Expression of Neural Antigens in Normal Xenopus Embryos and Induced Explants

Monoclonal antibodies were raised against neural tissues of Xenopus larvae. Three monoclonal antibodies, named NEU‐1, NEU‐3, and NEU‐4, were specific for neural tissue and first bound to neural cells at stage 25 after neural tube formation (NEU‐1 and NEU‐3) or at stage 31 (NEU‐4). These antibodies bound to differentiating neural cells, but not to germinal neuroepithelial cells. NEU‐1 and NEU‐3 recognized antigens in cell bodies as well as neural fibers of neural cells, and these antigens were distributed throughout the central nervous system. NEU‐4 bound to antigens in granular materials in neural cells, and these antigens were present in head and trunk regions but not in the tail region.

[1]  A. Yamashita,et al.  The expression of epidermal antigens in Xenopus laevis. , 1988, Development.

[2]  A. Durston,et al.  Protein kinase C mediates neural induction in Xenopus laevis , 1988, Nature.

[3]  C. Sharpe.  Developmental expression of a neurofilament-M and two vimentin-like genes in Xenopus laevis. , 1988, Development.

[4]  M. Asada‐Kubota.  A monoclonal antibody specific for an epidermal cell antigen of Xenopus laevis: electron microscopic observations using a gold-labeling method. , 1988, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[5]  S. Mitani,et al.  Monoclonal antibodies against larval nervous system of Xenopus laevis: Their specificities and application to analysis of neural development , 1988, Neuroscience.

[6]  H. Woodland,et al.  The development of an assay to detect mRNAs that affect early development. , 1987, Development.

[7]  E. Jones,et al.  The development of animal cap cells in Xenopus: a measure of the start of animal cap competence to form mesoderm , 1987 .

[8]  G. Edelman,et al.  Expression sequences and distribution of two primary cell adhesion molecules during embryonic development of Xenopus laevis , 1987, The Journal of cell biology.

[9]  J. Gurdon,et al.  A homeobox-containing marker of posterior neural differentiation shows the importance of predetermination in neural induction , 1987, Cell.

[10]  J. Slack,et al.  Regional specification within the mesoderm of early embryos of Xenopus laevis. , 1987, Development.

[11]  G. Malacinski,et al.  Localization of Xenopus homoeo-box gene transcripts during embryogenesis and in the adult nervous system. , 1987, Developmental biology.

[12]  I. Dawid,et al.  Cell-type-specific expression of epidermal cytokeratin genes during gastrulation of Xenopus laevis. , 1987, Genes & development.

[13]  D. Melton,et al.  Expression of Xenopus N-CAM RNA in ectoderm is an early response to neural induction. , 1987, Development.

[14]  J. Gurdon.  Embryonic induction--molecular prospects. , 1987, Development.

[15]  M. Jacobson,et al.  Neural cell adhesion molecule expression in Xenopus embryos. , 1987, Developmental biology.

[16]  B. Anderton,et al.  The appearance and distribution of intermediate filament proteins during differentiation of the central nervous system, skin and notochord of Xenopus laevis. , 1986, Journal of embryology and experimental morphology.

[17]  M. Jacobson,et al.  Induction of neural cell adhesion molecule (NCAM) in Xenopus embryos. , 1986, Developmental biology.

[18]  N. K. Wessells,et al.  Expression of an epidermal antigen used to study tissue induction in the early Xenopus laevis embryo. , 1986, Science.

[19]  E. Jones,et al.  Development of the ectoderm in Xenopus: Tissue specification and the role of cell association and division , 1986, Cell.

[20]  J. Gurdon,et al.  Activation of muscle-specific actin genes in xenopus development by an induction between animal and vegetal cells of a blastula , 1985, Cell.

[21]  J. Slack.  Peanut lectin receptors in the early amphibian embryo: Regional markers for the study of embryonic induction , 1985, Cell.

[22]  G. Malacinski,et al.  Axial structure development in ultraviolet-irradiated (notochord-defective) amphibian embryos. , 1981, Developmental biology.

[23]  R. R. Bensley,et al.  Embryonic Development and Induction , 1938, The Yale Journal of Biology and Medicine.

[24]  J. M. W. Slack,et al.  From Egg to Embryo , 1983 .

[25]  O. Nakamura,et al.  Formation of the Organizer from Combinations of Presumptive Ectoderm and Endoderm. I , 1971 .