Homology Modeling of Glycosyl Hydrolase Family 18 Enzymes and Proteins

Using state-of-the-art homology modeling methods, three-dimensional coordinates for three family 18 glycosyl hydrolases were determined. The structures for Gp39, Brp39, and chitotriosidase were computer determined using the X-ray coordinates from SmChiA. The results of the modeling efforts are assessed, and comparison of the modeled structures to other known family 18 members is made.

[1]  B. Henrissat,et al.  Stereochemistry of Chitin Hydrolysis by a Plant Chitinase/Lysozyme and X-ray Structure of a Complex with Allosamidin , 2001 .

[2]  B. Henrissat,et al.  Structures and mechanisms of glycosyl hydrolases. , 1995, Structure.

[3]  M. Levitt.  Accurate modeling of protein conformation by automatic segment matching. , 1992, Journal of molecular biology.

[4]  C. Jeuniaux,et al.  Chitin biomass and production in the marine environment , 1991 .

[5]  M. Hennig,et al.  The 1.8 A resolution structure of hevamine, a plant chitinase/lysozyme, and analysis of the conserved sequence and structure motifs of glycosyl hydrolase family 18. , 1996, Journal of molecular biology.

[6]  S. Roseman,et al.  Chitin utilization by marine bacteria. Chemotaxis to chitin oligosaccharides by Vibrio furnissii. , 1991, Journal of Biological Chemistry.

[7]  M. van den Berg,et al.  Synthesis, sorting, and processing into distinct isoforms of human macrophage chitotriosidase. , 1997, European journal of biochemistry.

[8]  G J Davies,et al.  Nomenclature for sugar-binding subsites in glycosyl hydrolases. , 1997, The Biochemical journal.

[9]  B Henrissat,et al.  A classification of glycosyl hydrolases based on amino acid sequence similarities. , 1991, The Biochemical journal.

[10]  C. Hollak,et al.  Marked elevation of plasma chitotriosidase activity. A novel hallmark of Gaucher disease. , 1994, The Journal of clinical investigation.

[11]  The FROG PC series: programs for electron-density and model investigations for proteins , 1993 .

[12]  L. R. Peterson.  Short-term memory. , 1966, Scientific American.

[13]  A Bairoch,et al.  Updating the sequence-based classification of glycosyl hydrolases. , 1996, The Biochemical journal.

[14]  J. Kirsch,et al.  Is aspartate 52 essential for catalysis by chicken egg white lysozyme? The role of natural substrate-assisted hydrolysis. , 1996, Biochemistry.

[15]  M. Desouza,et al.  An estrogen-dependent secretory protein, which shares identity with chitinases, is expressed in a temporally and regionally specific manner in the sheep oviduct at the time of fertilization and embryo development. , 1995, Endocrinology.

[16]  K S Wilson,et al.  Crystal structure of a bacterial chitinase at 2.3 A resolution. , 1994, Structure.

[17]  Mark C. Surles,et al.  Sculpting proteins interactively: Continual energy minimization embedded in a graphical modeling system , 1994, Protein science : a publication of the Protein Society.

[18]  A. Muijsers,et al.  Purification and Characterization of Human Chitotriosidase, a Novel Member of the Chitinase Family of Proteins (*) , 1995, The Journal of Biological Chemistry.

[19]  Brian,et al.  Human cartilage gp-39, a major secretory product of articular chondrocytes and synovial cells, is a mammalian member of a chitinase protein family. , 1993, The Journal of biological chemistry.

[20]  M. Backes,et al.  Rat liver chitobiase: purification, properties, and role in the lysosomal degradation of Asn-linked glycoproteins. , 1989, Archives of biochemistry and biophysics.

[21]  A Bairoch,et al.  New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. , 1993, The Biochemical journal.

[22]  P. Leder,et al.  neu and ras initiate murine mammary tumors that share genetic markers generally absent in c-myc and int-2-initiated tumors. , 1994, Oncogene.

[23]  D. Phillips,et al.  The three-dimensional structure of an enzyme molecule. , 1966, Scientific American.

[24]  J. Thornton,et al.  PROCHECK: a program to check the stereochemical quality of protein structures , 1993 .

[25]  S. Withers,et al.  Approaches to labeling and identification of active site residues in glycosidases , 1995, Protein science : a publication of the Protein Society.

[26]  N. Aronson,et al.  Cloning and expression of the cDNA sequence encoding the lysosomal glycosidase di-N-acetylchitobiase. , 1992, The Journal of biological chemistry.