Ultrasensitive Label-Free Electrochemical Biosensor for MicroRNA-21 Detection based on 2 ′-O-methyl modified DNAzyme and Duplex-Specific Nuclease Assisted Target Recycling

Based on a highly efficient 2'-O-methyl modified G-quadruplex-hemin DNAzyme and duplex-specific nuclease (DSN) assisted target recycling, a novel label-free electrochemical biosensor for microRNA-21 (miR-21) detection is developed here. By employing the strategy, this DNA biosensor can detect as low as 8 aM miR-21 and exhibits high discrimination ability even against a single-base mismatch.

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