Novel silver nanoparticle-enhanced fluorometric determination of trace tetracyclines in aqueous solutions.

Metal-enhanced fluorescence (MEF) has exhibited promise for applications in fluorometric assays. The effects of silver nanoparticles (AgNP) on the fluorescence behaviours of tetracycline hydrochloride (TCH) and chlortetracycline hydrochloride (CTC) in aqueous solutions were investigated. The experimental results demonstrated that the fluorescence intensities of each tetracycline in water solutions were greatly enhanced by AgNP through the MEF effect. In addition, a novel silver nanoparticle-enhanced fluorometric method was established for the direct determination of TCH and CTC in aqueous solutions. Under optimum experimental conditions, the linear dynamic ranges for the determination of TCH and CTC in aqueous solutions varied from 0.10 to 6.0 mg L(-1) and 0.050 to 3.0 mg L(-1) with detection limits of 0.63 µg L(-1) and 0.19 µg L(-1), respectively, and with the relative standard deviation of less than 1.9% (n=9). The experimental recovery results for the determination of TCH and CTC in aqueous solutions ranged from 93-106% and 95-104%, respectively. Compared with the established method without the addition of AgNP, the limits of quantitation of the silver nanoparticle-enhanced fluorometric method were approximately 5-fold lower for TCH and 3-fold lower for CTC. Moreover, the newly established silver nanoparticle-enhanced fluorometric method was successfully applied to the direct determination of TCH and CTC in pharmaceutical preparations.

[1]  Zhuyuan Wang,et al.  Immunoassays Based on Surface-Enhanced Fluorescence using Gap-Plasmon-Tunable Ag Bilayer Nanoparticle Films , 2012, Journal of Fluorescence.

[2]  J. Luong,et al.  Control of the Size and Distribution of Gold Nanoparticles by Unmodified Cyclodextrins , 2003 .

[3]  J. Hayakawa,et al.  Improvement of chemical analysis of antibodies. XXII: Identification of residual tetracyclines in honey by frit FAB/LC/MS using a volatile mobile phase , 1994 .

[4]  Petr Solich,et al.  An overview of analytical methodologies for the determination of antibiotics in environmental waters. , 2009, Analytica chimica acta.

[5]  张勇,et al.  Investigation of the Effect of Nano Silver on Fluorescence Properties of Norfloxacin , 2012 .

[6]  Alberto Escarpa,et al.  Sensing colorimetric approaches based on gold and silver nanoparticles aggregation: chemical creativity behind the assay. A review. , 2012, Analytica chimica acta.

[7]  S. Hansen,et al.  Use of metal complexation in non-aqueous capillary electrophoresis systems for the separation and improved detection of tetracyclines. , 1997, Journal of chromatography. A.

[8]  J. Lakowicz.  Radiative decay engineering: biophysical and biomedical applications. , 2001, Analytical biochemistry.

[9]  T. Loftsson.  Self-assembled cyclodextrin nanoparticles and drug delivery , 2014, Journal of Inclusion Phenomena and Macrocyclic Chemistry.

[10]  Uttam Anand,et al.  Exploring the mechanism of fluorescence quenching in proteins induced by tetracycline. , 2011, The journal of physical chemistry. B.

[11]  K. Sokolov,et al.  Enhancement of molecular fluorescence near the surface of colloidal metal films. , 1998, Analytical chemistry.

[12]  Á. Ríos,et al.  Rapid determination of trace levels of tetracyclines in surface water using a continuous flow manifold coupled to a capillary electrophoresis system , 2004 .

[13]  F. Jamal,et al.  Gold nanoparticle synthesis in microfluidic systems and immobilisation in microreactors designed for the catalysis of fine organic reactions , 2012 .

[14]  N. Leepipatpiboon,et al.  Validation of a liquid chromatography-mass spectrometry multi-residue method for the simultaneous determination of sulfonamides, tetracyclines, and pyrimethamine in milk. , 2007, Journal of chromatography. A.

[15]  C. D. Geddes,et al.  Metal-enhanced fluorescence. , 2013, Physical chemistry chemical physics : PCCP.

[16]  Sébastien Sauvé,et al.  Quantitative performance of liquid chromatography coupled to Q-Exactive high resolution mass spectrometry (HRMS) for the analysis of tetracyclines in a complex matrix. , 2015, Analytica chimica acta.

[17]  A. Krachler,et al.  Bacterial fitness shapes the population dynamics of antibiotic-resistant and -susceptible bacteria in a model of combined antibiotic and anti-virulence treatment , 2014, Journal of theoretical biology.

[18]  M. Wong,et al.  Simultaneous Determination of Oxytetra- cycline, Doxycycline, Tetracycline and Chlortetracycline in Tetracycline Antibiotics by High-Performance Liquid Chromatog- raphy with Fluorescence Detection , 2004 .

[19]  A. Herrera,et al.  Effect of pasteurization and storage on tetracycline levels in honey , 2011, Apidologie.

[20]  M. Hernández-Córdoba,et al.  Liquid chromatography with ultraviolet absorbance detection for the analysis of tetracycline residues in honey. , 2004, Journal of chromatography. A.

[21]  Yingshu Guo,et al.  Silver nanoparticle-based chemiluminescence enhancement for the determination of norfloxacin , 2010 .

[22]  Citra Ariani Edityaningrum,et al.  Molecular Inclusion Complex of Curcumin–β-Cyclodextrin Nanoparticle to Enhance Curcumin Skin Permeability from Hydrophilic Matrix Gel , 2013, AAPS PharmSciTech.

[23]  J. Lakowicz,et al.  Fluorescence Enhancements on Silver Colloid Coated Surfaces , 2004, Journal of Fluorescence.

[24]  C. D. Geddes,et al.  Editorial: Metal-Enhanced Fluorescence , 2002, Journal of Fluorescence.

[25]  J M Pingarrón,et al.  Rapid screening of multiple antibiotic residues in milk using disposable amperometric magnetosensors. , 2014, Analytica chimica acta.

[26]  Yi-Fang Tian,et al.  Determination of five quinolone antibiotic residues in foods by micellar electrokinetic capillary chromatography with quantum dot indirect laser-induced fluorescence , 2014, Analytical and Bioanalytical Chemistry.

[27]  Hong-Ming Lin,et al.  Nanoparticle suspension preparation using the arc spray nanoparticle synthesis system combined with ultrasonic vibration and rotating electrode , 2005 .

[28]  H. Nakazawa,et al.  Simultaneous determination of residual tetracyclines in foods by high-performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry. , 1999, Journal of chromatography. B, Biomedical sciences and applications.