Anode microbial communities produced by changing from microbial fuel cell to microbial electrolysis cell operation using two different wastewaters.

Conditions in microbial fuel cells (MFCs) differ from those in microbial electrolysis cells (MECs) due to the intrusion of oxygen through the cathode and the release of H(2) gas into solution. Based on 16S rRNA gene clone libraries, anode communities in reactors fed acetic acid decreased in species richness and diversity, and increased in numbers of Geobacter sulfurreducens, when reactors were shifted from MFCs to MECs. With a complex source of organic matter (potato wastewater), the proportion of Geobacteraceae remained constant when MFCs were converted into MECs, but the percentage of clones belonging to G. sulfurreducens decreased and the percentage of G. metallireducens clones increased. A dairy manure wastewater-fed MFC produced little power, and had more diverse microbial communities, but did not generate current in an MEC. These results show changes in Geobacter species in response to the MEC environment and that higher species diversity is not correlated with current.

[1]  D. Lovley,et al.  Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism , 1994, Applied and environmental microbiology.

[2]  R. Oremland,et al.  Electricity generation by anaerobic bacteria and anoxic sediments from hypersaline soda lakes , 2008, Extremophiles.

[3]  Prathap Parameswaran,et al.  Selecting anode-respiring bacteria based on anode potential: phylogenetic, electrochemical, and microscopic characterization. , 2009, Environmental science & technology.

[4]  D. Cao,et al.  Electricity generation and modeling of microbial fuel cell from continuous beer brewery wastewater. , 2009, Bioresource technology.

[5]  B. Logan,et al.  Graphite fiber brush anodes for increased power production in air-cathode microbial fuel cells. , 2007, Environmental science & technology.

[6]  Bruce E Logan,et al.  Effectiveness of domestic wastewater treatment using microbial fuel cells at ambient and mesophilic temperatures. , 2010, Bioresource technology.

[7]  Jurg Keller,et al.  Efficient hydrogen peroxide generation from organic matter in a bioelectrochemical system , 2009 .

[8]  S. Patil,et al.  Electricity generation using chocolate industry wastewater and its treatment in activated sludge based microbial fuel cell and analysis of developed microbial community in the anode chamber. , 2009, Bioresource technology.

[9]  In S. Kim,et al.  Biohydrogen production via biocatalyzed electrolysis in acetate-fed bioelectrochemical cells and microbial community analysis , 2008 .

[10]  Bruce E Logan,et al.  Long-term cathode performance and the microbial communities that develop in microbial fuel cells fed different fermentation endproducts. , 2011, Bioresource technology.

[11]  B. Logan.  Exoelectrogenic bacteria that power microbial fuel cells , 2009, Nature Reviews Microbiology.

[12]  Derek R. Lovley,et al.  Evidence for Involvement of an Electron Shuttle in Electricity Generation by Geothrix fermentans , 2005, Applied and Environmental Microbiology.

[13]  D. R. Bond,et al.  Electricity Production by Geobacter sulfurreducens Attached to Electrodes , 2003, Applied and Environmental Microbiology.

[14]  S H A O A N C H E N G, † H U B E R T U,et al.  Microbial Electrolysis Cells for High Yield Hydrogen Gas Production from Organic Matter , 2008 .

[15]  W. Verstraete,et al.  Microbial fuel cells generating electricity from rhizodeposits of rice plants. , 2008, Environmental science & technology.

[16]  R. Amann.  In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes , 1995 .

[17]  W. Verstraete,et al.  Microbial phenazine production enhances electron transfer in biofuel cells. , 2005, Environmental science & technology.

[18]  J. Hughes,et al.  Counting the Uncountable: Statistical Approaches to Estimating Microbial Diversity , 2001, Applied and Environmental Microbiology.

[19]  Bruce E. Logan,et al.  Hydrogen Production by Geobacter Species and a Mixed Consortium in a Microbial Electrolysis Cell , 2009, Applied and Environmental Microbiology.

[20]  S. Giovannoni,et al.  Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals , 2004, Archives of Microbiology.

[21]  D. Lovley.  The microbe electric: conversion of organic matter to electricity. , 2008, Current opinion in biotechnology.

[22]  Bruce E. Logan,et al.  Hydrogen production with nickel powder cathode catalysts in microbial electrolysis cells , 2010 .

[23]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[24]  I. Good.  THE POPULATION FREQUENCIES OF SPECIES AND THE ESTIMATION OF POPULATION PARAMETERS , 1953 .

[25]  Bruce E. Logan,et al.  Increased performance of single-chamber microbial fuel cells using an improved cathode structure , 2006 .

[26]  Bruce E Logan,et al.  Hydrogen production in a single chamber microbial electrolysis cell lacking a membrane. , 2008, Environmental science & technology.

[27]  C R Woese,et al.  A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. , 1991, Systematic and applied microbiology.

[28]  Hong Liu,et al.  Hydrogen production using single-chamber membrane-free microbial electrolysis cells. , 2008, Water research.

[29]  B. Logan,et al.  Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera , 2010, Applied Microbiology and Biotechnology.

[30]  B. Logan,et al.  Brewery wastewater treatment using air-cathode microbial fuel cells , 2008, Applied Microbiology and Biotechnology.

[31]  Liping Huang,et al.  Electricity generation and treatment of paper recycling wastewater using a microbial fuel cell , 2008, Applied Microbiology and Biotechnology.

[32]  Michael Wagner,et al.  Bacterial community composition and function in sewage treatment systems. , 2002, Current opinion in biotechnology.

[33]  M. Bianchi,et al.  Statistical sampling of bacterial strains and its use in bacterial diversity measurement , 1982, Microbial Ecology.

[34]  Justin C. Biffinger,et al.  Oxygen exposure promotes fuel diversity for Shewanella oneidensis microbial fuel cells. , 2008, Biosensors & bioelectronics.

[35]  K. Xiao,et al.  A new method for water desalination using microbial desalination cells. , 2009, Environmental science & technology.

[36]  Bruce E. Logan,et al.  Hydrogen production from cellulose in a two-stage process combining fermentation and electrohydrogenesis , 2009 .

[37]  Bruce E Logan,et al.  Power production in MFCs inoculated with Shewanella oneidensis MR‐1 or mixed cultures , 2010, Biotechnology and bioengineering.

[38]  Xia Huang,et al.  Separator characteristics for increasing performance of microbial fuel cells. , 2009, Environmental science & technology.

[39]  Bruce E Logan,et al.  Direct biological conversion of electrical current into methane by electromethanogenesis. , 2009, Environmental science & technology.

[40]  Kelly P. Nevin,et al.  Lack of Electricity Production by Pelobacter carbinolicus Indicates that the Capacity for Fe(III) Oxide Reduction Does Not Necessarily Confer Electron Transfer Ability to Fuel Cell Anodes , 2007, Applied and Environmental Microbiology.