Improving the blood compatibility of material surfaces via biomolecule-immobilized mussel-inspired coatings.

In this article, we presented a general protocol to prepare biomolecule-immobilized mussel-inspired polydopamine (PDA) coatings to improve the blood compatibility of broad ranges of material surfaces. It needs only a simple immersion of substrates in dopamine solution at alkaline pH to form mussel-inspired PDA coating, and then immersing the PDA coated substrates into biomolecule solution to conjugate biomolecules. XPS, water contact angle analysis, and protein assay confirmed that biomolecules could be successfully coated on several material surfaces, including nylon, cellulose, and polyethersulfone membrane surfaces. For the protein fouling resistance, the bovine serum albumin (BSA) modified surfaces were more effective than the amino acid modified surfaces. And the platelet adhesion on the BSA-modified material surfaces was obviously depressed. These results indicated that the blood compatibility of the surfaces was improved by the biomacromolecule-immobilized mussel-inspired coating which might be considered as a universal coating to modify a wide variety of materials.

[1]  Haeshin Lee,et al.  Facile Conjugation of Biomolecules onto Surfaces via Mussel Adhesive Protein Inspired Coatings , 2009, Advanced materials.

[2]  Abadie,et al.  Chymotrypsin Adsorption on Montmorillonite: Enzymatic Activity and Kinetic FTIR Structural Analysis. , 1999, Journal of colloid and interface science.

[3]  M. Nomizu,et al.  Blood compatible aspects of DNA-modified polysulfone membrane-protein adsorption and platelet adhesion. , 2003, Biomaterials.

[4]  Xiaohua Huang,et al.  Protein adsorption on blood-contact membranes , 2003 .

[5]  Anthony G. Fane,et al.  Fouling mechanisms of membranes during protein ultrafiltration , 1992 .

[6]  J. Waite,et al.  Polyphosphoprotein from the adhesive pads of Mytilus edulis. , 2001, Biochemistry.

[7]  L. Ting,et al.  An evaluation of a polyethersulfone hollow fiber plasma separator by animal experiment. , 2001 .

[8]  D. Huo,et al.  Investigation on clotting and hemolysis characteristics of heparin-immobilized polyether sulfones biomembrane. , 2008, Journal of biomedical materials research. Part A.

[9]  A. Higuchi,et al.  A systematic SPR study of human plasma protein adsorption behavior on the controlled surface packing of self-assembled poly(ethylene oxide) triblock copolymer surfaces. , 2009, Journal of biomedical materials research. Part A.

[10]  Yusuke Arima,et al.  Effects of surface functional groups on protein adsorption and subsequent cell adhesion using self-assembled monolayers , 2007 .

[11]  A. Mochizuki,et al.  Blood compatible aspects of poly(2-methoxyethylacrylate) (PMEA)--relationship between protein adsorption and platelet adhesion on PMEA surface. , 2000, Biomaterials.

[12]  Benjamin G Keselowsky,et al.  Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion. , 2003, Journal of biomedical materials research. Part A.

[13]  D. Selkoe,et al.  Dopamine covalently modifies and functionally inactivates parkin , 2005, Nature Medicine.

[14]  B. Ratner,et al.  Protein adsorption and clotting time of pHEMA hydrogels modified with C18 ligands to adsorb albumin selectively and reversibly. , 2009, Biomaterials.

[15]  Q. Wei,et al.  Preparation, characterization and application of functional polyethersulfone membranes blended with poly (acrylic acid) gels , 2009 .

[16]  A. Ogwu,et al.  Platelet adhesion on silicon modified hydrogenated amorphous carbon films. , 2004, Biomaterials.

[17]  M. Ulbricht,et al.  Ultrafiltration membrane surfaces with grafted polymer 'tentacles': preparation, characterization and application for covalent protein binding. , 1998, Biomaterials.

[18]  K. Ishihara,et al.  Modification of polysulfone with phospholipid polymer for improvement of the blood compatibility. Part 2. Protein adsorption and platelet adhesion. , 1999, Biomaterials.

[19]  Miaoer Yu,et al.  Role of l-3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins , 1999 .

[20]  Pengli Bai,et al.  Modification of polyethersulfone membrane by grafting bovine serum albumin on the surface of polyethersulfone/poly(acrylonitrile-co-acrylic acid) blended membrane , 2009 .

[21]  D. Klee,et al.  High density binding of proteins and peptides to poly(D,L-lactide) grafted with polyacrylic acid. , 2002, Biomaterials.

[22]  M. Houška,et al.  In vitro hemocompatibility of albumin-heparin multilayer coatings on polyethersulfone prepared by the layer-by-layer technique. , 2006, Journal of biomedical materials research. Part A.

[23]  Z. Gu,et al.  BSA-Modified Polyethersulfone Membrane: Preparation, Characterization and Biocompatibility , 2009, Journal of biomaterials science. Polymer edition.

[24]  J. Chen,et al.  Mechanical properties and platelet adhesion behavior of diamond-like carbon films synthesized by pulsed vacuum arc plasma deposition , 2003 .

[25]  J. Waite,et al.  Cross-linking in adhesive quinoproteins: studies with model decapeptides. , 2000, Biochemistry.

[26]  T. Liu,et al.  An evaluation of a polyethersulfone hollow fiber plasma separator by animal experiment. , 2001, Artificial organs.

[27]  C. Werner,et al.  Adsorption-induced conformational changes of proteins onto ceramic particles: differential scanning calorimetry and FTIR analysis. , 2006, Journal of colloid and interface science.

[28]  J. Courtney,et al.  Novel "anchor modification" of polymeric biomaterial surfaces by the utilization of cyclodextrin inclusion complex supramolecules. , 2009, Journal of biomedical materials research. Part A.

[29]  Haeshin Lee,et al.  Mussel-Inspired Surface Chemistry for Multifunctional Coatings , 2007, Science.

[30]  Remko M. Boom,et al.  Microstructures in phase-inversion membranes. Part I. Formation of macrovoids , 1992 .

[31]  Je Young Kim,et al.  Liquid-liquid phase separation in polysulfone/polyethersulfone/N-methyl-2-pyrrolidone/water quaternary system , 1999 .

[32]  L. Parise,et al.  LA Fitzgerald The platelet membrane glycoprotein IIb-IIIa complex , 1988 .

[33]  M. Ulbricht,et al.  Novel photochemical surface functionalization of polysulfone ultrafiltration membranes for covalent immobilization of biomolecules , 1996 .

[34]  H. Park,et al.  The minimum surface fibrinogen concentration necessary for platelet activation on dimethyldichlorosilane-coated glass. , 1991, Journal of biomedical materials research.