A diapause associated protein of the pink bollworm Pectinophora gossypiella Saunders.

A diapause associated protein was electrophoretically isolated from the hemolymph of diapausing last instar larvae of the pink bollworm Pectinophora gossypiella. This protein (M(r) approximately 490,000, glycolipoprotein) was given the name Pectinophora diapause protein (PDP). It is composed of one subunit (M(r) 103,000). The concentration of PDP increased dramatically in the hemolymph of diapausing larvae from 17.4% in prediapause (PD) phase to 29.2% in early diapause (ED) phase reaching a level of 38.6% in larval hemolymph of middiapause (MD) phase. The concentrations of total proteins in the hemolymph of active feeding (A), PD, ED, and MD larvae were 69.8, 106,6, 113.3, and 118 mg/ml, respectively, while those in the fat body of the same larvae were 7.1, 7.4, 8.8, and 4.5 mg/g, respectively. In Pectinophora a drop in the concentration of fat body proteins coincided with a corresponding increase in hemolymph proteins, which suggests an active release of protein from the fat body into the hemolymph during the development of diapause. A partial amino acid sequence of pectinophorin showed the first 15 amino acids starting from the amino terminus of the peptide chain: N-ALA-LYS-THR-ILEU-VAL-GLU-ASN-MET-PRO-PRO-THR-PRO-LEU-ASN-ALA-C.

[1]  Koichi Suzuki,et al.  Specific protein related to adult diapause in the leaf beetle, Gastrophysa atrocyanea , 1990 .

[2]  J. H. Law,et al.  Insect Haemolymph Proteins , 1990 .

[3]  G. C. Unnithan,et al.  A high molecular weight diapause-associated protein from the stem-borer Busseola fusca: Purification and properties , 1989 .

[4]  G. Chippendale,et al.  Purification and properties of a protein that accumulates in the fat body of pre-diapausing larvae of the southwestern corn borer, Diatraea grandiosella , 1984 .

[5]  J. Woodring.  The effects of starving versus fasting on blood composition in larval house crickets , 1984 .

[6]  L. Hood,et al.  Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. , 1983, Methods in enzymology.

[7]  A. Loof,et al.  A re-examination of the protein pattern of the hemolymph of leptinotarsa decemlineata, with special reference to vitellogenins and diapause proteins , 1982 .

[8]  S. Turunen,et al.  Protein release from the larval fat body of the southwestern corn borer, Diatraea grandiosella: An in vitro study , 1980 .

[9]  S. Turunen,et al.  Proteins of the fat body of non-diapausing and diapausing larvae of the southwestern corn borer, Diatraea grandiosella: Effect of juvenile hormone , 1980 .

[10]  John L. Brown.  Haemolymph protein reserves of diapausing and nondiapausing codling moth larvae, Cydia pomonella (L) (Lepidoptera: Tortricidae) , 1980 .

[11]  J. J. Brown,et al.  Juvenile hormone and a protein associated with the larval diapause of the southwestern corn borer, Diatraea grandiosella , 1978 .

[12]  G. R. Wyatt,et al.  Insect plasma proteins. , 1978, Annual review of biochemistry.

[13]  G. Chippendale.  HORMONAL REGULATION OF LARVAL DIAPAUSE , 1977 .

[14]  G. Chippendale,et al.  Horomonal control of larval diapause and metamorphosis of the southwestern corn borer Diatraea grandiosella. , 1976, The Journal of experimental biology.

[15]  A. King.  PHOTOPERIODIC INDUCTION AND INHERITANCE OF DIAPAUSE IN PIONEA FORFICALIS (LEPIDOPTERA: PYRALIDAE) , 1974 .

[16]  M. Florkin,et al.  Chapter 6 – HEMOLYMPH: COMPOSITION , 1974 .

[17]  G. Chippendale.  DIAPAUSE OF THE SOUTHWESTERN CORN BORER, DIATRAEA GRANDIOSELLA: UTILIZATION OF FAT BODY AND HAEMOLYMPH RESERVES 1 , 1973 .

[18]  Z. Smilowitz.  Hemolymph Proteins in Developing Cabbage Looper Larvae and Pupae , 1971 .

[19]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[20]  E. Munn,et al.  The synthesis in vitro of the protein calliphorin by fat body from the larva of the blowfly, Calliphora erythrocephala , 1969 .

[21]  G. Chippendale,et al.  Relationship between the proteins of the haemolymph and fat body during development of Pieris brassicae , 1969 .

[22]  R. Schoental.  Methylation of nucleic acids by N[14C]-methyl-N-nitrosourethane in vitro and in vivo. , 1967, The Biochemical journal.

[23]  S. D. Beck,et al.  Haemolymph proteins of Ostrinia nubilalis during diapause and prepupal differentiation , 1966 .

[24]  P. Adkisson.  Internal clocks and insect diapause. , 1966, Science.

[25]  G. Price,et al.  The electrophoretic separation of proteins isolated from the larva of the blowfly, Calliphora erythrocephala. , 1966, Journal of insect physiology.

[26]  A. Hudson.  Proteins in the haemolymph and other tissues of the developing tomato hornworm, Protoparce quinquemaculata Haworth. , 1966, Canadian journal of zoology.

[27]  J. Clarke.  SIMPLIFIED “DISC” (POLYACRYLAMIDE GEL) ELECTROPHORESIS * , 1964, Annals of the New York Academy of Sciences.

[28]  B. Davis.  DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS * , 1964, Annals of the New York Academy of Sciences.

[29]  M. Rockstein.  The physiology of Insecta , 1964 .

[30]  D. Gilmour.  The Biochemistry Of Insects , 1961 .

[31]  G. R. Wyatt,et al.  THE CHEMISTRY OF INSECT HEMOLYMPH , 1956, The Journal of General Physiology.

[32]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.