Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology.

Elastin-like polypeptides (ELPs) are artificial polypeptides, derived from Val-Pro-Gly-Xaa-Gly (VPGXG) pentapeptide repeats found in human tropoelastin, that reversibly coacervate above a critical temperature. Genetically encodable ELPs are monodisperse, stimuli responsive, and biocompatible, properties that make them attractive for drug delivery and tissue engineering. The potential of ELPs to self-assemble into nanostructures in response to environmental triggers is another interesting feature of these polypeptides that promises to lead to a host of new applications.

[1]  Hamidreza Ghandehari,et al.  In vitro and in vivo evaluation of recombinant silk-elastinlike hydrogels for cancer gene therapy. , 2004, Journal of controlled release : official journal of the Controlled Release Society.

[2]  J. West,et al.  Overexpression of Lysyl Oxidase to Increase Matrix Crosslinking and Improve Tissue Strength in Dermal Wound Healing , 2006, Annals of Biomedical Engineering.

[3]  E. Chaikof,et al.  Alterations in physical cross-linking modulate mechanical properties of two-phase protein polymer networks. , 2005, Biomacromolecules.

[4]  J. Rodríguez‐Cabello,et al.  Design and bioproduction of a recombinant multi(bio)functional elastin-like protein polymer containing cell adhesion sequences for tissue engineering purposes , 2004, Journal of materials science. Materials in medicine.

[5]  A. Chilkoti,et al.  Self-cleavable stimulus responsive tags for protein purification without chromatography. , 2005, Journal of the American Chemical Society.

[6]  R. Herrero-Vanrell,et al.  Self-assembled particles of an elastin-like polymer as vehicles for controlled drug release. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[7]  Wilfred Chen,et al.  Affinity purification of plasmid DNA by temperature‐triggered precipitation , 2004, Biotechnology and bioengineering.

[8]  Liang Feng,et al.  Simple bioseparations using self-cleaving elastin-like polypeptide tags , 2005, Nature Methods.

[9]  T. M. Parker,et al.  Biocompatibility of the Bioelastic Materials, Poly(GVGVP) and Its γ-Irradiation Cross-Linked Matrix: Summary of Generic Biological Test Results , 1991 .

[10]  A. Chilkoti,et al.  Ultra‐High Expression of a Thermally Responsive Recombinant Fusion Protein in E. coli , 2006, Biotechnology progress.

[11]  Howard G. Schild,et al.  Microcalorimetric detection of lower critical solution temperatures in aqueous polymer solutions , 1990 .

[12]  M. Heskins,et al.  Solution Properties of Poly(N-isopropylacrylamide) , 1968 .

[13]  Ashok Mulchandani,et al.  Temperature-triggered purification of antibodies. , 2005, Biotechnology and bioengineering.

[14]  V. Conticello,et al.  Thermo‐Reversible Self‐Assembly of Nanoparticles Derived from Elastin‐Mimetic Polypeptides , 2000 .

[15]  Wilfred Chen,et al.  Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR , 2004, Applied and Environmental Microbiology.

[16]  Ashutosh Chilkoti,et al.  Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system. , 2002, Biomacromolecules.

[17]  Wilfred Chen,et al.  Thermally triggered purification and immobilization of elastin–OPH fusions , 2003, Biotechnology and bioengineering.

[18]  Elizabeth R. Wright,et al.  Thermoplastic elastomer hydrogels via self-assembly of an elastin-mimetic triblock polypeptide , 2002 .

[19]  A. Chilkoti,et al.  Protein Purification by Fusion with an Environmentally Responsive Elastin‐Like Polypeptide: Effect of Polypeptide Length on the Purification of Thioredoxin , 2001, Biotechnology progress.

[20]  Wilfred Chen,et al.  Tunable Biopolymers for Heavy Metal Removal , 2001 .

[21]  Wilfred Chen,et al.  Genetically engineered elastin-protein A fusion as a universal platform for homogeneous, phase-separation immunoassay. , 2005, Analytical chemistry.

[22]  Wilfred Chen,et al.  Fabrication of antibody arrays using thermally responsive elastin fusion proteins. , 2006, Journal of the American Chemical Society.

[23]  J. West,et al.  Val-ala-pro-gly, an elastin-derived non-integrin ligand: smooth muscle cell adhesion and specificity. , 2003, Journal of Biomedical Materials Research. Part A.

[24]  D. Urry.  Physical Chemistry of Biological Free Energy Transduction As Demonstrated by Elastic Protein-Based Polymers† , 1997 .

