Steinernema ashiuense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from Japan.

Steinernema ashiuense sp. n. was collected by a Galleria baiting method from grassland along the riverbank of the Yura river, near the Ashiu Research Forest Station of Kyoto University. The new species is characterised by an infective juvenile body length of 768 (720-800) μm, lateral field with five equally developed ridges (i.e., six lines or incisures), head smooth lacking horn-like structures, excretory pore located at level of middle of pharynx, hyaline portion short (ca one-third of tail length). Males have 50-65 μm long, broad, slightly yellowish spicules; spicule length / spicule width = 4 (3.5-4.4), and two subventral and one subdorsal pair of genital papillae in the postcloacal region. The analysis of ITS-rDNA sequence placed S. ashiuense sp. n. in the 'feltiae-kraussei-oregonense' group in the clade containing S. robustispiculum and S. monticolum. It can be distinguished from these species by morphological characters of the infective juveniles and males.

[1]  K. B. Nguyen,et al.  Steinernema akhursti sp. n. (Nematoda: Steinernematidae) from Yunnan, China. , 2005, Journal of invertebrate pathology.

[2]  D. Sturhan,et al.  Steinernema silvaticum sp. n. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Europe , 2005 .

[3]  D. Sturhan,et al.  Steinernema weiseri n. sp. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Europe , 2003, Systematic Parasitology.

[4]  R. Bedding,et al.  Neoaplectana Steiner, 1929 a junior synonym of Steinernema Travassos, 1927 (Nematoda; Rhabditida) , 1982, Systematic Parasitology.

[5]  Mutsuhiro Yoshida.  Steinernema litorale n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Japan , 2004 .

[6]  M. Moens,et al.  Steinernema jollieti sp n. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from the American midwest , 2004 .

[7]  S. Subbotin,et al.  Phylogenetic relationships within the genus Steinernema (Nematoda: Rhabditida) as inferred from analyses of sequences of the ITS1-5.8S-ITS2 region of rDNA and morphological features , 2004 .

[8]  S. Subbotin,et al.  A new entomopathogenic nematode, Steinernema robustispiculum n. sp. (Rhabditida: Steinernematidae), from Chumomray National Park in Vietnam , 2004, Systematic Parasitology.

[9]  Mutsuhiro Yoshida.  Intraspecific variation in RFLP patterns and morphological studies on Steinernema feltiae and S. kraussei (Rhabditida: Steinernematidae) from Hokkaido, Japan , 2003 .

[10]  S. Subbotin,et al.  Heterorhabditis baujardi sp. n. (Rhabditida: Heterorhabditidae) from Vietnam and morphometric data for H. indica populations , 2003 .

[11]  R. Gaugler,et al.  Taxonomy and systematics. , 2002 .

[12]  K. Nguyen,et al.  Diagnostic and Phylogenetic Utility of the rDNA Internal Transcribed Spacer Sequences of Steinernema. , 2001, Journal of nematology.

[13]  S. Nadler,et al.  Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) Inferred From Ribosomal DNA Sequences and Morphological Characters , 2001, The Journal of parasitology.

[14]  B. Briscoe,et al.  Survey of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Japan , 1998 .

[15]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[16]  S. Stock,et al.  An Entomopathogenic Nematode, Steinernema Monticolum Sp. N. (Rhabditida: Steinernematidae) From Korea With a Key To Other Species , 1997 .

[17]  R. Berry,et al.  Steinernema oregonensis n. sp. ( Rhabditida : Steinernematidae ) from Oregon, USA , 1996 .

[18]  K. B. Nguyen,et al.  Scanning Electron Microscope Studies of Steinernema Glaseri (Nematoda: Steinernematidae) 1 , 1995 .

[19]  K. Nguyen,et al.  Neosteinernema longicurvicauda n. gen., n. sp. (Rhabditida: Steinernematidae), a Parasite of the Termite Reticuldermes flavipes (Koller). , 1994, Journal of nematology.

[20]  D. Wakarchuk,et al.  Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. , 1992 .

[21]  K. Nguyen,et al.  Steinernema scapterisci n. sp. (Rhabditida: Steinernematidae). , 1990, Journal of nematology.

[22]  Y. Mamiya.  Steinernema kushidai n. sp. (Nematoda: Steinernematidae) Associated with scarabaeid beetle larvae from Shizuoka, Japan. , 1988 .

[23]  J. Eisenback.  A comparison of techniques useful for preparing nematodes for scanning electron microscopy. , 1986, Journal of nematology.

[24]  R. Bedding,et al.  A simple technique for the detection o f insect paristic rhabditid nematodes in soil , 1975 .

[25]  J. Seinhorst.  A Rapid Method for the Transfer of Nematodes From Fixative To Anhydrous Glycerin , 1959 .

[26]  G. F. White.  A METHOD FOR OBTAINING INFECTIVE NEMATODE LARVAE FROM CULTURES. , 1927, Science.