Polymeric cryogels as promising materials of biotechnological interest.

Cryogels are gel matrices that are formed in moderately frozen solutions of monomeric or polymeric precursors. Cryogels typically have interconnected macropores (or supermacropores), allowing unhindered diffusion of solutes of practically any size, as well as mass transport of nano- and even microparticles. The unique structure of cryogels, in combination with their osmotic, chemical and mechanical stability, makes them attractive matrices for chromatography of biological nanoparticles (plasmids, viruses, cell organelles) and even whole cells. Polymeric cryogels are efficient carriers for the immobilization of biomolecules and cells.

[1]  L. Lazzeri,et al.  Enzyme-based bioartificial polymeric materials : The α-amylase-poly(vinyl alcohol) system , 1997 .

[2]  V. Lozinsky.  Cryogels on the basis of natural and synthetic polymers: preparation, properties and application , 2002 .

[3]  D. Thomas,et al.  Immobilization of thylakoids in porous particles and stabilization of the photochemical processes by glutaraldehyde action at subzero temperature , 1979, European journal of applied microbiology and biotechnology.

[4]  V. Lozinsky.  Cryotropic gelation of poly(vinyl alcohol) solutions , 1998 .

[5]  R. Zhuo,et al.  Preparation of fast responsive, temperature-sensitive poly(N-isopropylacrylamide) hydrogel , 1999 .

[6]  Makoto Suzuki,et al.  An approach to artificial muscle using polymer gels formed by micro-phase separation , 1993 .

[7]  M. Kumakura.  Preparation of immobilized cellulase beads and their application to hydrolysis of cellulosic materials , 1997 .

[8]  R. Freitag,et al.  An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography , 2000 .

[9]  V. S. Parmar,et al.  Enantioselective hydrolysis of a Schiff's base of D,L-Phenylalanine ethyl ester in water-poor media via the reaction catalyzed with α-chymotrypsin immobilized on hydrophilic macroporous gel support , 2000 .

[10]  Kouichi Sutani,et al.  The synthesis and the electric-responsiveness of hydrogels entrapping natural polyelectrolyte , 2001 .

[11]  Jyh-Ping Chen.  Enzymes immobilized in smart hydrogels , 2001 .

[12]  V. S. Parmar,et al.  Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media , 2000, Biotechnology Letters.

[13]  M. Okazaki,et al.  Development of poly(vinyl alcohol) hydrogel for waste water cleaning. II. Treatment of N,N‐dimethylformamide in waste water with poly(vinyl alcohol) gel with immobilized microorganisms , 1995 .

[14]  D. Josić,et al.  Monoliths as stationary phases for separation of proteins and polynucleotides and enzymatic conversion. , 2001, Journal of chromatography. B, Biomedical sciences and applications.

[15]  N. Barbani,et al.  Effect of chitosan and dextran on the properties of poly(vinyl alcohol) hydrogels , 1999, Journal of materials science. Materials in medicine.

[16]  C. R. Ethier,et al.  Measurement of Gd-DTPA diffusion through PVA hydrogel using a novel magnetic resonance imaging method. , 1999, Biotechnology and bioengineering.

[17]  A. Rodrigues.  Permeable packings and perfusion chromatography in protein separation. , 1997, Journal of chromatography. B, Biomedical sciences and applications.

[18]  Nikolaos A. Peppas,et al.  Structure and Applications of Poly(vinyl alcohol) Hydrogels Produced by Conventional Crosslinking or by Freezing/Thawing Methods , 2000 .

[19]  E. Kokufuta,et al.  A hydrogel capable of facilitating polymer diffusion through the gel porosity and its application in enzyme immobilization , 1992 .

[20]  B. Mattiasson,et al.  The potential of polymeric cryogels in bioseparation , 2001, Bioseparation.

[21]  M. Kumakura.  Preparation method of porous polymer materials by radiation technique and its application , 2001 .

[22]  F. Plieva,et al.  Poly(vinyl alcohol) cryogels employed as matrices for cell immobilization. 3. Overview of recent research and developments , 1998 .

[23]  B Mattiasson,et al.  'Smart' polymers and what they could do in biotechnology and medicine. , 1999, Trends in biotechnology.

[24]  B. Mattiasson,et al.  Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent. , 2003, Journal of chromatography. A.

[25]  昌生 南部.  ゴム状高含水ポリ (ビニルアルコール) ゲル , 1990 .

[26]  B. Mattiasson,et al.  Chromatography of microbial cells using continuous supermacroporous affinity and ion-exchange columns. , 2002, Journal of chromatography. A.

[27]  H. Chase,et al.  Purification of proteins by adsorption chromatography in expanded beds. , 1994, Trends in biotechnology.

[28]  G. Quash,et al.  Purification of measles virus by affinity chromatography and by ultracentrifugation: a comparative study. , 1991, Journal of Virological Methods.

[29]  K. Vorlop,et al.  The Jet Cutting Method as a new immobilization technique , 1998 .

[30]  B. Mattiasson,et al.  Smart polymers for bioseparation and bioprocessing , 2002 .

[31]  S. Downes,et al.  Blends of synthetic and natural polymers as drug delivery systems for growth hormone. , 1995, Biomaterials.

[32]  Hee-Sung Park,et al.  Immobilization of fungus Aspergillus sp. by a novel cryogel technique for production of extracellular hydrolytic enzymes , 2000 .

[33]  F. Plieva,et al.  Peptide synthesis in organic media with subtilisin 72 immobilized on poly(vinyl alcohol)-cryogel carrier. , 2001, Bioorganic & medicinal chemistry letters.

[34]  Ian W. Hamley,et al.  Rapid swelling and deswelling of thermoreversible hydrophobically modified poly(N-isopropylacrylamide) hydrogels prepared by freezing polymerisation , 2002 .

[35]  I. Kaetsu,et al.  Radiation synthesis of polymeric materials for biomedical and biochemical applications , 1993 .