论文信息 - in‐vitro evolution

in‐vitro evolution

The complexity of the biological world has arisen through a process of evolution. The study of that process is important in order to understand the continuing evolution of life on Earth, as well as to harness evolution to create new biological tools for human applications. In vitro evolution is a laboratory re-creation of the natural process that allows scientists to watch systems evolve in real time and to control the selective pressures to which molecules and organisms are forced to adapt. In vitro evolution has been used to study the evolution of organisms like bacteria and yeast, as well as biological macromolecules like nucleic acids and proteins. Given the complexity of biological systems, laboratory evolution may be the best way to create new biological machines to solve human problems from renewable energy to cancer.

J. Stapleton | J. A. Stapleton

[1] Gerald F. Joyce,et al. Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA , 1990, Nature.

[2] R. Dorit,et al. Protein cofactor-dependent acquisition of novel catalytic activity by the RNase P ribonucleoprotein of E. coli. , 2001, Journal of molecular biology.

[3] D. Mills,et al. The synthesis of a self-propagating and infectious nucleic acid with a purified enzyme. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[4] F. Arnold,et al. Directed evolution of enzyme catalysts. , 1997, Trends in biotechnology.

[5] Dan S. Tawfik,et al. Man-made cell-like compartments for molecular evolution , 1998, Nature Biotechnology.

[6] F. Kramer,et al. A replicating RNA molecule suitable for a detailed analysis of extracellular evolution and replication. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[7] P. Holliger,et al. Ribozyme-Catalyzed Transcription of an Active Ribozyme , 2011, Science.

[8] F. Arnold,et al. How enzymes adapt: lessons from directed evolution , 2001, Trends in biochemical sciences.

[9] L. Gold,et al. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[10] J. Szostak,et al. In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.