Toluidine blue modified self-assembled silica gel coated gold electrode as biosensor for NADH

A toluidine blue modified gold electrode was constructed using self-assembled silica gel technique. Firstly, toluidine blue was encapsulated within 3D network of silica self-assembly monolayer on the surface of gold electrode. Secondly, another layer of silica sol was further assembled to protect from leaching of mediator or possible contamination. The electrochemical characteristics of toluidine blue immobilized within self-assembled silica gel were studied in detail. The modified electrode was applied for electrochemical oxidation of NADH with satisfactory results.

[1]  S. Sampath,et al.  Electrochemical oxidation of NADH on sol–gel derived, surface renewable, non-modified and mediator modified composite-carbon electrodes , 1998 .

[2]  John P. Hart,et al.  Chemically modified, carbon-based electrodes and their application as electrochemical sensors for the analysis of biologically important compounds. A review , 1992 .

[3]  Ovadia Lev,et al.  Sol-Gel Derived Composite Ceramic Carbon Electrodes , 2001 .

[4]  T. Wink,et al.  Self-assembled monolayers for biosensors. , 1997, The Analyst.

[5]  S. Saidman,et al.  Electrocatalytic detection of NADH and glycerol by NAD(+)-modified carbon electrodes. , 2000, Analytical chemistry.

[6]  J. Justin Gooding,et al.  The application of alkanethiol self-assembled monolayers to enzyme electrodes , 1999 .

[7]  Tautgirdas Ruzgas,et al.  Electrochemical study of the redox dyes Nile Blue and Toluidine Blue adsorbed on graphite and zirconium phosphate modified graphite , 2000 .

[8]  Bo Xu,et al.  Hydrogen peroxide biosensor with enzyme entrapped within electrodeposited polypyrrole based on mediated sol–gel derived composite carbon electrode , 2001 .

[9]  L. Gorton,et al.  A comparative study of some 3,7-diaminophenoxazine derivatives and related compounds for electrocatalytic oxidation of NADH , 1990 .

[10]  Bruce Dunn,et al.  Sol-gel encapsulation methods for biosensors , 1994 .

[11]  E. Katz,et al.  Surface‐modified gold electrodes for electrocatalytic oxidation of NADH based on the immobilization of phenoxazine and phenothiazine derivatives on self‐assembled monolayers , 1995 .

[12]  Joseph Wang,et al.  Self-Assembled Silica Gel Networks , 1998 .

[13]  E. Katz,et al.  Toluidine blue covalently immobilized onto gold electrode surfaces: An electrocatalytic system for nadh oxidation , 1994 .

[14]  F. Scheller,et al.  Determination of L-phenylalanine based on an NADH-detecting biosensor. , 1998, Analytical Chemistry.

[15]  Joseph Wang,et al.  Sol–gel materials for electrochemical biosensors , 1999 .

[16]  S. Sampath,et al.  Inert metal-modified, composite ceramic-carbon, amperometric biosensors:  renewable, controlled reactive layer. , 1996, Analytical chemistry.

[17]  Iqbal Gill,et al.  Bio-doped Nanocomposite Polymers: Sol-Gel Bioencapsulates , 2001 .

[18]  Alain Walcarius,et al.  Electrochemical Applications of Silica-Based Organic−Inorganic Hybrid Materials , 2001 .

[19]  S. Sampath,et al.  A binderless, bulk-modified, renewable surface amperometric sensor for NADH and ethanol. , 2000, Analytical chemistry.

[20]  N. K. Chaki,et al.  Self-assembled monolayers as a tunable platform for biosensor applications. , 2002, Biosensors & bioelectronics.

[21]  O. Lev,et al.  Sol-Gel-Derived Ceramic-Carbon Composite Electrodes: Introduction and Scope of Applications , 1994 .

[22]  G. Zhu,et al.  Bienzymatic amperometric biosensor for glucose based on polypyrrole/ceramic carbon as electrode material , 2002 .