Men and Women , 2006, Calcified Tissue International.

[16]  Kozo Nakamura,et al.  Involvement of Endogenous Bone Morphogenetic Protein (BMP) 2 and BMP6 in Bone Formation* , 2005, Journal of Biological Chemistry.

[17]  P. Sebastiani,et al.  Association of klotho, bone morphogenic protein 6, and annexin A2 polymorphisms with sickle cell osteonecrosis. , 2005, Blood.

[18]  C. Maltin,et al.  Slow fiber cluster pattern in pig longissimus thoracis muscle: implications for myogenesis. , 2003, Journal of animal science.

[19]  H. Huang,et al.  A novel role for bone morphogenetic proteins in the synthesis of follicle-stimulating hormone. , 2001, Endocrinology.

[20]  T. Ochi,et al.  Mechanical Tension‐Stress Induces Expression of Bone Morphogenetic Protein (BMP)‐2 and BMP‐4, but Not BMP‐6, BMP‐7, and GDF‐5 mRNA, During Distraction Osteogenesis , 1999, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[21]  C. Grimsrud,et al.  BMP‐6 Is an Autocrine Stimulator of Chondrocyte Differentiation , 1999, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[22]  M. Mehler,et al.  Bone morphogenetic proteins in the nervous system , 1997, Trends in Neurosciences.

[23]  V. Rosen,et al.  Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[24]  B. Hogan,et al.  Patterns of expression of murine Vgr-1 and BMP-2a RNA suggest that transforming growth factor-beta-like genes coordinately regulate aspects of embryonic development. , 1989, Genes & development.

[25]  D. L. Weeks,et al.  A maternal mRNA localized to the vegetal hemisphere in xenopus eggs codes for a growth factor related to TGF-β , 1987, Cell.

[26]  M. Urist,et al.  Bone: Formation by Autoinduction , 1965, Science.

[27]  A. Gambero,et al.  Mice that are fed a high-fat diet display increased hepcidin expression in adipose tissue. , 2013, Journal of nutritional science and vitaminology.

[28]  B. Hogan,et al.  Mice lacking Bmp6 function. , 1998, Developmental genetics.

[29]  J. Garnier,et al.  The role of major genes and DNA technology in selection for meat quality in pigs. , 1998, Meat science.