Hybrid biobattery based on arylated carbon nanotubes and laccase.

Single-walled carbon nanotubes (SWCNT) were covalently modified with anthracene and anthraquinone and used for the construction of cathodes for biocatalytic reduction of dioxygen. The nanotubes with aromatic groups casted onto the electrode increased the working surface of the electrode and enabled efficient direct electron transfer (DET) between the enzyme and the electrode. The aryl groups enter the hydrophobic pocket of the T1 center of laccase responsible for exchanging electrons with the substrate. Glassy carbon electrode covered with arylated SWCNT and coated with a layer of neutralized Nafion containing laccase was found to be a very efficient cathode in the hybrid battery. Zn wire covered with a Nafion film served as the anode. The cell parameters were determined: power density was 2 mW/cm(2) and the open circuit potential was 1.5 V.

[1]  J. Nørskov,et al.  Fuel Cell Science: Theory, Fundamentals, and Biocatalysis , 2010 .

[2]  J. Pinson,et al.  Surface Modification of Conducting Substrates. Existence of Azo Bonds in the Structure of Organic Layers Obtained from Diazonium Salts , 2007 .

[3]  Shaojun Dong,et al.  Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes-chitosan composite. , 2006, Biosensors & bioelectronics.

[4]  Maurizio Prato,et al.  Functionalized carbon nanotubes for probing and modulating molecular functions. , 2010, Chemistry & biology.

[5]  Lihong Hu,et al.  Composite films of lecithin and heme proteins with electrochemical and electrocatalytic activities. , 2007, Journal of colloid and interface science.

[6]  F. Marken,et al.  Hydrophilic carbon nanoparticle-laccase thin film electrode for mediatorless dioxygen reduction SECM activity mapping and application in zinc-dioxygen battery , 2009 .

[7]  Adam Heller,et al.  Potentially implantable miniature batteries , 2006, Analytical and bioanalytical chemistry.

[8]  Chul-Jae Lee,et al.  Study of Substitution Effect of Anthraquinone by SERS Spectroscopy , 2004 .

[9]  R. Flores,et al.  Molecular design of laccase cathode for direct electron transfer in a biofuel cell. , 2011, Biosensors & bioelectronics.

[10]  P. Cinquin,et al.  A Glucose BioFuel Cell Implanted in Rats , 2010, PloS one.

[11]  F. Armstrong,et al.  A stable electrode for high-potential, electrocatalytic O(2) reduction based on rational attachment of a blue copper oxidase to a graphite surface. , 2007, Chemical communications.

[12]  Angel Rubio,et al.  Improved Charge Transfer at Carbon Nanotube Electrodes , 1999 .

[13]  G. Wittstock,et al.  Bioelectrocatalytic Carbon Ceramic Gas Electrode for Reduction of Dioxygen and Its Application in a Zinc–Dioxygen Cell , 2010 .

[14]  F C Walsh,et al.  Recent progress and continuing challenges in bio-fuel cells. Part II: Microbial. , 2010, Biosensors & bioelectronics.

[15]  J. Rogalski,et al.  Pyrene sulfonate functionalised single-walled carbon nanotubes for mediatorless dioxygen bioelectrocatalysis , 2009 .

[16]  Lei Zhang.  Functionalization of single walled carbon nanotubes , 2006 .

[17]  Plamen Atanassov,et al.  Direct Bioelectrocatalysis of PQQ‐Dependent Glucose Dehydrogenase , 2007 .

[18]  Liisa Viikari,et al.  Development of a printable laccase-based biocathode for fuel cell applications , 2008 .

[19]  Chulhwan Park,et al.  Use of bioelectrode containing DNA-wrapped single-walled carbon nanotubes for enzyme-based biofuel cell , 2010 .

[20]  K. Roberts,et al.  Synthesis, characterization, and electrochemical testing of carbon nanotubes derivatized with azobenzene and anthraquinone , 2009 .

[21]  Bin Wang,et al.  Recent development of non-platinum catalysts for oxygen reduction reaction , 2005 .

[22]  G. Tayhas R. Palmore,et al.  Electro-enzymatic reduction of dioxygen to water in the cathode compartment of a biofuel cell , 1999 .

[23]  D. Ivnitski,et al.  High electrocatalytic activity of tethered multicopper oxidase-carbon nanotube conjugates. , 2010, Chemical communications.

[24]  M. Shim,et al.  Functionalization of Carbon Nanotubes for Biocompatibility and Biomolecular Recognition , 2002 .

[25]  T Laurell,et al.  Evaluation of glucose biosensors based on Prussian Blue and lyophilised, crystalline and cross-linked glucose oxidases (CLEC(R)). , 2001, Talanta.

