Protein profiles of dengue-infected Aedes aegypti (L)

The protein profiles of DENV-2 and DENV-4 viruses-infected adult Aedes aegypti were analysed using Coomassie-stained SDS-PAGE samples. For this study, adult female mosquitoes were infected orally with either DENV-2 or DENV-4 virus using spiked human blood samples. The infections in the individual mosquitoes were confirmed using the reverse transcription-polymerase chain reaction (RT-PCR). From the SDS-PAGE analyses, a total of 11 proteins with molecular weights of 22 kDa to greater than 181.8 kDa were identified in DENV-2 and DENV-4-infected mosquito homogenates. Three proteins (Mr 49, 64 and 200 kDa) from DENV-2-infected mosquitoes and five proteins (Mr 49, 50, 60, 135 and 200 kDa) from DENV-4-infected mosquitoes were identified at higher concentrations than in uninfected controls. Further work is now needed to specifically identify these proteins and study their biological roles in DENV infections.

[1]  T. Livdahl,et al.  Aedes aegypti and Aedes albopictus in Bermuda: extinction, invasion, invasion and extinction , 2010, Biological Invasions.

[2]  H. L. Lee,et al.  The effect of extrinsic incubation temperature on development of dengue serotype 2 and 4 viruses in Aedes aegypti (L.). , 2009, The Southeast Asian journal of tropical medicine and public health.

[3]  H. L. Lee,et al.  Protein synthesized by dengue infected Aedes aegypti and Aedes albopictus. , 2005, Tropical biomedicine.

[4]  R. Campos,et al.  Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina. , 2005, Revista latinoamericana de microbiologia.

[5]  H. Chee,et al.  Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry , 2004 .

[6]  H. Chee,et al.  Identification of a 48kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry. , 2004, Biochemical and biophysical research communications.

[7]  A. Nisalak,et al.  High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. , 2002, Journal of Infectious Diseases.

[8]  V. Deubel,et al.  Enzyme-Linked Immunosorbent Assay Specific to Dengue Virus Type 1 Nonstructural Protein NS1 Reveals Circulation of the Antigen in the Blood during the Acute Phase of Disease in Patients Experiencing Primary or Secondary Infections , 2002, Journal of Clinical Microbiology.

[9]  Yi-Ling Lin,et al.  High‐level expression of recombinant dengue viral NS‐1 protein and its potential use as a diagnostic antigen , 2001, Journal of medical virology.

[10]  M. Shahabuddin,et al.  Localization of Midgut-Specific Protein Antigens from Aedes aegypti (Diptera: Culicidae) Using Monoclonal Antibodies , 2001, Journal of medical entomology.

[11]  Laura D. Kramer,et al.  Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single-Tube Multiplex Reverse Transcriptase PCR , 1998, Journal of Clinical Microbiology.

[12]  S. Lam-Phua,et al.  Monitoring of dengue viruses in field-caught Aedes aegypti and Aedes albopictus mosquitoes by a type-specific polymerase chain reaction and cycle sequencing. , 1998, The American journal of tropical medicine and hygiene.

[13]  F. Kafatos,et al.  Plasmodium activates the innate immune response of Anopheles gambiae mosquitoes , 1997, The EMBO journal.

[14]  A. Falconar,et al.  The dengue virus nonstructural-1 protein (NS1) generatesantibodies to common epitopes on human blood clotting,integrin/adhesin proteins and binds to humanendothelial cells: potential implications in haemorrhagic fever pathogenesis , 1997, Archives of Virology.

[15]  S. Lam.  Two Decades of Dengue in Malaysia , 1993 .

[16]  P. Tauil.  [Aedes aegypti and Aedes albopictus in Brazil]. , 1989, Revista da Sociedade Brasileira de Medicina Tropical.

[17]  R. Wirtz,et al.  Reconstituted collagen sausage casings for the blood feeding of mosquitoes. , 1980 .

[18]  L. C. Rutledge,et al.  Studies on the Feeding Response of Mosquitoes to Nutritive Solutions in a New Membrane Feeder. , 1964 .

[19]  F M Skae,et al.  Dengue Fever in Penang , 1902, British medical journal.