Recent developments in the nomenclature, presence, isolation, detection and clinical impact of extracellular vesicles

The research field of extracellular vesicles (EVs), such as microparticles and exosomes, is growing exponentially. The goal of this review is to provide an overview of recent developments relevant to the readers of the Journal of Thrombosis and Haemostasis. We will discuss nomenclature, the presence of EVs in fluids, methods of isolation and detection, and emerging clinical implications. Although research on EVs has been performed within the ISTH for over a decade, most of the recent research on EVs has been brought together by the International Society on Extracellular Vesicles (ISEV). To achieve an overview of recent developments, the information provided in this review comes not only from publications, but also from latest meetings of the ISEV (April 2015, Washington, DC, USA), the International Society on Advancement of Cytometry (June 2015, Glasgow, UK), and the ISTH (June 2015, Toronto, Canada).

[1]  Alain Brisson,et al.  Fluorescence triggering: A general strategy for enumerating and phenotyping extracellular vesicles by flow cytometry , 2016, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[2]  G. van den Engh,et al.  Prerequisites for the analysis and sorting of extracellular vesicle subpopulations by high‐resolution flow cytometry , 2016, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[3]  Ksenija Kogej,et al.  Size characterization and quantification of exosomes by asymmetrical-flow field-flow fractionation. , 2015, Analytical chemistry.

[4]  Christian Pilarsky,et al.  Glypican-1 identifies cancer exosomes and detects early pancreatic cancer , 2015, Nature.

[5]  L. O’Driscoll.  Expanding on exosomes and ectosomes in cancer. , 2015, The New England journal of medicine.

[6]  Jacco van Rheenen,et al.  In Vivo Imaging Reveals Extracellular Vesicle-Mediated Phenocopying of Metastatic Behavior , 2015, Cell.

[7]  Henrik J Johansson,et al.  Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. , 2015, Nanomedicine : nanotechnology, biology, and medicine.

[8]  P. Altevogt,et al.  Extracellular vesicle-mediated transfer of functional RNA in the tumor microenvironment , 2015, Oncoimmunology.

[9]  R. Nieuwland,et al.  Extracellular vesicles, tissue factor, cancer and thrombosis – discussion themes of the ISEV 2014 Educational Day , 2015, Journal of extracellular vesicles.

[10]  R. Nieuwland,et al.  Handling and storage of human body fluids for analysis of extracellular vesicles , 2015, Journal of extracellular vesicles.

[11]  T. V. van Leeuwen,et al.  Refractive index determination of nanoparticles in suspension using nanoparticle tracking analysis. , 2014, Nano letters.

[12]  Muneesh Tewari,et al.  Quantitative and stoichiometric analysis of the microRNA content of exosomes , 2014, Proceedings of the National Academy of Sciences.

[13]  I. Sargent,et al.  Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing , 2014, Journal of thrombosis and haemostasis : JTH.

[14]  D. Burgess.  Signalling: Vesicle vehicles of genetic information , 2014, Nature reviews genetics.

[15]  P. Altevogt,et al.  Extracellular Vesicle-Mediated Transfer of Genetic Information between the Hematopoietic System and the Brain in Response to Inflammation , 2014, Journal of Neuroimmunology.

[16]  S. Mornet,et al.  Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration , 2014, Journal of thrombosis and haemostasis : JTH.

[17]  S. Chisholm,et al.  Bacterial Vesicles in Marine Ecosystems , 2014, Science.

[18]  Chris Gardiner,et al.  Measurement of refractive index by nanoparticle tracking analysis reveals heterogeneity in extracellular vesicles , 2014, Journal of extracellular vesicles.

[19]  R. Nieuwland,et al.  Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation , 2014, Journal of extracellular vesicles.

[20]  R. Nieuwland,et al.  Single-step isolation of extracellular vesicles by size-exclusion chromatography , 2014, Journal of extracellular vesicles.

[21]  M. Krumrey,et al.  Towards traceable size determination of extracellular vesicles , 2014, Journal of extracellular vesicles.

[22]  Thomas Thum,et al.  Non-coding RNAs in cardiac remodeling and heart failure. , 2013, Circulation research.

[23]  Jasmin Wagner,et al.  Characterization of Levels and Cellular Transfer of Circulating Lipoprotein-Bound MicroRNAs , 2013, Arteriosclerosis, thrombosis, and vascular biology.

[24]  Paul Harrison,et al.  Classification, Functions, and Clinical Relevance of Extracellular Vesicles , 2012, Pharmacological Reviews.

