Efficacy of Polymerase Chain Reaction in Diagnosis of Saproleniosis in Oreochromis Niloticus

The present study was carried out on 100 cultured Nile tilapia (O. niloticus) of different ages were collected from farms of Central Laboratory for Aquaculture Research (Abbassa, Abou Hammad, Sharkia, Egypt) during period from late November, 2014 to march, 2015. The collected fish samples were studies for presence of fungal infection. Fish were clinically showed respiratory manifestation, erratic movement, loss of equilibrium, off food and also showed whit to gray patches of filamentous mycelium “cotton like” on fish skin, around head, dorsal and caudal fins, gills, on the muscular layer and eye causing unilateral or bilateral eye opacity. Internally, the diseased fish showed, pale gills, dark and enlarged liver, kidney and intestine free from any food. The collected samples were suspected to mycological and biochemical identification which initially suggestive of Saprolegnia spp. The tradionally identification was supported by using Polymerase Chain Reaction (PCR) which very important rapid and accurate method to identify Saprolegnia from other water mold. Also, in this study, the antifungal effect of ethanolic extract of Ruta graveolens( natural herbal plant) on Saprolegnia was studied in vitro by disk and well diffusion methods. Further by trials, iodine was an effective antifungal disinfectant for infected fish.

[1]  J. Diéguez-Uribeondo,et al.  Molecular identification of a bronopol tolerant strain of Saprolegnia australis causing egg and fry mortality in farmed brown trout, Salmo trutta. , 2014, Fungal biology.

[2]  P. West,et al.  The impact of the water moulds Saprolegnia diclina and Saprolegnia parasitica on natural ecosystems and the aquaculture industry , 2013 .

[3]  M. Abdelsalam,et al.  Detection of Saprolegnia parasitica in eggs of angelfish Pterophyllum scalare (Cuvier–Valenciennes) with a history of decreased hatchability , 2013 .

[4]  K. Revathi,et al.  A simple method for total genomic DNA extraction from water moulds , 2013 .

[5]  A. Shinn,et al.  The efficacy of selected plant extracts and bioflavonoids in controlling infections of Saprolegnia australis (Saprolegniales; Oomycetes) , 2012 .

[6]  P. West,et al.  1 18 Saprolegnia and other Oomycetes , 2012 .

[7]  Karouei S. M. Hashemi,et al.  Isolation of Saprolegnia and the Influence of RootEthanolic Extract of Ruta graveolens on Saprolegnia. SppGrowth , 2012 .

[8]  N. Taha,et al.  Pathological and Biochemical Studies in Tilapia Zilli Infected with Saprolegnia parasitica and Treated with Potassium Permanganate , 2010 .

[9]  K. Peltonen,et al.  Disappearance of malachite green residues in fry of rainbow trout (Oncorhynchus mykiss) after treatment of eggs at the hatching stage , 2009 .

[10]  F. Govers,et al.  Internuclear gene silencing in Phytophthora infestans is established through chromatin remodelling. , 2008, Microbiology.

[11]  D. Pilliod,et al.  Saprolegniaceae identified on amphibian eggs throughout the Pacific Northwest, USA, by internal transcribed spacer sequences and phylogenetic analysis , 2008, Mycologia.

[12]  M. Ortiz-Santaliestra,et al.  Saprolegnia diclina: another species responsible for the emergent disease 'Saprolegnia infections' in amphibians. , 2008, FEMS microbiology letters.

[13]  A. S. Diab,et al.  Saprolegniosis in freshwater cultured tilapia nilotica (Orechromis niloticus) and trial for control by using Bafry D50/500. , 2008 .

[14]  J. Fregeneda-Grandes,et al.  Fungi isolated from cultured eggs, alevins and broodfish of brown trout in a hatchery affected by saprolegniosis , 2007 .

[15]  D. Moran,et al.  An economic evaluation of the control of three notifiable fish diseases in the United Kingdom. , 2007, Preventive veterinary medicine.

[16]  P. West.  Saprolegnia parasitica, an oomycete pathogen with a fishy appetite: new challenges for an old problem , 2006 .

[17]  R. Reimschuessel,et al.  Formalin treatment to reduce mortality associated with Saprolegnia parasitica in rainbow trout, Oncorhynchus mykiss , 2006 .

[18]  Kumudini M. Meepagala,et al.  Algicidal and antifungal compounds from the roots of Ruta graveolens and synthesis of their analogs. , 2005, Phytochemistry.

[19]  K. Hatai,et al.  Morphological and physiological characteristics of Saprolegnia spp. strains pathogenic to Atlantic salmon, Salmo salar L. , 2005, Journal of fish diseases.

[20]  E. Peeler,et al.  A framework for understanding the potential for emerging diseases in aquaculture. , 2005, Preventive veterinary medicine.

[21]  M. Yoshioka,et al.  Fungicidal efficacy of sodium hypochlorite on a fish-pathogen oomycetes, Saprolegnia diclina from Thailand , 2005 .

[22]  L. Gasco,et al.  The use of ozone in trout hatchery to reduce saprolegniasis incidence , 2003 .

[23]  Stephen O Duke,et al.  Natural fungicides from Ruta graveolens L. leaves, including a new quinolone alkaloid. , 2003, Journal of agricultural and food chemistry.

[24]  K. Hatai,et al.  Pathogenicity of Saprolegnia species associated with outbreaks of salmonid saprolegniosis in Japan. , 2002 .

[25]  S. J. Culp,et al.  Mutagenicity and carcinogenicity in relation to DNA adduct formation in rats fed leucomalachite green. , 2002, Mutation research.

[26]  L. Khoo.  Fungal diseases in fish , 2000 .

[27]  E. Noga.  Water mold infections of freshwater fish: Recent advances , 1993 .

[28]  F P Meyer,et al.  Aquaculture disease and health management. , 1991, Journal of animal science.

[29]  T. Kuriakose,et al.  Use of lactophenol cotton blue mounts of corneal scrapings as an aid to the diagnosis of mycotic keratitis. , 1991, Diagnostic microbiology and infectious disease.

[30]  A. D. Pickering,et al.  Viable saprolegniaceae spores on the epidermis of the Salmonid fish Salmo trutta and Salvelinus alpinus , 1977 .