Abstract : By the end of the 90s, Defence Research and Development Canada (DRDC) initiated the investigation of a novel LIDAR concept which open the possibility of collecting all at once the detailed spectral information contained in the return signals. This 3-year project called SINBAHD (Stand-off INtegrated Bioaerosol Active Hyperspectral Detection) aimed at evaluating the capability of using UV LIF with intensified range-gated spectrometry to detect and characterize bioaerosol from stand-off position. Essentially, the LIDAR system monitors atmospheric volumes in which specific spectrally wide fluorescence signal can be generated from inelastic interactions with complex molecules forming the building blocks of most bioaerosols. This LIF signal is collected by the combination of a dispersive element and a range-gated ICCD that limits the spectral information within the selected volume. This technique has showed an important potential of detecting and discriminating different bioaerosol agent simulants in real time. Through the Standoff Biodetection Working Group (SBWG) under the CANUKUS CBR MOU, SINBAHD was invited to participate in the Joint Biological Standoff Detection System (JBSDS) increment II field demonstration trial. The purpose of this international trial, which took place at Dugway Proving Ground (DPG), Utah in June 2005, was to determine the benchmark sensitivity of different technologies to various biological simulants and interferents. SINBAHD demonstrated its high level of performance and the results made it possible to obtain new spectral signatures.