The essential mosquito-stage P25 and P28 proteins from Plasmodium form tile-like triangular prisms

P25 and P28 proteins are essential for Plasmodium parasites to infect mosquitoes and are leading candidates for a transmission-blocking malaria vaccine. The Plasmodium vivax P25 is a triangular prism that could tile the parasite surface. The residues forming the triangle are conserved in P25 and P28 from all Plasmodium species. A cocrystal structure shows that a transmission-blocking antibody uses only its heavy chain to bind Pvs25 at a vertex of the triangle.

[1]  J. Sattabongkot,et al.  EVALUATION OF PROCEDURES TO DETERMINE ABSOLUTE DENSITY OF PLASMODIUM VIVAX OOKINETES , 2005, The Journal of parasitology.

[2]  D. Kaslow,et al.  Antibodies to Malaria Vaccine Candidates Pvs25 and Pvs28 Completely Block the Ability of Plasmodium vivax To Infect Mosquitoes , 2000, Infection and Immunity.

[3]  D. Kaslow,et al.  Crystallization of an intact monoclonal antibody (4B7) against Plasmodium falciparum malaria with peptides from the Pfs25 protein antigen. , 1994, Acta crystallographica. Section D, Biological crystallography.

[4]  M. Lawrence,et al.  Shape complementarity at protein/protein interfaces. , 1993, Journal of molecular biology.

[5]  J. Sattabongkot,et al.  Phase 1 vaccine trial of Pvs25H: a transmission blocking vaccine for Plasmodium vivax malaria. , 2005, Vaccine.

[6]  J. Meuwissen,et al.  Identification of a peptide sequence of the 25 kD surface protein of Plasmodium falciparum recognized by transmission‐blocking monoclonal antibodies: implications for synthetic vaccine development , 1989, Parasite immunology.

[7]  R. Sinden,et al.  P25 and P28 proteins of the malaria ookinete surface have multiple and partially redundant functions , 2001, The EMBO journal.

[8]  R. Sinden,et al.  Characterization of the modes of action of anti-Pbs21 malaria transmission-blocking immunity: ookinete to oocyst differentiation in vivo , 1994, Parasitology.

[9]  R. Sinden.  A proteomic analysis of malaria biology: integration of old literature and new technologies. , 2004, International journal for parasitology.

[10]  C Sander,et al.  Mapping the Protein Universe , 1996, Science.

[11]  C. Janse,et al.  Complement-Like Protein TEP1 Is a Determinant of Vectorial Capacity in the Malaria Vector Anopheles gambiae , 2004, Cell.