A putative amino acid transporter determines sensitivity to the two‐peptide bacteriocin plantaricin JK

Lactobacillus plantarum produces a number of antimicrobial peptides (bacteriocins) that mostly target closely related bacteria. Although bacteriocins are important for the ecology of these bacteria, very little is known about how the peptides target sensitive cells. In this work, a putative membrane protein receptor of the two‐peptide bacteriocin plantaricin JK was identified by comparing Illumina sequence reads from plantaricin JK‐resistant mutants to a crude assembly of the sensitive wild‐type Weissella viridescens genome using the polymorphism discovery tool VAAL. Ten resistant mutants harbored altogether seven independent mutations in a gene encoding an APC superfamily protein with 12 transmembrane helices. The APC superfamily transporter thus is likely to serve as a target for plantaricin JK on sensitive cells.

[1]  J. V. van Dijl,et al.  The Phosphoenolpyruvate:Sugar Phosphotransferase System Is Involved in Sensitivity to the Glucosylated Bacteriocin Sublancin , 2015, Antimicrobial Agents and Chemotherapy.

[2]  T. Kristensen,et al.  Sensitivity to the two‐peptide bacteriocin lactococcin G is dependent on UppP, an enzyme involved in cell‐wall synthesis , 2014, Molecular microbiology.

[3]  T. Kristensen,et al.  A Zn-Dependent Metallopeptidase Is Responsible for Sensitivity to LsbB, a Class II Leaderless Bacteriocin of Lactococcus lactis subsp. lactis BGMN1-5 , 2013, Journal of bacteriology.

[4]  R. P. Ross,et al.  Bacteriocins — a viable alternative to antibiotics? , 2012, Nature Reviews Microbiology.

[5]  David T. Jones,et al.  Detecting pore-lining regions in transmembrane protein sequences , 2012, BMC Bioinformatics.

[6]  Sergey I. Nikolenko,et al.  SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing , 2012, J. Comput. Biol..

[7]  D. Brede,et al.  The Maltose ABC Transporter in Lactococcus lactis Facilitates High-Level Sensitivity to the Circular Bacteriocin Garvicin ML , 2012, Antimicrobial Agents and Chemotherapy.

[8]  M. Kjos,et al.  An Extracellular Loop of the Mannose Phosphotransferase System Component IIC Is Responsible for Specific Targeting by Class IIa Bacteriocins , 2010, Journal of bacteriology.

[9]  M. Kjos,et al.  The Abi Proteins and Their Involvement in Bacteriocin Self-Immunity , 2010, Journal of bacteriology.

[10]  G. Fimland,et al.  The Lactococcin G Immunity Protein Recognizes Specific Regions in Both Peptides Constituting the Two-Peptide Bacteriocin Lactococcin G , 2009, Applied and Environmental Microbiology.

[11]  P. Rogne,et al.  Three-dimensional structure of the two-peptide bacteriocin plantaricin JK , 2009, Peptides.

[12]  M. Kjos,et al.  An overview of the mosaic bacteriocin pln loci from Lactobacillus plantarum , 2009, Peptides.

[13]  Steven J. M. Jones,et al.  Abyss: a Parallel Assembler for Short Read Sequence Data Material Supplemental Open Access , 2022 .

[14]  Eric S. Lander,et al.  Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing , 2008, Nature Methods.

[15]  E. Birney,et al.  Velvet: algorithms for de novo short read assembly using de Bruijn graphs. , 2008, Genome research.

[16]  Juliane Schmidt,et al.  Mutational analysis of putative helix-helix interacting GxxxG-motifs and tryptophan residues in the two-peptide bacteriocin lactococcin G. , 2008, Biochemistry.

[17]  D. Torrents,et al.  Functional and Structural Characterization of the First Prokaryotic Member of the L-Amino Acid Transporter (LAT) Family , 2007, Journal of Biological Chemistry.

[18]  G. Fimland,et al.  Analysis of the Two-Peptide Bacteriocins Lactococcin G and Enterocin 1071 by Site-Directed Mutagenesis , 2007, Applied and Environmental Microbiology.

[19]  Steven Salzberg,et al.  Identifying bacterial genes and endosymbiont DNA with Glimmer , 2007, Bioinform..

[20]  D. Diep,et al.  Common mechanisms of target cell recognition and immunity for class II bacteriocins , 2007, Proceedings of the National Academy of Sciences.

[21]  C. Soto,et al.  Bactericidal Activity of both Secreted and Nonsecreted Microcin E492 Requires the Mannose Permease , 2006, Journal of bacteriology.

[22]  S. Knøchel,et al.  High-level resistance to class IIa bacteriocins is associated with one general mechanism in Listeria monocytogenes. , 2002, Microbiology.

[23]  H. Sahl,et al.  Mode of action of modified and unmodified bacteriocins from Gram-positive bacteria. , 2002, Biochimie.

[24]  M H Saier,et al.  The amino acid/polyamine/organocation (APC) superfamily of transporters specific for amino acids, polyamines and organocations. , 2000, Microbiology.

[25]  K. Tamura,et al.  Absence of a Putative Mannose-Specific Phosphotransferase System Enzyme IIAB Component in a Leucocin A-Resistant Strain ofListeria monocytogenes, as Shown by Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis , 2000, Applied and Environmental Microbiology.

[26]  A. Driessen,et al.  Complementary and Overlapping Selectivity of the Two-Peptide Bacteriocins Plantaricin EF and JK , 1999, Journal of bacteriology.

[27]  V. Eijsink,et al.  Membrane-Mimicking Entities Induce Structuring of the Two-Peptide Bacteriocins Plantaricin E/F and Plantaricin J/K , 1999, Journal of bacteriology.

[28]  V. Eijsink,et al.  Antagonistic Activity of Lactobacillus plantarum C11: Two New Two-Peptide Bacteriocins, Plantaricins EF and JK, and the Induction Factor Plantaricin A , 1998, Applied and Environmental Microbiology.

[29]  M. Collins,et al.  Taxonomic studies on some leuconostoc-like organisms from fermented sausages: description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. , 1993, The Journal of applied bacteriology.

[30]  G. Venema,et al.  Organization and nucleotide sequences of two lactococcal bacteriocin operons , 1991, Applied and environmental microbiology.

[31]  S. Salzberg,et al.  FLASH: fast length adjustment of short reads to improve genome assemblies , 2011, Bioinform..

[32]  P. Rogne,et al.  Structure-function relationships of the non-lanthionine-containing peptide (class II) bacteriocins produced by gram-positive bacteria. , 2009, Current pharmaceutical biotechnology.

[33]  M. Riley,et al.  Bacteriocins: evolution, ecology, and application. , 2002, Annual review of microbiology.

[34]  D. Diep,et al.  A bacteriocin-like peptide induces bacteriocin synthesis in Lactobacillus plantarum C11. , 1995, Molecular microbiology.

[35]  M. Caron,et al.  Structure-Function Relationships , 1991 .