Waveguide interrogated optical immunosensor (WIOS) for detection of sulfonamide antibiotics in milk.

An immunosensor was developed for the detection of sulfonamide antibiotics in milk. Detection relied on a competitive immunoassay format, using immunoreagents previously developed for the generic detection of sulfonamide antibiotics and evaluated by enzyme-linked immunosorbent assay. The immunoassay was implemented onto a microsystem platform, the wavelength interrogated optical sensing device, which uses the evanescent field to probe changes at the interface of a waveguiding layer, and thus allows sensitive detection of biomolecule adsorption. The immunoreagents were immobilized onto the surface of the waveguide chip, and a fluidic cell allowed flowing analyte and detection solutions above the surface. Sulfapyridine was used as reference sulfonamide and detected with the immunosensor in buffer and in milk with a limit of detection (IC(90)) of 0.2+/-0.1 microg L(-1) and 0.5+/-0.1 microg L(-1), respectively. The analysis time was below 30 min, including regeneration of the sensing surface, with minimum sample preparation required. The reproducibility of the detection was better than 10%. A blind assay allowed identifying milk samples that were contaminated with different sulfonamide antibiotics at or above the maximum residue limits established by the European Union for these compounds (100 microg L(-1)). Thus, the developed immunosensor presents great potential as a generic sensing device for the fast and early detection of food contaminants on the field by non-skilled users.

[1]  Antje J Baeumner,et al.  Biosensors for environmental pollutants and food contaminants , 2003, Analytical and bioanalytical chemistry.

[2]  Damià Barceló,et al.  Part per trillion determination of atrazine in natural water samples by a surface plasmon resonance immunosensor , 2007, Analytical and bioanalytical chemistry.

[3]  G. Stoev,et al.  Quantitative determination of sulfonamide residues in foods of animal origin by high-performance liquid chromatography with fluorescence detection. , 2000, Journal of chromatography. A.

[4]  Guenter Gauglitz,et al.  Direct optical sensors: principles and selected applications , 2005, Analytical and bioanalytical chemistry.

[5]  P M Nellen,et al.  Model experiments with integrated optical input grating couplers as direct immunosensors. , 1991, Biosensors & bioelectronics.

[6]  Namsoo Kim,et al.  Salmonella detection with a direct-binding optical grating coupler immunosensor , 2007 .

[7]  W. Lukosz,et al.  Sensitivity of grating couplers as integrated-optical chemical sensors , 1989 .

[8]  R. Kunz,et al.  Label-free highly sensitive detection of (small) molecules by wavelength interrogation of integrated optical chips , 2003 .

[9]  Broad-band wavelength-interrogated waveguide sensor , 2006 .

[10]  J. Ramón‐Azcón,et al.  An impedimetric immunosensor based on interdigitated microelectrodes (IDmicroE) for the determination of atrazine residues in food samples. , 2008, Biosensors & bioelectronics.

[11]  Dingren Bi,et al.  Development of an immunochromatographic lateral-flow test strip for rapid detection of sulfonamides in eggs and chicken muscles. , 2007, Journal of agricultural and food chemistry.

[12]  Craig A. Grimes,et al.  Encyclopedia of Sensors , 2006 .

[13]  Lothar U. Kempen,et al.  Miniature integrated optical sensors , 1994, Other Conferences.

[14]  F. Sánchez-Baeza,et al.  Development of an immunochemical technique for the analysis of trichlorophenols using theoretical models. , 2000, Analytical chemistry.

[15]  G. Wyatt,et al.  Molecular modeling of hapten structure and relevance to broad specificity immunoassay of sulfonamide antibiotics. , 1999, Bioconjugate chemistry.

[16]  Romain Quidant,et al.  Quantitative detection of doping substances by a localised surface plasmon sensor. , 2006, Biosensors & bioelectronics.

[17]  Dong-Kyung Kim,et al.  Sulfamethazine detection with direct-binding optical waveguide lightmode spectroscopy-based immunosensor. , 2008, Food chemistry.

[18]  F. Ricci,et al.  A review on novel developments and applications of immunosensors in food analysis. , 2007, Analytica chimica acta.

[19]  D. Smith,et al.  Animal growth promoters: to ban or not to ban? A risk assessment approach. , 2004, International journal of antimicrobial agents.

[20]  Namsoo Kim,et al.  Development of new immunosensors for determination of contaminants in food , 2006 .

[21]  V. Reeves.  Confirmation of multiple sulfonamide residues in bovine milk by gas chromatography-positive chemical ionization mass spectrometry. , 1999, Journal of chromatography. B, Biomedical sciences and applications.

[22]  E. Schacht,et al.  Extraction procedure for sulfachloropyridazine in porcine tissues and detection in a sulfonamide-specific enzyme-linked immunosorbent assay (ELISA) , 2003 .

[23]  R. Kunz,et al.  Optimizing integrated optical chips for label-free (bio-)chemical sensing , 2006, Analytical and bioanalytical chemistry.

[24]  D Barceló,et al.  Influence of the hapten design on the development of a competitive ELISA for the determination of the antifouling agent Irgarol 1051 at trace levels. , 1998, Analytical chemistry.

[25]  Jean-Marc Diserens,et al.  A multianalyte ELISA for immunochemical screening of sulfonamide, fluoroquinolone and ß-lactam antibiotics in milk samples using class-selective bioreceptors , 2008, Analytical and bioanalytical chemistry.

[26]  R. Compañó,et al.  Analysis of trace levels of sulfonamides in surface water and soil samples by liquid chromatography-fluorescence. , 2007, Journal of chromatography. A.

[27]  H. C. Pedersen,et al.  Monitoring of living cell attachment and spreading using reverse symmetry waveguide sensing , 2005 .

[28]  M. Savoy-Perroud,et al.  Validation and comparison of the Copan Milk Test and Delvotest SP-NT for the detection of antimicrobials in milk. , 2007, Analytica chimica acta.

[29]  N. Furusawa.  Rapid high-performance liquid chromatographic determining technique of sulfamonomethoxine, sulfadimethoxine, and sulfaquinoxaline in eggs without use of organic solvents , 2003 .

[30]  F. Sánchez-Baeza,et al.  Generation of broad specificity antibodies for sulfonamide antibiotics and development of an enzyme-linked immunosorbent assay (ELISA) for the analysis of milk samples. , 2009, Journal of agricultural and food chemistry.

[31]  Daniel G. Pinacho,et al.  Labeless Immunosensor Assay for Fluoroquinolone Antibiotics Based Upon an AC Impedance Protocol , 2007 .

[32]  R E Kunz,et al.  Wavelength-interrogated optical sensor for biochemical applications. , 2000, Optics letters.

[33]  H. Wegener.  Antibiotics in animal feed and their role in resistance development. , 2003, Current opinion in microbiology.

[34]  J. Ridet,et al.  Glycoprofiling with micro-arrays of glycoconjugates and lectins. , 2004, Glycobiology.

[35]  Hans Sigrist,et al.  Protein Density Gradients on Surfaces , 2002 .

[36]  Rino E. Kunz,et al.  Sensing pads for hybrid and monolithic integrated optical immunosensors , 1995, Other Conferences.

[37]  F. Sánchez-Baeza,et al.  Immunochemical assays for direct sulfonamide antibiotic detection in milk and hair samples using antibody derivatized magnetic nanoparticles. , 2008, Journal of agricultural and food chemistry.

[38]  H. Ache,et al.  Covalent photolinker-mediated immobilization of an intermediate dextran layer to polymer-coated surfaces for biosensing applications. , 1998, Biosensors & bioelectronics.