In Vitro/In Vivo Electrochemical Detection of Pt(II) Species.

The biodistribution of chemotherapy compounds within tumor tissue is one of the main challenges in the development of antineoplastic drugs, and novel techniques for simple, non-expensive, sensitive, and selective detection of various analytes in tumors are of great importance. In this paper we propose the use of platinized carbon nanoelectrodes (PtNE) for electrochemical detection of platinum-based drugs in various biological models, including single cells and tumor spheroids in vitro, and inside solid tumors in vivo. We have demonstrated quantitative direct detection of Pt(II) in breast adenocarcinoma MCF-7 cells treated with cisplatin and cisplatin-based DNP prodrug. To realize the potential of this technique in advanced tumor models, we measured Pt(II) in 3D tumor spheroids in vitro and tumor-bearing mice in vivo. The concentration gradient of Pt (II) species correlated with the distance from the sample surface in MCF-7 tumor spheroids. We then performed detection of Pt(II) species in tumor-bearing mice treated intravenously with cisplatin and DNP. We found that there was deeper penetration of DNP in comparison to cisplatin. This research demonstrates a novel minimally invasive, real-time electrochemical technique for the study of platinum-based drugs.

[1]  M. Čemažar,et al.  High spatial resolution imaging of cisplatin and Texas Red cisplatin in tumour spheroids using laser ablation isotope dilution inductively coupled plasma mass spectrometry and confocal fluorescence microscopy. , 2021, Analytica chimica acta.

[2]  A. Majouga,et al.  Alternative mechanism of action of the DNP PtIV prodrug: intracellular cisplatin release and the mitochondria-mediated apoptotic pathway. , 2021, Dalton transactions.

[3]  Y. Korchev,et al.  Mapping mechanical properties of living cells at nanoscale using intrinsic nanopipette-sample force interactions. , 2021, Nanoscale.

[4]  Y. Ivanenkov,et al.  Discovery of Bivalent GalNAc-Conjugated Betulin as a Potent ASGPR-Directed Agent against Hepatocellular Carcinoma. , 2021, Bioconjugate chemistry.

[5]  A. Ewing,et al.  Chemical Analysis of Single Cells and Organelles , 2020, Analytical chemistry.

[6]  D. A. Grishin,et al.  New Small-Molecule Glycoconjugates of Docetaxel and GalNAc for Targeted Delivery to Hepatocellular Carcinoma. , 2020, Molecular pharmaceutics.

[7]  P. Perego,et al.  The rediscovery of platinum-based cancer therapy , 2020, Nature reviews. Cancer.

[8]  V. N. Nikitina,et al.  NOVEL COPPER-CONTAINING CYTOTOXIC AGENTS BASED ON 2-THIOXOIMIDAZOLONES. , 2020, Journal of medicinal chemistry.

[9]  Zijian Guo,et al.  Multispecific Platinum(IV) Complex Deters Breast Cancer via Interposing Inflammation and Immunosuppression as an Inhibitor of COX-2 and PD-L1. , 2020, Angewandte Chemie.

[10]  M. Nachtigal,et al.  Electrochemical Characterization of Carboplatin at Unmodified Platinum Electrodes and Its Application to Drug Uptake Studies in Ovarian Cancer Cells , 2020, ECS Meeting Abstracts.

[11]  A. Ewing,et al.  Nanoelectrochemical analysis inside a single living cell , 2020 .

[12]  Y. Korchev,et al.  Selective Sensing of Proteins Using Aptamer Functionalized Nanopore Extended Field‐Effect Transistors , 2020 .

[13]  G. Koellensperger,et al.  Laser ablation-ICP-TOFMS imaging of germ cell tumors of patients undergoing platinum-based chemotherapy. , 2020, Metallomics : integrated biometal science.

[14]  B. Jackson,et al.  Effects of Formalin Fixation on Trace Element Concentrations in Bottlenose Dolphin (Tursiops truncatus) Tissues , 2020, Environmental toxicology and chemistry.

[15]  D. Klenerman,et al.  High-resolution label-free 3D mapping of extracellular pH of single living cells , 2019, Nature Communications.

[16]  M. Gholivand,et al.  A novel voltammetric sensor based on graphene quantum dots-thionine/nano-porous glassy carbon electrode for detection of cisplatin as an anti-cancer drug , 2019, Sensors and Actuators B: Chemical.

[17]  E. Khaydukov,et al.  Photodynamic therapy of melanoma by blue-light photoactivation of flavin mononucleotide , 2019, Scientific Reports.

[18]  Weihua Huang,et al.  Electrochemical Monitoring of ROS/RNS Homeostasis Within Individual Phagolysosomes Inside Single Macrophages. , 2019, Angewandte Chemie.

[19]  Y. Long,et al.  Confined Nanopipette Sensing: From Single Molecules, Single Nanoparticles, to Single Cells. , 2019, Angewandte Chemie.

