Ice Nucleation Activity in the Freezing-Tolerant Antarctic NematodePanagrolaimus davidi

Abstract Ice nucleation spectrometry was used to look for the presence of ice nucleating agents (INAs), and their inhibitors, in cultures of Panagrolaimus davidi , an Antarctic nematode which survives intracellular freezing. INA activity was absent in both nematode suspensions and homogenates. The nematodes produce a substance which inhibits the nucleation activity of organic INAs but not of an inorganic INA (AgI). The nucleation inhibitor is both released from the nematode by homogenization and excreted by them into the medium, but the former was more effective at inhibiting nucleation. The inhibitory activity was destroyed by heating. A thermal hysteresis protein, or a similar ice-active substance, may be responsible for the nucleation inhibition.

[1]  W. Block,et al.  Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing. , 1997, Cryobiology.

[2]  P. Wilson,et al.  Recrystallization in a freezing tolerant Antarctic nematode, Panagrolaimus davidi, and an alpine weta, Hemideina maori (Orthoptera; Stenopelmatidae). , 1996, Cryobiology.

[3]  Wharton,et al.  Survival of intracellular freezing by the Antarctic nematode Panagrolaimus davidi , 1995, The Journal of experimental biology.

[4]  D. Wharton.  COLD TOLERANCE STRATEGIES IN NEMATODES , 1995, Biological reviews of the Cambridge Philosophical Society.

[5]  C. Knight,et al.  Nonequilibrium antifreeze peptides and the recrystallization of ice. , 1995, Cryobiology.

[6]  R. Lee,et al.  Survival of intracellular freezing, lipid coalescence and osmotic fragility in fat body cells of the freeze-tolerant gall fly Eurosta solidaginis , 1993 .

[7]  R. Lee,et al.  Insect Cold-hardiness and Ice Nucleating Active Microorganisms Including Their Potential Use for Biological Control , 1993 .

[8]  R. Lee.  Principles of Insect Low Temperature Tolerance , 1991 .

[9]  L. Neven,et al.  Hemolymph Proteins Involved in Insect Subzero-Temperature Tolerance: Ice Nucleators and Antifreeze Proteins , 1991 .

[10]  Richard E. Lee,et al.  Insects at low temperature , 1991 .

[11]  D. Wharton,et al.  Cold-Tolerance Mechanisms of the Antarctic Nematode Panagrolaimus Davidi , 1991 .

[12]  D. Wharton,et al.  A survey of terrestrial nematodes from the McMurdo Sound region, Antarctica , 1989 .

[13]  K. P. Murphy,et al.  Inhibition of bacterial ice nucleators by fish antifreeze glycoproteins , 1988, Nature.

[14]  C. Knight,et al.  Inhibition of recrystallization of ice by insect thermal hysteresis proteins: A possible cryoprotective role , 1986 .

[15]  J. Duman.  Thermal hysteresis antifreeze proteins in the midgut fluid of overwintering larvae of the beetle Dendroides canadensis , 1984 .

[16]  G. Vali.  Quantitative Evaluation of Experimental Results an the Heterogeneous Freezing Nucleation of Supercooled Liquids , 1971 .