Optoelectrofluidic behavior of metal–polymer hybrid colloidal particles

Behavior of metal–polymer hybrid colloidal particles in an optoelectrofluidic device has been investigated theoretically and experimentally. In the application of hundreds of kHz ac voltage, a variety of optically induced electrokinetic and electrostatic mechanisms affect the movement of gold-coated polystyrene microspheres. The particles repel from the light pattern, and their mobility increases as the amount of gold increases. We apply this model to develop an optoelectrofluidic immunoassay, in which the corresponding metal–polymer hybrid particles are formed by a reaction of antibody-coated gold nanoparticles, antigens, and antibody-coated polystyrene microspheres.

[1]  G. Hermanson.  Immunotoxin Conjugation Techniques , 1996 .

[2]  S. Nie,et al.  Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules , 2001, Nature Biotechnology.

[3]  Zbigniew Adamczyk,et al.  Particle adsorption and deposition: role of electrostatic interactions , 2003 .

[4]  J L West,et al.  A whole blood immunoassay using gold nanoshells. , 2003, Analytical chemistry.

[5]  Ming C. Wu,et al.  Massively parallel manipulation of single cells and microparticles using optical images , 2005, Nature.

[6]  G. Stucky,et al.  Metal–Silica Hybrid Nanostructures for Surface‐Enhanced Raman Spectroscopy , 2006 .

[7]  Zhi-Min Dang,et al.  Dielectric behavior of a metal-polymer composite with low percolation threshold , 2006 .

[8]  M.C. Wu,et al.  Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers , 2007, IEEE Journal of Selected Topics in Quantum Electronics.

[9]  Hyundoo Hwang,et al.  Experimental investigation of electrostatic particle-particle interactions in optoelectronic tweezers. , 2008, The journal of physical chemistry. B.

[10]  Jin Jang,et al.  Reduction of nonspecific surface-particle interactions in optoelectronic tweezers , 2008 .

[11]  Jin Jang,et al.  Interactive manipulation of blood cells using a lens‐integrated liquid crystal display based optoelectronic tweezers system , 2008, Electrophoresis.

[12]  Seong-Won Nam,et al.  Programmable manipulation of motile cells in optoelectronic tweezers using a grayscale image , 2008 .

[13]  M.C. Wu,et al.  Operational Regimes and Physics Present in Optoelectronic Tweezers , 2008, Journal of Microelectromechanical Systems.

[14]  Hyundoo Hwang,et al.  Dynamic light-activated control of local chemical concentration in a fluid. , 2009, Analytical chemistry.

[15]  Do-Hyun Lee,et al.  Enhanced discrimination of normal oocytes using optically induced pulling-up dielectrophoretic force. , 2009, Biomicrofluidics.

[16]  Hyundoo Hwang,et al.  Rapid and selective concentration of microparticles in an optoelectrofluidic platform. , 2009, Lab on a chip.

[17]  Kyoung-Shin Choi,et al.  Cathodic deposition of polypyrrole enabling the one-step assembly of metal-polymer hybrid electrodes. , 2009, Angewandte Chemie.

[18]  Hyundoo Hwang,et al.  Measurement of molecular diffusion based on optoelectrofluidic fluorescence microscopy. , 2009, Analytical chemistry.

[19]  Jung-Hyun Lee,et al.  Facile preparation of highly-scattering metal nanoparticle-coated polymer microbeads and their surface plasmon resonance. , 2009, Journal of the American Chemical Society.

[20]  Hyundoo Hwang,et al.  Optoelectrofluidic control of colloidal assembly in an optically induced electric field. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[21]  Jaebum Choo,et al.  Optoelectrofluidic sandwich immunoassays for detection of human tumor marker using surface-enhanced Raman scattering. , 2010, Analytical chemistry.

[22]  Steven L Neale,et al.  Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers. , 2010, Lab on a chip.

[23]  Yingxi Zhu,et al.  Dielectrophoresis of Janus particles under high frequency ac-electric fields , 2010 .

[24]  Yoon-Kyoung Cho,et al.  In situ dynamic measurements of the enhanced SERS signal using an optoelectrofluidic SERS platform. , 2011, Lab on a chip.

[25]  Je-Kyun Park,et al.  Optoelectrofluidic platforms for chemistry and biology. , 2011, Lab on a chip.

[26]  Shih-Siou Wang,et al.  The application of an optically switched dielectrophoretic (ODEP) force for the manipulation and assembly of cell-encapsulating alginate microbeads in a microfluidic perfusion cell culture system for bottom-up tissue engineering. , 2012, Lab on a chip.