A low cost, easy to build, portable, and universal autosampler for liquids

Abstract Autosamplers are ubiquitous tools in laboratories, and an integral part of many analytical instruments. However, most autosamplers are expensive, and as such they are not used in all laboratories. One option is to purchase an analytical instrument without its autosampler, and integrate an autosampler from another supplier. Using scripting, it is possible to couple any autosampler with any analytical instrument, as long as both have a graphical user interface (GUI). Here we show that it is possible to integrate an inexpensive robotic arm kit, which has a GUI, to any analytical device that also has a GUI. The coupling is simple and does not require any electronic knowledge. We demonstrated that the robotic arm worked as an autosampler with 3 different analytical instruments for 8 different chemical measurements: total alkalinity, pH, total carbon, total organic carbon (including isotopic composition), total inorganic carbon (including isotopic composition) and total nitrogen in water samples. The setup is an economical alternative to the common liquid autosamplers.

[1]  Gilles St-Jean,et al.  Automated quantitative and isotopic (13C) analysis of dissolved inorganic carbon and dissolved organic carbon in continuous-flow using a total organic carbon analyser. , 2003, Rapid communications in mass spectrometry : RCM.

[2]  Joshua M. Pearce.  Open-Source Lab: How to Build Your Own Hardware and Reduce Research Costs , 2013 .

[3]  Joshua M. Pearce.  Laboratory equipment: Cut costs with open-source hardware , 2014, Nature.

[4]  Philip J. Kitson,et al.  Integrated 3D-printed reactionware for chemical synthesis and analysis. , 2012, Nature chemistry.

[5]  F. Grassa,et al.  Stable carbon isotope analysis of dissolved inorganic carbon (DIC) and dissolved organic carbon (DOC) in natural waters--results from a worldwide proficiency test. , 2013, Rapid communications in mass spectrometry : RCM.

[6]  Joshua M. Pearce,et al.  Open-Source 3D-Printable Optics Equipment , 2013, PloS one.

[7]  P. Williams.  Measurement of Dissolved Organic Carbon and Nitrogen in Natural Waters , 1992 .

[8]  Joshua M. Pearce,et al.  Building Research Equipment with Free, Open-Source Hardware , 2012, Science.

[9]  Werner Stumm,et al.  Fresh Water and Ocean. (Book Reviews: Aquatic Chemistry. An Introduction Emphasizing Chemical Equilibria in Natural Waters) , 1982 .

[10]  Matheus C Carvalho,et al.  Integration of Analytical Instruments with Computer Scripting , 2013, Journal of laboratory automation.

[11]  Joshua M. Pearce,et al.  Open-Source Colorimeter , 2013, Sensors.

[12]  Bethany C Gross,et al.  Evaluation of 3D printing and its potential impact on biotechnology and the chemical sciences. , 2014, Analytical chemistry.

[13]  P. Brewer,et al.  High precision measurements of alkalinity and total carbon dioxide in seawater by potentiometric titration — 1. Presence of unknown protolyte(s)? , 1988 .