Temperature-dependency of electron-transport activity in mitochondria with exogenous mitochondrial DNA in Drosophila.

In mitochondrial DNA (mtDNA) heteroplasmy induced artificially in Drosophila melanogaster (Matsuura et al., 1989), foreign mtDNA derived from D. mauritiana was selectively transmitted at 25 degrees C but was lost at 19 degrees C (Niki et al., 1989; Matsuura et al., 1990, 1991). To investigate temperature-dependent factors in the selective transmission of mtDNA, the temperature-dependency of electron-transport activity of mitochondria from D. melanogaster in which endogenous mtDNA was completely replaced by the foreign mtDNA was compared with that of D. melanogaster and D. mauritiana. For NADH-oxidase activity, the optimum temperature of D. mauritiana mitochondria was 35 degrees C while for two types of mitochondria from D. melanogaster each possessing either endogenous or exogenous mtDNA, maximum activity was noted at 32 degrees C. This observation suggests that the temperature-dependency of mitochondrial electron-transport activity is mainly determined by a nuclear genome. NADH-cytochrome c reductase and cytochrome c oxidase activities were not significantly different among the three types of mitochondria. The temperature-dependency of mitochondrial function apparently is not involved in the temperature-dependent selective transmission of mtDNA in the heteroplasmic state.

[1]  Y. Niki,et al.  Selective transmission of mitochondrial DNA in heteroplasmic lines for intra- and interspecific combinations in Drosophila melanogaster. , 1991, Idengaku zasshi.

[2]  N. Takahata,et al.  Evolution of Drosophila mitochondrial DNA and the history of the melanogaster subgroup. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[3]  Y. Niki,et al.  Differences in the modes of transmission of foreign mitochondrial DNA in heteroplasmic lines for intra- and interspecific combinations in Drosophila melanogaster. , 1990, Idengaku zasshi.

[4]  W. L. Fangman,et al.  RPO41-independent maintenance of [rho-] mitochondrial DNA in Saccharomyces cerevisiae , 1990, Molecular and cellular biology.

[5]  M. King,et al.  Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. , 1989, Science.

[6]  Y. Niki,et al.  Complete replacement of mitochondrial DNA in Drosophila , 1989, Nature.

[7]  Y. Niki,et al.  Induction of mitochondrial DNA heteroplasmy by intra- and interspecific transplantation of germ plasm in Drosophila. , 1989, Genetics.

[8]  P. Slonimski,et al.  An essential yeast protein, encoded by duplicated genes TIF1 and TIF2 and homologous to the mammalian translation initiation factor eIF-4A, can suppress a mitochondrial missense mutation. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[9]  M. Wésolowski-Louvel,et al.  RAG1 and RAG2: Nuclear genes involved in the dependence/independence on mitochondrial respiratory function for growth on sugars , 1989, Yeast.

[10]  T. Fox,et al.  Plasmids can stably transform yeast mitochondria lacking endogenous mtDNA. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[11]  S. Johnston,et al.  Mitochondrial transformation in yeast by bombardment with microprojectiles. , 1988, Science.

[12]  Michael P. King,et al.  Injection of mitochondria into human cells leads to a rapid replacement of the endogenous mitochondrial DNA , 1988, Cell.

[13]  R. O. Poyton,et al.  Identification of a third nuclear protein-coding gene required specifically for posttranscriptional expression of the mitochondrial COX3 gene is Saccharomyces cerevisiae , 1988, Journal of bacteriology.

[14]  Y. Satta,et al.  Analysis of nucleotide substitutions of mitochondrial DNAs in Drosophila melanogaster and its sibling species. , 1987, Molecular biology and evolution.

[15]  D. Mh.  Drosophila melanogaster mitochondrial DNA, a novel organization and genetic code , 1983 .

[16]  H. Gest,et al.  Genetic Mutations Affecting the Respiratory Electron-Transport System of the Photosynthetic Bacterium Rhodopseudomonas capsulata , 1973, Journal of bacteriology.

[17]  J. David,et al.  Drosophila mauritiana n. sp. du groupe melanogaster de l'Ile Maurice [Dipt. Drosophilidae] , 1974, Bulletin de la Société entomologique de France.

[18]  G. Schatz,et al.  The biogenesis of mitochondria. , 1970, The Biochemical journal.