[20]  Keke Hu,et al.  Electrochemical Measurements of Reactive Oxygen and Nitrogen Species inside Single Phagolysosomes of Living Macrophages. , 2019, Journal of the American Chemical Society.

[21]  Keke Hu,et al.  Cavity Carbon-Nanopipette Electrodes for Dopamine Detection. , 2019, Analytical chemistry.

[22]  Y. Korchev,et al.  Novel method for rapid toxicity screening of magnetic nanoparticles , 2018, Scientific Reports.

[23]  G. Zachariadis,et al.  Determination of Cisplatin and Carboplatin Anticancer Drugs by Non-suppressed Ion Chromatography with an Inductively Coupled Plasma Atomic Emission Detector , 2018 .

[24]  Keke Hu,et al.  Direct Electrochemical Measurements of Reactive Oxygen and Nitrogen Species in Nontransformed and Metastatic Human Breast Cells. , 2017, Journal of the American Chemical Society.

[25]  K. Maeda,et al.  A microsensing system for the in vivo real-time detection of local drug kinetics , 2017, Nature Biomedical Engineering.

[26]  Bart Vekemans,et al.  Assessment of Ovarian Cancer Tumors Treated with Intraperitoneal Cisplatin Therapy by Nanoscopic X-ray Fluorescence Imaging , 2016, Scientific Reports.

[27]  T. Matsue,et al.  Spearhead Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis. , 2016, ACS nano.

[28]  S. Lippard,et al.  The Next Generation of Platinum Drugs: Targeted Pt(II) Agents, Nanoparticle Delivery, and Pt(IV) Prodrugs. , 2016, Chemical reviews.

[29]  J. Ščančar,et al.  Speciation of cisplatin in environmental water samples by hydrophilic interaction liquid chromatography coupled to inductively coupled plasma mass spectrometry. , 2015, Talanta.

[30]  Juewen Liu,et al.  Multi-walled carbon nanotubes modified screen-printed electrodes for cisplatin detection , 2015 .

[31]  O. Ornatsky,et al.  Single‐cell measurement of the uptake, intratumoral distribution and cell cycle effects of cisplatin using mass cytometry , 2015, International journal of cancer.

[32]  Juewen Liu,et al.  Glutathione-s-transferase modified electrodes for detecting anticancer drugs. , 2014, Biosensors & bioelectronics.

[33]  David Klenerman,et al.  Electrochemical nanoprobes for single-cell analysis. , 2014, ACS nano.

[34]  Monica Gulati,et al.  Development and Validation of a Simple and Sensitive Spectrometric Method for Estimation of Cisplatin Hydrochloride in Tablet Dosage Forms: Application to Dissolution Studies , 2013 .

[35]  Nicole S. Bryce,et al.  Getting to the core of platinum drug bio-distributions: the penetration of anti-cancer platinum complexes into spheroid tumour models. , 2012, Metallomics : integrated biometal science.

[36]  R. Weigert,et al.  Intravital microscopy , 2012, Bioarchitecture.

[37]  Jean-Marc Noël,et al.  Nanoelectrodes for determination of reactive oxygen and nitrogen species inside murine macrophages , 2012, Proceedings of the National Academy of Sciences.

[38]  Shilpi Agarwal,et al.  Voltammetric techniques for the assay of pharmaceuticals--a review. , 2011, Analytical biochemistry.

[39]  D. Klenerman,et al.  Nanoscale live-cell imaging using hopping probe ion conductance microscopy , 2009, Nature Methods.

[40]  F. S. Rojas,et al.  Analytical methodologies for the determination of cisplatin. , 2008, Journal of pharmaceutical and biomedical analysis.

[41]  H. Erb,et al.  The effects of formalin fixation and tissue embedding of bovine liver on copper, iron, and zinc analysis , 2008, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[42]  François O. Laforge,et al.  Nanoelectrochemistry of mammalian cells , 2008, Proceedings of the National Academy of Sciences.

[43]  René Kizek,et al.  Cisplatin electrochemical biosensor , 2006 .

[44]  F. Zunino,et al.  Electron Microscopy Analysis of Early Localization of Cisplatin in Ovarian Carcinoma Cells , 2002, Ultrastructural pathology.

[45]  Rakesh K. Jain,et al.  Interstitial pH and pO2 gradients in solid tumors in vivo: High-resolution measurements reveal a lack of correlation , 1997, Nature Medicine.

[46]  A. Majouga,et al.  New Fe-Cu bimetallic coordination compounds based on ω-ferrocene carboxylic acids and 2-thioimidazol-4-ones: structural, mechanistic and biological studies , 2021, Inorganic Chemistry Frontiers.

[47]  V. Naumenko,et al.  Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice. , 2016, Methods in molecular biology.

[48]  S. Berners‐Price,et al.  The Chemistry of Cisplatin in Aqueous Solution , 2000 .