Comparison of the Voltammetric Behavior of Metronidazole at a DNA-Modified Glassy Carbon Electrode, a Mercury Thin Film Electrode and a Glassy Carbon Electrode

The electroanalytical performance at three electrodes: DNA-modified glassy carbon electrode, mercury thin film electrode and glassy carhon electrode, for the study of the electrochemical reduction of metronidazole is compared. All three electrodes showed a similar trend in the reduction mechanism for metronidazole, dependent on pH in the acid and neutral region and independent in alkaline media, although there was a shift in the peak potentials to more negative values when a bare glassy carhon electrode was used compared to the other two. Besides the advantage of using a solid electrode for the reduction of metronidazole, the DNA-modified glassy carbon electrode enables a lower detection limit of l.0pM owing to the preconcentration of the drug on the electrode surface, which is not the case for the mercury thin film or bare glassy carhon electrodes.

[1]  J. Tocher,et al.  Evidence for the direct interaction of reduced metronidazole derivatives with DNA bases. , 1994, Biochemical pharmacology.

[2]  P. Zuman,et al.  Polarographic and electrochemical studies of some aromatic and heterocyclic nitro compounds Part 9. Substitutent effects on protonation of the radical ArNO2H· and its reactions with hydroxylamino and nitroso compounds in buffered mixtures of water and DMF , 1995 .

[3]  J. Monteagudo,et al.  Electrochemistry of Ornidazole , 1993 .

[4]  J. Volke,et al.  Simultaneous polarographic determination of 1‐(2‐hydroxyethyl)‐2‐methyl‐5‐nitroimidazole, 1‐(2‐hydroxyethyl)‐2‐methyl‐4‐nitroimidazole and 2‐methyl‐5‐nitroimidazole , 1966, The Journal of pharmacy and pharmacology.

[5]  E. Paleček,et al.  Biopolymer-modified electrodes in the voltammetric determination of nucleic acids and proteins at the submicrogram level , 1993 .

[6]  A. Z. Zuhri,et al.  Electrochemical Reduction of Metronidazole and Its Determination in Pharmaceutical Dosage Forms by D.C. Polarography , 1986 .

[7]  Z. Wang,et al.  Study on the determination of metronidazole in human serum by adsorptive stripping voltammetry. , 1993, Talanta.

[8]  E. Paleček,et al.  Cyclic voltammetry of submicrogram quantities of supercoiled, linear and denatured DNAs with DNA-modified mercury electrode. , 1993, Journal of biomolecular structure & dynamics.

[9]  S. Leach,et al.  Polarographic analysis of heterocyclic nitrogen compounds , 1981 .

[10]  Y. Chien,et al.  Activity-electroreduction relationship of antimicrobial metronidazole analogues. , 1978, Journal of medicinal chemistry.

[11]  D. Sužnjević,et al.  Polarographic and electrochemical studies of some aromatic and heterocyclic nitro compounds, part III: Electroreduction of mono- and dinitropyrazoles and -imidazoles , 1992 .

[12]  P. Declerck,et al.  Polarographic evidence for the interaction of reduced nitroimidazole derivatives with DNA bases , 1987 .

[13]  D. Dumanović,et al.  Simultaneous determination of N-unsubstituted- and N-substituted nitroazoles and criteria for their identification-I Nitroimidazoles and nitropyrazoles-polarographic determination. , 1973, Talanta.

[14]  D. Sužnjević,et al.  Polarographic and electrochemical studies of some aromatic and heterocyclic nitro compounds, part I: General mechanistic aspects , 1992 .