Biotechnology and the red seaweed polysaccharide industry: status, needs and prospects

Significant amounts of marine macroalgal (seaweed) polysaccharides are used in food, pharmaceuticals and other products for human consumption. Thus, the global seaweed polysaccharide industry operates in a highly regulated environment. Genetic manipulation of macroalgae to alter composition or growth characteristics may lead to products that do not fall within the current regulations: research that is readily translatable to industrial application is generally restricted to seaweed cultivation and processing and new applications of the approved polysaccharides. There is a great need, however, for research into the genome structure and metabolic pathways of commercially important marine macroalgae. This precompetitive research may not be immediately applicable to the seaweed polysaccharide industry but is critical for sustaining future commercial growth.

[1]  C. Boyen,et al.  Nucleotide sequence of the cox3 gene from Chondrus crispus: evidence that UGA encodes tryptophan and evolutionary implications. , 1994, Nucleic acids research.

[2]  A. Gibor,et al.  Calluses, cells, and protoplasts in studies towards genetic improvement of seaweeds , 1986 .

[3]  G. Kraft,et al.  Small-subunit rRNA gene sequences from representatives of selected families of the Gigartinales and Rhodymeniales (Rhodophyta). 1. Evidence for the Plocamiales ord.nov. , 1994 .

[4]  M. Wu,et al.  The sequence of the plastid encoded rpl22 protein in marine macroalgae, Gracilaria tenuistipitata. , 1990, Nucleic Acids Research.

[5]  R. Zilinskas Marine biotechnology and developing countries , 1993 .

[6]  C. Boyen,et al.  DNA sequence, structure, and phylogenetic relationship of the mitochondrial small-subunit rRNA from the red alga Chondrus crispus (Gigartinales, Rhodophytes) , 1995, Journal of Molecular Evolution.

[7]  A. Gibor,et al.  A method for RNA isolation from marine macro-algae. , 1988, Analytical biochemistry.

[8]  M. Hein,et al.  Immunotherapeutic potential of antibodies produced in plants. , 1995, Trends in biotechnology.

[9]  R. Aslanyan,et al.  Callus formation in seven species of agarophyte marine algae , 1987 .

[10]  A. Bacic,et al.  A REVISION OF THE SYSTEMATICS OF THE NIZYMENIACEAE (GIGARTINALES, RHODOPHYTA) BASED ON POLYSACCHARIDES, ANATOMY, AND NUCLEOTIDE SEQUENCES 1 , 1995 .

[11]  G. Saunders GEL PURIFICATION OF RED ALGAL GENOMIC DNA: AN INEXPENSIVE AND RAPID METHOD FOR THE ISOLATION OF POLYMERASE CHAIN REACTION‐FRIENDLY DNA 1 , 1993 .

[12]  D. Kapraun,et al.  Nuclear genome characterization of the carrageenophyteAgardhiella subulata (Rhodophyta) , 1992, Journal of Applied Phycology.

[13]  R. Cerff,et al.  The marine red alga Chondrus crispus has a highly divergent β-tubulin gene with a characteristic 5′ intron: functional and evolutionary implications , 1995, Plant Molecular Biology.

[14]  A. Coleman,et al.  THE USE OF PLASTID DNA RESTRICTION ENDONUCLEASE PATTERNS IN DELINEATING RED ALGAL SPECIES AND POPULATIONS 1 , 1988 .

[15]  D. Morse,et al.  FRACTIONATION OF NUCLEAR, CHLOROPLAST, AND MITOCHONDRIAL DNA FROM POLYSIPHONIA BOLDII (RHODOPHYTA) USING A RAPID AND SIMPLE METHOD FOR THE SIMULTANEOUS ISOLATION OF RNA AND DNA 1 , 1991 .

[16]  L. J. Goff,et al.  PCR AMPLIFICATION OF NUCLEAR AND PLASTID GENES FROM ALGAL HERBARIUM SPECIMENS AND ALGAL SPORES 1 , 1993 .

[17]  C. Valentin,et al.  The GAPDH gene system of the red alga Chondrus crispus: promotor structures, intron/exon organization, genomic complexity and differential expression of genes , 1993, Plant Molecular Biology.

[18]  Cloning and characterization of chloroplast ribosomal protein-encoding genes, rpl16 and rps3, of the marine macro-algae, Gracilaria tenuistipitata. , 1990, Gene.

[19]  C. Valentin,et al.  The evolutionary origin of red algae as deduced from the nuclear genes encoding cytosolic and chloroplast glyceraldehyde-3-phosphate dehydrogenases from Chondrus crispus , 1994, Journal of Molecular Evolution.

[20]  A. Coleman,et al.  Red algal plasmids , 1990, Current Genetics.

[21]  R. Villemur The DNA sequence and structural organization of the GC2 plasmid from the red alga Gracilaria chilensis , 1990, Plant Molecular Biology.

[22]  B. Kloareg,et al.  Isolation of Protoplasts from Kappaphycus alvarezii var. tambalang (Rhodophyta) and Secretion of i-Carrageenan Fragments by Cultured Cells , 1993 .