IN VIVO INDUCTION OF 4‐THIOURIDINE‐CYTIDINE ADDUCTS IN tRNA OF E. COLI B/r BY NEAR‐ULTRAVIOLET RADIATION *

Abstract— Near‐ultraviolet (near‐UV; 320–405 nm) irradiation of Escherichia coli B/r induces the formation in vivo of 4Srd‐Cyd adducts in transfer RNA, as evidenced by (1) fluorescence spectrum changes of tRNA extracted from irradiated cells and reduced with NaBH4, (2) thin‐layer chromatography on cellulose of hydrolysates of trichloroacetic acid‐precipitable extracts of irradiated cells, and (3) comparison of these findings with adduct formation induced by near‐UV irradiation of purified mixed tRNA from E. coli.

[1]  T. V. Ramabhadran.  EFFECTS OF NEAR‐ULTRAVIOLET and VIOLET RADIATIONS (313–405 NM) ON DNA, RNA, and PROTEIN SYNTHESIS IN E. COLI B/r: IMPLICATIONS FOR GROWTH DELAY * , 1975, Photochemistry and photobiology.

[2]  A. Favre,et al.  Conformation and functioning of tRNAs: cross-linked tRNAs as substrate for tRNA nucleotidyl-transferase and aminoacyl synthetases. , 1974, Biochimie.

[3]  P. Dennis,et al.  Macromolecular Composition During Steady-State Growth of Escherichia coli B/r , 1974, Journal of bacteriology.

[4]  J. Ofengand,et al.  Use of photochemically induced cross-linking as a conformational probe of the tertiary structure of certain regions in transfer ribonucleic acid. , 1973, Biochemistry.

[5]  H. Bremer,et al.  Regulation of ribonucleic acid synthesis in Escherichia coli B-r: an analysis of a shift-up. II. Fraction of RNA polymerase engaged in the synthesis of stable RNA at different steady-state growth rates. , 1973, Journal of molecular biology.

[6]  R. Tyrrell,et al.  INDUCTION OF PYRIMIDINE DIMERS IN BACTERIAL DNA BY 365 nm RADIATION * , 1973, Photochemistry and photobiology.

[7]  D. Bergstrom,et al.  Structure of the borohydride reduction product of photolinked 4-thiouracil and cytosine. Fluorescent probe of transfer ribonucleic acid tertiary structure. , 1972, Journal of the American Chemical Society.

[8]  B. Roques,et al.  Chemical structures of the TU‐C and TU‐Cred products derived from E. coli tRNA , 1972, FEBS letters.

[9]  J. Jagger,et al.  Induction by cyanide, dinitrophenol, and trinitrophenol of growth inhibition and of recovery from far-ultraviolet killing in Escherichia coli B: relationship to photoprotection. , 1972, Radiation research.

[10]  A. Favre,et al.  Introduction of an intramolecular flourescent pobe in E. coli tRNAVal 1 , 1971, FEBS letters.

[11]  D. Bergstrom,et al.  Photoproducts from 4-thiouracil and cytosine and from 4-thiouridine and cytidine: refinement of tertiary tRNA structure. , 1971, Biochemical and biophysical research communications.

[12]  F. Gros,et al.  Biological activity of irradiated tRNA Val containing a 4-thiouridine-cytosine dimer. , 1971, Journal of molecular biology.

[13]  M. Yaniv,et al.  Photochemistry of 4-thiouridine in Escherichia coli transfer RNA1Val. , 1971, Journal of molecular biology.

[14]  D. Omilianowski,et al.  Cross-Linked Transfer RNA Functions in All Steps of the Translation Process , 1971, Science.

[15]  H. Bremer,et al.  Uridine transport and incorporation into nucleic acids in escherichia coli. , 1968, Biochimica et biophysica acta.

[16]  M. Lipsett.  The isolation of 4-thiouridylic acid from the soluble ribonucleic acid of Escherichia coli. , 1965, The Journal of biological chemistry.

[17]  R. Stafford,et al.  DELAY IN GROWTH AND DIVISION INDUCED BY NEAR ULTRAVIOLET RADIATION IN ESCHERICHIA COLI B AND ITS ROLE IN PHOTOPROTECTION AND LIQUID HOLDING RECOVERY , 1964 .

[18]  G. Stent,et al.  A genetic locus for the regulation of ribonucleic acid synthesis. , 1961, Proceedings of the National Academy of Sciences of the United States of America.

[19]  C. A. Parker,et al.  A new sensitive chemical actinometer - II. Potassium ferrioxalate as a standard chemical actinometer , 1956, Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences.

[20]  M. Stacey,et al.  The nucleic acids of Sarcina lutea. , 1956, Journal of general microbiology.

[21]  J. Jagger,et al.  Mechanism of growth delay induced in Escherichia coli by near ultraviolet radiation. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[22]  D. McCorquodale,et al.  THE PREPARATION OF AMINO ACID-TRANSFER RIBONUCLEIC ACID FROM ESCHERICHIA COLI BY DIRECT PHENOL EXTRACTION OF INTACT CELLS. , 1963, Biochimica et biophysica acta.