Larrea tridentata extracts as an ecological strategy against Fusarium oxysporum radicis-lycopersici in tomato plants under greenhouse conditions.

The antifungal ability of extracts of Larrea tridentata in the inhibition of Fusarium oxysporum radicis-lycopersici in vitro and in vivo was evaluated. Once the Fusarium strain was characterized, the methodology of poisoned culture medium was used to determine the inhibition of mycelial growth at various concentrations (0-2000 ppm) and solvents (dichloromethane, methanol, ethanol and water). In the in vivo assay, ten treatments were evaluated: 2000 and 3000 ppm for DCM and EtOH, 3000 and 6000 ppm for MeOH and H2O and two controls; one plant as experimental unit and ten replicates. Plant height, leaf number, chlorophyll, severity, as well as incidence and survival were determined in tomato plants inoculated with the fungus and different extracts of L. tridentata. The extracts with a higher percentage of inhibition and vegetative development are dichloromethane at 3000 ppm and methanol at 4000 ppm, both in vitro and in vivo. Extracts of Larrea tridentata can be

[1]  Ramona Pérez-Leal,et al.  Situación actual en el control de Fusarium spp. y evaluación de la actividad antifúngica de extractos vegetales , 2014 .

[2]  I. Maldonado-Mendoza,et al.  Native soil bacteria isolates in Mexico exhibit a promising antagonistic effect against Fusarium oxysporum f. sp. radicis‐lycopersici , 2013, Journal of basic microbiology.

[3]  M. Staniaszek,et al.  Fusarium Oxysporum F. Sp. Radicis-Lycopersici – the Cause of Fusarium Crown and Root Rot in Tomato Cultivation , 2013 .

[4]  Cristóbal N. Aguilar,et al.  Antibacterial activity of plant extracts obtained with alternative organics solvents against food-borne pathogen bacteria , 2012 .

[5]  Cristóbal N. Aguilar,et al.  Bioactive compounds (phytoestrogens) recovery from Larrea tridentata leaves by solvents extraction , 2012 .

[6]  H. Sror,et al.  Effect of neem and willow aqueous extracts on fusarium wilt disease in tomato seedlings: Induction of antioxidant defensive enzymes , 2011 .

[7]  G. Ligarreto,et al.  Relación entre nitrógeno foliar y el contenido de clorofila, en maíz asociado con pastos en el Piedemonte Llanero colombiano , 2010 .

[8]  Enrique Rico-García,et al.  Nitrogen determination on tomato (Lycopersicon esculentum Mill.) seedlings by color image analysis (RGB) , 2010 .

[9]  G. Gallegos-Morales,et al.  Bioeficacia de productos orgánicos, biológicos y químicos contra Alternaria dauci Kühn y su efecto en el cultivo de zanahoria , 2006 .

[10]  T. Arie,et al.  PCR-based differentiation of Fusarium oxysporum ff. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici , 2006, Journal of General Plant Pathology.

[11]  A. Andrade-Cetto,et al.  Larrea tridentata (Creosote bush), an abundant plant of Mexican and US-American deserts and its metabolite nordihydroguaiaretic acid. , 2005, Journal of ethnopharmacology.

[12]  G. R. Townsend,et al.  Methods for estimating losses caused by diseases in fungicidal experiments , 1943 .

[13]  M. A. Gutiérrez-Coronado,et al.  BIOACTIVIDAD IN VITRO DE EXTRACTOS DE GOBERNADORA (LARREA TRIDENTATA) SOBRE LA INHIBICIÓN DE HONGOS POSCOSECHA: ALTERNARIA TENUISSIMA, ASPERGILLUS NIGER, PENICILLIUM POLONICUM Y RHIZOPUS ORYZAE , 2015 .

[14]  S. Moreno-Limón,et al.  Efecto antifúngico de extractos de gobernadora (Larrea tridentata L.) sobre la inhibición in vitro de Aspergillus flavus y Penicillium sp. , 2011 .

[15]  Cristóbal N. Aguilar,et al.  Antibacterial activity of crude methanolic extract and fractions obtained from Larrea tridentata leaves , 2011 .

[16]  A. Eskandari,et al.  Comparing Neem extract with chemical control on Fusarium oxysporum and Meloidogyne incognita complex of tomato , 2011 .

[17]  Cristóbal N. Aguilar,et al.  Biological efficiency of polyphenolic extracts from pecan nuts shell (Carya Illinoensis), pomegranate husk (Punica granatum) and creosote bush leaves (Larrea tridentata Cov.) against plant pathogenic fungi , 2010 .

[18]  Diana Jasso-Cantú,et al.  Actividad Biológica in vitro de Extractos de Flourensia cernua D.C. en Patógenos de Postcosecha: Alternaria alternata (Fr.:Fr.) Keissl., Colletotrichum gloeosporioides (Penz.) Penz. y Sacc. y Penicillium digitatum (Pers.:Fr.) Sacc. , 2007 .

[19]  E. Padrón-Corral,et al.  Inhibición del Crecimiento Micelial de Fusarium oxysporum Schlechtend. f. sp. lycopersici (Sacc.) Snyder y Hansen, Rhizoctonia solani Kühn y Verticilllium dahliae Kleb. Mediante Extractos Vegetales Acuosos , 2005 .

[20]  A. J. M. Quilambaqui Aislamiento e Identificación de Especies de Fusarium spp Asociadas al Declinamiento del Espárrago (Asparagus officinalis L.) en Cinco Municipios de Guanajuato, México , 2005 .

[21]  E. Mejía,et al.  Hospedantes asintomáticos de Fusarium oxysporum Schlechtend. f. sp. radicis-lycopersici W.R. Jarvis y Shoemaker en Sinaloa, México , 2004 .

[22]  Ricardo Hugo Lira Saldívar Estado Actual del Conocimiento sobre las Propiedades Biocidas de la Gobernadora [Larrea tridentata (D.C.) Coville] , 2003 .

[23]  F. Castillo,et al.  Evaluation of Resin Content and the Antifungal Effect of Larrea tridentata (Sesse and Moc. Ex D.C.) Coville Extracts from Two Mexican Deserts Against Pythium sp. Pringsh , 2003 .

[24]  E. Mejía,et al.  Frecuencia de Campos Infestados con Fusarium oxysporum f. sp. radicis-lycopersici en Sinaloa, México, y su Control , 2002 .

[25]  P. Kaufmann,et al.  Two genetically distinct populations of Fusarium oxysporum f.sp. lycopersici race 3 in the United States. , 1996 .