OBJECTIVE
To establish HPLC fingerprint for the quality control of processed Rhizoma Atractylodis Macrocephalae (PRAM).
METHODS
14 batches of PRAM were collected from different places and were analyzed with the developed HPLC fingerprints method. The HPLC separation was performed on a Kromasil C18 analytical column (250 mm x 4.6 mm, 5 microm), and gradient elution was performed by mobile phase containing acetonitrile and water. The flow rate was 1.0 mL/min and the detection wavelength was at 242 nm. The temperature of column was 25 degrees C.
RESULTS
Sixteen mutual peaks were selected in chromatography. Among the obtained fingerprints, the most of the detected peaks were separated effectively. The methodological evaluation showed that the method had a good repeatability.
CONCLUSION
The RSD of relative retention time of mutual peaks which existed in all samples was less than 1.1%. The results of peak areas were in accordance with the request of fingerprint. The established fingerprint can be used for the quality control and species identifying of PRAM.