[25]  Willem Stoorvogel,et al.  Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry , 2012, Nature Protocols.

[26]  Paul J. Harrison,et al.  Invisible vesicles swarm within the iceberg , 2012, Journal of thrombosis and haemostasis : JTH.

[27]  M. V. van Gemert,et al.  Single vs. swarm detection of microparticles and exosomes by flow cytometry , 2012, Journal of thrombosis and haemostasis : JTH.

[28]  Winston Patrick Kuo,et al.  Impact of Biofluid Viscosity on Size and Sedimentation Efficiency of the Isolated Microvesicles , 2012, Front. Physio..

[29]  W. Stoorvogel,et al.  Identification of Distinct Populations of Prostasomes That Differentially Express Prostate Stem Cell Antigen, Annexin A1, and GLIPR2 in Humans1 , 2012, Biology of reproduction.

[30]  R Lacroix,et al.  Impact of pre‐analytical parameters on the measurement of circulating microparticles: towards standardization of protocol , 2012, Journal of thrombosis and haemostasis : JTH.

[31]  Pierre-Yves Turpin,et al.  Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy , 2012, Journal of extracellular vesicles.

[32]  Paul J. Harrison,et al.  Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis , 2011, Nanomedicine : nanotechnology, biology, and medicine.

[33]  Hamid Cheshmi.  Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers , 2011 .

[34]  B. Ferry,et al.  Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the assay. , 2011, Thrombosis research.

[35]  R. Nieuwland,et al.  Cell-derived vesicles exposing coagulant tissue factor in saliva. , 2011, Blood.

[36]  Anikó Szalai,et al.  Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. , 2011, Blood.

[37]  K. Vickers,et al.  MicroRNAs are Transported in Plasma and Delivered to Recipient Cells by High-Density Lipoproteins , 2011, Nature Cell Biology.

[38]  T G van Leeuwen,et al.  Optical and non‐optical methods for detection and characterization of microparticles and exosomes , 2010, Journal of thrombosis and haemostasis : JTH.

[39]  R Lacroix,et al.  Standardization of platelet‐derived microparticle enumeration by flow cytometry with calibrated beads: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop , 2010, Journal of thrombosis and haemostasis : JTH.

[40]  Bruce A. Stanton,et al.  Long-Distance Delivery of Bacterial Virulence Factors by Pseudomonas aeruginosa Outer Membrane Vesicles , 2009, PLoS pathogens.

[41]  R. Flaumenhaft,et al.  Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles. , 2009, Blood.

[42]  A. Guha,et al.  Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells , 2008, Nature Cell Biology.

[43]  J. Lötvall,et al.  Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells , 2007, Nature Cell Biology.

[44]  Aled Clayton,et al.  Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids , 2006, Current protocols in cell biology.

[45]  Bart N Lambrecht,et al.  Proteomic analysis of exosomes isolated from human malignant pleural effusions. , 2004, American journal of respiratory cell and molecular biology.

[46]  C. Hack,et al.  Cell-derived Microparticles Circulate in Healthy Humans and Support Low Grade Thrombin Generation , 2001, Thrombosis and Haemostasis.

[47]  T. Beveridge,et al.  Virulence factors are released from Pseudomonas aeruginosa in association with membrane vesicles during normal growth and exposure to gentamicin: a novel mechanism of enzyme secretion , 1995, Journal of bacteriology.

[48]  D. Rickwood,et al.  Iodixanol: a nonionic iso-osmotic centrifugation medium for the formation of self-generated gradients. , 1994, Analytical biochemistry.

[49]  C. E. West,et al.  Separation of plasma lipoproteins by density-gradient ultracentrifugation. , 1975, Analytical biochemistry.

[50]  P. Wolf.  The Nature and Significance of Platelet Products in Human Plasma , 1967, British journal of haematology.

[51]  Kenneth W Witwer,et al.  Circulating microRNA biomarker studies: pitfalls and potential solutions. , 2015, Clinical chemistry.

[52]  A. Stensballe,et al.  Unfiltered beer: a rich source of yeast extracellular vesicles , 2014 .

[53]  T. Moccetti,et al.  Exosomes secreted by human cardiac progenitor cells inhibit cardiomyocyte apoptosis and improve cardiac function after myocardial infarction , 2014 .

[54]  W. Zacharias,et al.  Exosome isolation for proteomic analyses and RNA profiling. , 2011, Methods in molecular biology.