Engineering cell shape and function.

An elastomeric stamp, containing defined features on the micrometer scale, was used to imprint gold surfaces with specific patterns of self-assembled monolayers of alkanethiols and, thereby, to create islands of defined shape and size that support extracellular matrix protein adsorption and cell attachment. Through this technique, it was possible to place cells in predetermined locations and arrays, separated by defined distances, and to dictate their shape. Limiting the degree of cell extension provided control over cell growth and protein secretion. This method is experimentally simple and highly adaptable. It should be useful for applications in biotechnology that require analysis of individual cells cultured at high density or repeated access to cells placed in specified locations.

[1]  G. Whitesides,et al.  Fabrication and imaging of two-dimensional patterns of proteins adsorbed on self-assembled monolayers by scanning electron microscopy , 1993 .

[2]  George M. Whitesides,et al.  Scanning electron microscopy can form images of patterns in self-assembled monolayers , 1993 .

[3]  R Langer,et al.  Switching from differentiation to growth in hepatocytes: Control by extracellular matrix , 1992, Journal of cellular physiology.

[4]  G. Whitesides,et al.  Self-assembled organic monolayers: model systems for studying adsorption of proteins at surfaces , 1991, Science.

[5]  F. Grinnell,et al.  Stress relaxation of contracted collagen gels: disruption of actin filament bundles, release of cell surface fibronectin, and down-regulation of DNA and protein synthesis. , 1991, Experimental cell research.

[6]  D E Ingber,et al.  Fibronectin controls capillary endothelial cell growth by modulating cell shape. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[7]  P. Riddle,et al.  Narrow linear strips of adhesive substratum are powerful inducers of both growth and total focal contact area. , 1990, Journal of cell science.

[8]  J. Caron,et al.  Induction of albumin gene transcription in hepatocytes by extracellular matrix proteins , 1990, Molecular and cellular biology.

[9]  D E Ingber,et al.  Mechanochemical switching between growth and differentiation during fibroblast growth factor-stimulated angiogenesis in vitro: role of extracellular matrix , 1989, The Journal of cell biology.

[10]  A. Ben-Ze'ev,et al.  Cell-cell and cell-matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepatocytes. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Maher,et al.  Support of cultured hepatocytes by a laminin-rich gel. Evidence for a functionally significant subendothelial matrix in normal rat liver. , 1987, The Journal of clinical investigation.

[12]  M J Bissell,et al.  Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[13]  C. O'neill,et al.  Evidence for two distinct mechanisms of anchorage stimulation in freshly explanted and 3T3 Swiss mouse fibroblasts , 1986, Cell.

[14]  D. Kabat,et al.  Cell anchorage determines whether mammary tumor virus glycoproteins are processed for plasma membranes or secretion , 1985, The Journal of cell biology.

[15]  L. Stolt,et al.  Proliferation control in cloned normal and malignant human cells. , 1980, Experimental cell research.

[16]  P. Riddle,et al.  The relation between surface area and anchorage dependence of growth in hamster and mouse fibroblasts , 1979, Cell.

[17]  D. Gospodarowicz,et al.  Determination of cellular shape by the extracellular matrix and its correlation with the control of cellular growth. , 1978, Cancer research.

[18]  J. Folkman,et al.  Role of cell shape in growth control , 1978, Nature.

[19]  B. Westermark,et al.  Growth control in miniclones of human glial cells. , 1978, Experimental cell research.

[20]  A. Harris,et al.  Behavior of cultured cells on substrata of variable adhesiveness. , 1973, Experimental cell research.

[21]  S. Carter,et al.  Haptotaxis and the Mechanism of Cell Motility , 1967, Nature.