[25]  David A Tirrell,et al.  Comparative cell response to artificial extracellular matrix proteins containing the RGD and CS5 cell-binding domains. , 2004, Biomacromolecules.

[26]  C. Luan,et al.  Solvent deuteration enhancement of hydrophobicity: DSC study of the inverse temperature transition of elastin-based polypeptides , 1991 .

[27]  H. Maeda,et al.  A new concept for macromolecular therapeutics in cancer chemotherapy: mechanism of tumoritropic accumulation of proteins and the antitumor agent smancs. , 1986, Cancer research.

[28]  Ashutosh Chilkoti,et al.  Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides. , 2004, Biomacromolecules.

[29]  E. Chaikof,et al.  Viscoelastic and mechanical behavior of recombinant protein elastomers. , 2005, Biomaterials.

[30]  B J Bennion,et al.  Hydrophobic hydration is an important source of elasticity in elastin-based biopolymers. , 2001, Journal of the American Chemical Society.

[31]  Farshid Guilak,et al.  Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide. , 2006, Biomaterials.

[32]  V. Daggett,et al.  The molecular basis for the inverse temperature transition of elastin. , 2001, Journal of molecular biology.

[33]  David A Tirrell,et al.  Cell-binding domain context affects cell behavior on engineered proteins. , 2005, Biomacromolecules.

[34]  A. Chilkoti,et al.  Aqueous two-phase system formation kinetics for elastin-like polypeptides of varying chain length. , 2006, Biomacromolecules.

[35]  Ashutosh Chilkoti,et al.  Targeted drug delivery by thermally responsive polymers. , 2002, Advanced drug delivery reviews.

[36]  A. Chilkoti,et al.  Structural optimization of a "smart" doxorubicin-polypeptide conjugate for thermally targeted delivery to solid tumors. , 2006, Journal of controlled release : official journal of the Controlled Release Society.

[37]  T. M. Parker,et al.  Delineation of electrostatic- and hydrophobic-induced pKa shifts in polypentapeptides : the glutamic acid residue , 1993 .

[38]  D. Gowda,et al.  Reduction-driven polypeptide folding by the ΔTt mechanism , 1992 .

[39]  Hamidreza Ghandehari,et al.  Genetic Engineering of Protein-Based Polymers: Potential in Controlled Drug Delivery , 1998, Pharmaceutical Research.

[40]  David A Tirrell,et al.  Physical properties of artificial extracellular matrix protein films prepared by isocyanate crosslinking. , 2004, Biomaterials.

[41]  A. Chilkoti,et al.  Enhanced uptake of a thermally responsive polypeptide by tumor cells in response to its hyperthermia-mediated phase transition. , 2001, Cancer research.

[42]  E. Chaikof,et al.  Photomediated Solid-State Cross-Linking of an Elastin-Mimetic Recombinant Protein Polymer , 2002 .

[43]  M. Dewhirst,et al.  Drug targeting using thermally responsive polymers and local hyperthermia. , 2001, Journal of controlled release : official journal of the Controlled Release Society.

[44]  Wilfred Chen,et al.  An immunoassay for atrazine using tunable immunosorbent. , 2003, Analytical biochemistry.

[45]  A. Chilkoti,et al.  Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin‐like polypeptide fusion with an oligohistidine fusion , 2004, Protein science : a publication of the Protein Society.

[46]  D. Urry.  Free energy transduction in polypeptides and proteins based on inverse temperature transitions. , 1992, Progress in biophysics and molecular biology.

[47]  T. LaBean,et al.  Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity? , 2004, Protein engineering, design & selection : PEDS.

[48]  E. Landau,et al.  The Hofmeister series: salt and solvent effects on interfacial phenomena , 1997, Quarterly Reviews of Biophysics.

[49]  T. M. Parker,et al.  Temperature of polypeptide inverse temperature transition depends on mean residue hydrophobicity , 1991 .

[50]  Ashutosh Chilkoti,et al.  Purification of recombinant proteins by fusion with thermally-responsive polypeptides , 1999, Nature Biotechnology.

[51]  A. Asthagiri,et al.  Quantitatively distinct requirements for signaling-competent cell spreading on engineered versus natural adhesion ligands. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[52]  H. Ghandehari,et al.  Thermal analysis of water in silk-elastinlike hydrogels by differential scanning calorimetry. , 2004, Biomacromolecules.

[53]  Ashutosh Chilkoti,et al.  Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair. , 2005, Tissue engineering.

[54]  Dan W. Urry,et al.  Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamics , 1988, Journal of protein chemistry.