The human myocardial two‐dimensional gel protein database: Update 1994

An updated human heart protein two‐dimensional electrophoresis (2‐DE) database is presented. The database, which contains some 1388 protein spots characterised in terms of Mr and pI, has been analysed further by Western immunoblotting and protein sequencing. From a total of 103 protein spots analysed, 49 have been identified by immunoblotting and 32 have been identified by protein sequencing. A further six proteins have tentatively been assigned by comparison with the human heart 2‐DE protein database of Jungblut et al. (Electrophoresis) 1994, 15, 685–607). This database is being used in studies of alterations in protein expression in the diseased and transplanted human heart.

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