Stability Indicating RP-HPLC Method for Estimation of Thiocolchicoside in Capsule Dosage Forms

A rapid, simple and precise stability indicating RP-HPLC method for the determination of Thiocolchicoside on C18 column (250mm × 4mm, 5μm) has been developed. A mobile phase consisting of acetonitrile: water (70:30) was used. The flow rate was 1.0 mL min-1 with UV detection at 286 nm.The specificity of the method was ascertained by forced degradation studies by acid and alkali degradation, oxidation and photolysis. Beer Law is obeyed over a concentration range of 0-10μg/ml and correlation coefficient as 0.9996. This method was also