[26]  W. Schuhmann,et al.  ABTS-modified multiwalled carbon nanotubes as an effective mediating system for bioelectrocatalytic reduction of oxygen. , 2008, Analytical chemistry.

[27]  A. Heller.  Miniature biofuel cells , 2004 .

[28]  Feng Gao,et al.  An enzymatic glucose/O2 biofuel cell: Preparation, characterization and performance in serum , 2007 .

[29]  S. Cosnier,et al.  Laccase electrodes based on the combination of single-walled carbon nanotubes and redox layered double hydroxides: Towards the development of biocathode for biofuel cells , 2010 .

[30]  J. Tour,et al.  Dispersion of Functionalized Carbon Nanotubes in Polystyrene , 2002 .

[31]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[32]  J. Rogalski,et al.  Derivatization of single-walled carbon nanotubes with redox mediator for biocatalytic oxygen electrodes. , 2010, Bioelectrochemistry.

[33]  J. Rogalski,et al.  Enzymatic biofuel cell based on electrodes modified with lipid liquid-crystalline cubic phases , 2008 .

[34]  Shelley D. Minteer,et al.  Development of a membraneless ethanol/oxygen biofuel cell , 2006 .

[35]  Stanislaus S. Wong,et al.  Covalent Surface Chemistry of Single‐Walled Carbon Nanotubes , 2005 .

[36]  A. Leonowicz,et al.  Quantitative estimation of laccase forms in some white-rot fungi using syringaldazine as a substrate , 1981 .

[37]  F C Walsh,et al.  Biofuel cells and their development. , 2006, Biosensors & bioelectronics.

[38]  C. Banks,et al.  Oxygen reduction catalysis at anthraquinone centres molecularly wired via carbon nanotubes , 2005 .

[39]  A. Kamińska,et al.  Pyrene-functionalised single-walled carbon nanotubes for mediatorless dioxygen bioelectrocatalysis , 2010 .

[40]  F. Armstrong,et al.  Enzymes as working or inspirational electrocatalysts for fuel cells and electrolysis. , 2008, Chemical reviews.

[41]  J. Rogalski,et al.  Composite Bioelectrodes Based on Lipidic Cubic Phase with Carbon Nanotube Network , 2009 .

[42]  G. Urban,et al.  Fabrication and characterization of buckypaper-based nanostructured electrodes as a novel material for biofuel cell applications. , 2011, Physical chemistry chemical physics : PCCP.

[43]  Adam Heller,et al.  Ionic conduction in Zn3(PO4)2.4H2O enables efficient discharge of the zinc anode in serum. , 2005, Journal of the American Chemical Society.

[44]  Allen J. Bard,et al.  Electrochemical Methods: Fundamentals and Applications , 1980 .

[45]  J. Tour,et al.  Solvent-free functionalization of carbon nanotubes. , 2003, Journal of the American Chemical Society.

[46]  J. Rogalski,et al.  Vertically aligned carbon nanotube film electrodes for bioelectrocatalytic dioxygen reduction , 2011 .

[47]  Richard G Compton,et al.  Carbon nanotubes contain metal impurities which are responsible for the "electrocatalysis" seen at some nanotube-modified electrodes. , 2006, Angewandte Chemie.

[48]  Scott Calabrese Barton,et al.  Enzymatic biofuel cells for implantable and micro-scale devices , 2004 .

[49]  M H Osman,et al.  Recent progress and continuing challenges in bio-fuel cells. Part I: enzymatic cells. , 2011, Biosensors & bioelectronics.

[50]  Plamen Atanassov,et al.  Enzymatic fuel cells: integrating flow-through anode and air-breathing cathode into a membrane-less biofuel cell design. , 2011, Biosensors & bioelectronics.

[51]  P. Bartlett,et al.  Monolayer anthracene and anthraquinone modified electrodes as platforms for Trametes hirsuta laccase immobilisation. , 2010, Physical chemistry chemical physics : PCCP.

[52]  F. Simon,et al.  Raman scattering from nanomaterials encapsulated into single wall carbon nanotubes , 2007 .

[53]  Y. Zheng,et al.  Carbon nanotube-hydroxyapatite nanocomposite: a novel platform for glucose/O2 biofuel cell. , 2009, Biosensors & bioelectronics.

[54]  M. Shim,et al.  Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[55]  J. Rogalski,et al.  Enzymatic electrodes nanostructured with functionalized carbon nanotubes for biofuel cell applications , 2010, Analytical and bioanalytical chemistry.