Zone Electrophoresis in a Starch Supporting Medium

Summary 1. A procedure was described for the electrophoretic separation and isolation of materials employing various types of supporting media. 2. Starch proved particularly useful because of its low adsorption of proteins and peptides in aqueous buffers. 3. A comparison was made of the electroosmotic flow in various media under similar conditions. 4. The separation of 1-4 cc of serum with isolation of the components could be carried out employing the starch system. 5. The α and β-lipoproteins of serum could be determined quantitatively by phospholipid and cholesterol analyses of the serum fractions.