Flow Cytometric Analysis of DNA Ploidy in Canine Mammary Tumors1

DNA ploidy has been determined using flow cytometry in 23 nonmalignant and 34 malignant (primary and metastatic) mammary tumors from 46 dogs. This parameter was compared with clinical stage, histology, and estrogen and progesterone receptor analysis. Twenty-one of 34 cancers (61.8%) from 32 dogs were DNA aneuploid. Aneuploidy was also found in 4 of 23 nonmalignant tumors (17.4%) from 20 dogs. Regional lymph nodes were involved in 6 of 10 diploid and 3 of 9 aneuploid cancers of dogs with operable disease. The aneuploidy incidence was higher in dogs that had distant metastasis at initial diagnosis (8 of 11) than in those presented with local or locoregional disease (9 of 19), although this difference was not statistically significant. DNA aneuploidy incidence was not found to be related to histological tumor type, histológica! malignancy grade, nuclear grade, or steroid receptor presence. Hetero geneity in DNA content was found in 4 of 32 cancers (30 dogs) in samples from primary or locally recurrent lesions. In 3 of 16 cancers that were analyzed both at the primary and at secondary sites of growth, a signifi cant variation in DNA content was observed. The degree of aneuploidy in the dog cancers was much lower than seen for human breast carcinomas with a relatively high frequency of hypoploid stemlines (7 of 34 cancers, 20.6%). The frequency distribution of DNA indices in dog mammary cancers indicates that aneuploidy evolution probably differs from that of human breast cancer. tetraploid a 1.90 2.10 G2-M cells of tetraploid cell population more than one aneuploid G0-Gi population was present the classified as multiploid.Statistics. Differences in frequency distribution in data groups assessed with the x2 or Fisher's Exact Test. level of significance set at a P of 0.05.

[1]  H. Muss,et al.  Relationship of flow cytometry results to clinical and steroid receptor status in human breast cancer , 1985, Breast Cancer Research and Treatment.

[2]  J. Raynaud,et al.  Animal models for hormone-dependent human breast cancer , 2004, Cancer Chemotherapy and Pharmacology.

[3]  G. Auer,et al.  Mammary carcinoma: DNA analysis in areas showing different histological features in the same tumor. , 2009, Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, Pathology.

[4]  G. Rutteman,et al.  Prolactin binding in benign and malignant mammary tissue of female dogs. , 1986, Anticancer research.

[5]  W. Misdorp,et al.  Steroid Receptor Determinations in Malignant Mammary Tumors and in Nonaffected Mammary Glands in the Dog , 1986 .

[6]  R. Sager Genetic suppression of tumor formation: a new frontier in cancer research. , 1986, Cancer research.

[7]  J. Mark,et al.  Cytogenetical observations in 100 human benign pleomorphic adenomas: specificity of the chromosomal aberrations and their relationship to sites of localized oncogenes. , 1986, Anticancer research.

[8]  Robert W. McDivitt,et al.  A proposed classification of breast cancer based on kinetic information derived from a comparison of risk factors in 168 primary operable breast cancers , 1986, Cancer.

[9]  D. Horsfall,et al.  Relationship between ploidy and steroid hormone receptors in primary invasive breast cancer. , 1986, British Journal of Cancer.

[10]  S. Fosså,et al.  Primary breast cancer. Flow cytometric DNA pattern in relation to clinical and histopathologic characteristics. , 1986, Cancer.

[11]  G. Roos,et al.  DNA content in renal cell carcinoma with reference to tumor heterogeneity , 1985, Cancer.

[12]  A. Knudson Hereditary cancer, oncogenes, and antioncogenes. , 1985, Cancer research.

[13]  I. Taylor,et al.  Influence of cellular DNA content on disease-free survival of Stage II breast cancer patients. , 1984, Cancer research.

[14]  M. Black,et al.  Correlation of cell‐cycle kinetics, hormone receptors, histopathology, and nodal status in human breast cancer , 1984, Cancer.

[15]  D. Seckinger,et al.  Prognostic indicators including DNA histogram type, receptor content, and staging related to human breast cancer patient survival. , 1984, Cancer research.

[16]  B. Sundelin,et al.  Progression of mammary adenocarcinomas as reflected by nuclear DNA content. , 1984, Cytometry.

[17]  H. Poulsen,et al.  Ploidy level of human breast carcinoma. Relation to histopathologic features and hormone receptor content. , 1984, Acta radiologica. Oncology.

[18]  N. Petrelli,et al.  Flow cytometric analysis of DNA aneuploidy in primary and metastatic human solid tumors. , 1984, Cytometry.

[19]  T. Caspersson,et al.  Prognostic significance of nuclear DNA content in mammary adenocarcinomas in humans. , 1984, Cancer research.

[20]  I W Taylor,et al.  Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[21]  Tanke Hj,et al.  DNA ploidy analysis and cytologic examination of sorted cell populations from human breast tumors. , 1983 .

[22]  B. Barlogie,et al.  Flow cytometry in clinical cancer research. , 1983, Cancer research.

[23]  I. Christensen,et al.  A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. , 1983, Cytometry.

[24]  I J Christensen,et al.  Standardization of high-resolution flow cytometric DNA analysis by the simultaneous use of chicken and trout red blood cells as internal reference standards. , 1983, Cytometry.

[25]  M. Melamed,et al.  Flow cytometry of breast carcinoma. III. Possible altered kinetics in axillary lymph node metastases. , 1982, Analytical and quantitative cytology.

[26]  P. Bichel,et al.  Estrogen receptor content and ploidy of human mammary carcinoma , 1982, Cancer.

[27]  B. Barlogie,et al.  Ploidy, proliferative activity and estrogen receptor content in human breast cancer. , 1982, Cytometry.

[28]  M. Melamed,et al.  Flow cytometry of breast carcinoma: I. Relation of DNA ploidy level to histology and estrogen receptor , 1981, Cancer.

[29]  T. S. Johnson,et al.  Ploidy and DNA distribution analysis of spontaneous dog tumors by flow cytometry. , 1981, Cancer research.

[30]  R. Kay,et al.  Prognostic significance of modal DNA value and other factors in malignant tumours, based on 1465 cases. , 1979, British Journal of Cancer.

[31]  L. Owen A comparative study of canine and human breast cancer. , 1979, Investigative & cell pathology.

[32]  E. Fisher,et al.  Karyotypic abnormalities in precursor lesions of human cancer of the breast. , 1978, American journal of clinical pathology.

[33]  A. Hart,et al.  Prognostic factors in canine mammary cancer. , 1976, Journal of the National Cancer Institute.

[34]  P. Moorhead,et al.  The Giemsa banding pattern of the canine karyotype. , 1975, Cytogenetics and cell genetics.

[35]  J. Hampe,et al.  Tumours and dysplasias of the mammary gland. , 1974, Bulletin of the World Health Organization.

[36]  K. Shimoto,et al.  Karyotypic alteration associated with radiation-induced and spontaneously occurring canine osteosarcomas , 1972 .

[37]  G. Rabotti Ploidy of Primary and Metastatic Human Tumours , 1959, Nature.