The purpose of this study is to examine the effect of heat loss through walls upon the gradients of temperature and contaminant concentration in room with displacement ventilation. It is known that conduction heat loss is governed by outside temperature, heat load inside the room, supply air temperature and overall heat transfer coefficient of walls. Experiments were conducted to measure the temperature gradient and the ventilation efficiency in the room ventilated by displacement ventilation with various combinations of heat load and temperature difference between supply air and outside air. In order to simulate the change of seasons, the supply air temperature was changed instead of the outside air temperature. The effect of supply air temperature and heat generation inside the room on the temperature gradient and the concentration of tracer gas were investigated through the experiments. As a result, it turned out that the higher the heat generation rate and the lower the supply temperature, the stronger the temperature stratification and the lower the concentration in the lower zone. Additionally, ventilation heat loss turned out to be a good index for assessing the concentration in the lower zone. Temperature differences of around 3 degrees C between supply air temperature and exhaust temperature are at least needed for displacement ventilation under the conditions of the experiment presented in this paper.