True digestibility of phosphorus in canola and soybean meals for growing pigs: influence of microbial phytase.

Two studies with growing pigs were conducted using the regression analysis technique to estimate the true digestibility of P (TPD) in canola (CM) and soybean (SBM) meals, and quantify the effect of microbial phytase on TPD of CM and SBM. In each study, 48 (Exp. 1) or 36 (Exp. 2) 17-kg barrows were assigned to 6 dietary treatments arranged in a 3 x 2 factorial of 3 graded levels of CM (Exp. 1) or SBM (Exp. 2) at 132, 264, or 396 g/kg, and 2 levels of phytase at 0 or 1,000 units/kg. The total collection method was used to calculate P digestibilities. In Exp. 1, phytase supplementation increased (P < 0.01) the apparent total tract digestibility of P in CM with values ranging from 26 to 33% without phytase and from 57 to 62% with supplemental phytase. Regression of digested P against dietary P intake resulted in a decreased (P < 0.05) TPD estimate of 34.3% for the diet without phytase than the 61.4% TPD estimate for diet with added phytase. In Exp. 2, phytase supplementation improved (P < 0.05) apparent total tract P digestibility for SBM with values ranging from 34.3 to 38.6% without phytase and from 68 to 71.2% with supplemental phytase. True P digestibility estimate for SBM without phytase at 40.9% was different (P < 0.05) from that with added phytase at 70.8%. These results indicate a TPD of 34 or 41% in growing pigs fed CM or SBM and demonstrate that the addition of microbial phytase at 1,000 units/kg improves true digestibility of P in CM and SBM by 78 and 73%, respectively.

[1]  H. Stein,et al.  Novel procedure for estimating endogenous losses and measurement of apparent and true digestibility of phosphorus by growing pigs. , 2006, Journal of animal science.

[2]  R. Dilger,et al.  Estimation of true phosphorus digestibility and endogenous phosphorus loss in growing pigs fed conventional and low-phytate soybean meals. , 2006, Journal of animal science.

[3]  G. Cromwell,et al.  Estimation of endogenous phosphorus loss in growing and finishing pigs fed semi-purified diets. , 2006, Journal of animal science.

[4]  R. Thaler,et al.  Calcium, phosphorus, and amino acid digestibility in low-phytate corn, normal corn, and soybean meal by growing pigs. , 2005, Journal of animal science.

[5]  O. Adeola,et al.  The efficacy of an Escherichia coli-derived phytase preparation. , 2004, Journal of animal science.

[6]  M. Fan,et al.  Determination of true digestive utilization of phosphorus and the endogenous phosphorus outputs associated with soybean meal for growing pigs. , 2003, Journal of animal science.

[7]  W. Sauer,et al.  EFFECT OF PHYTASE SUPPLEMENTATION TO BARLEY-CANOLA MEAL AND BARLEY-SOYBEAN MEAL DIETS ON PHOSPHORUS AND CALCIUM BALANCE IN GROWING PIGS , 2003 .

[8]  B. Joern,et al.  Phytase, high-available-phosphorus corn, and storage effects on phosphorus levels in pig excreta. , 2003, Journal of environmental quality.

[9]  O. Adeola,et al.  Does supplemental dietary microbial phytase improve amino acid utilization? A perspective that it does not , 2003 .

[10]  M. Fan,et al.  Use of the regression analysis technique to determine the true phosphorus digestibility and the endogenous phosphorus output associated with corn in growing pigs. , 2002, The Journal of nutrition.

[11]  M. Fan,et al.  Additivity of apparent ileal and fecal phosphorus digestibility values measured in single feed ingredients for growing-finishing pigs , 2002 .

[12]  T. Rideout,et al.  Novel methodology allows simultaneous measurement of true phosphorus digestibility and the gastrointestinal endogenous phosphorus outputs in studies with pigs. , 2001, The Journal of nutrition.

[13]  D. Ledoux,et al.  In vitro prediction of phosphorus availability in feed ingredients for swine , 1998 .

[14]  J. H. Kim,et al.  Bioavailability of phosphorus in feeds of plant origin for pigs - Review - , 1997 .

[15]  M. McBurney,et al.  Estimation by regression analysis of endogenous amino acid levels in digesta collected from the distal ileum of pigs. , 1995, Journal of animal science.

[16]  A. Engelen,et al.  Simple and rapid determination of phytase activity. , 1994, Journal of AOAC International.

[17]  Simon Staal Nielsen,et al.  Anion-exchange high-performance liquid chromatography with post-column detection for the analysis of phytic acid and other inositol phosphates. , 1993, Journal of chromatography. A.

[18]  D. Baker,et al.  Efficiency of dietary methionine utilization by young pigs. , 1992, The Journal of nutrition.

[19]  P. A. Kemme,et al.  The effect of supplementary Aspergillus niger phytase in diets for pigs on concentration and apparent digestibility of dry matter, total phosphorus, and phytic acid in different sections of the alimentary tract. , 1992, Journal of animal science.

[20]  P. A. Kemme,et al.  Improvement of phosphorus availability by microbial phytase in broilers and pigs , 1990, British Journal of Nutrition.

[21]  W. Horwitz Official Methods of Analysis , 1980 .

[22]  D. J. Finney Statistical Method in Biological Assay , 1966 .

[23]  S. Hodgkinson,et al.  Physiological approaches to determining gut endogenous amino acid flows in the mammal. , 1998, Archiv fur Tierernahrung.

[24]  C. Hof,et al.  Digestibility of phosphorus in protein-rich ingredients for pig diets. , 1997, Archiv fur Tierernahrung.

[25]  V. Raboy Accumulation and Storage of Phosphate and Minerals , 1997 .

[26]  V. Ravindran,et al.  Phytates: occurrence, bioavailability and implications in poultry nutrition , 1995 .

[27]  P. A. Kemme,et al.  4 – PHOSPHORUS AVAILABILITY AND REQUIREMENTS IN PIGS , 1991 .

[28]  G. Cromwell Biological availability of phosphorus for pigs , 1980 .