Combination of flow injection with capillary electrophoresis. Part I. The basic system

Abstract A combined flow injection (FI)-capillary electrophoresis (CE) system was developed using conventional FI and CE equipment. A flow-through reservoir equipped with a conical inlet was used as an interface to connect the FI system to the capillary inlet. A grounded electrode was inserted in the reservoir. Split-sampling was achieved in the electrokinetic mode when an injected sample zone in the microliter range was transported into the conical inlet by a carrier solution which also served as the separation buffer. Using benzoic acid as a model the combined system demonstrated significantly improved precision in peak height, peak area, and migration time as compared to conventional manual electrokinetic sample introduction while achieving similar sensitivity and column efficiency. Using a test sample containing magnolol and benzoic acid, a series of samples was injected continuously without current interruption, achieving sample throughputs at least three times higher than conventional CE sample introduction.

[1]  Professor Dr. Reinhard Kuhn,et al.  Capillary Electrophoresis: Principles and Practice , 1993, Springer Laboratory.

[2]  Purnendu K. Dasgupta,et al.  Flow-injection analysis in the capillary format using electroosmotic pumping , 1992 .

[3]  Sam F. Y. Li.  Capillary Electrophoresis: Principles, Practice and Applications , 1992 .

[4]  James P. Landers,et al.  Handbook of Capillary Electrophoresis , 1993 .

[5]  J. Jorgenson,et al.  Automated instrumentation for comprehensive two-dimensional high-performance liquid chromatography/capillary zone electrophoresis , 1990 .

[6]  James W. Jorgenson,et al.  Zone electrophoresis in open-tubular glass capillaries , 1981 .

[7]  D. J. Harrison,et al.  Planar chips technology for miniaturization and integration of separation techniques into monitoring systems. Capillary electrophoresis on a chip , 1992 .

[8]  R. Zare,et al.  Split injector for capillary zone electrophoresis , 1991 .

[9]  Jaromir Růžička,et al.  Flow injection analysis , 1981 .

[10]  Z. Fang.  Flow Injection Separation and Preconcentration , 1993 .

[11]  T. Tsuda,et al.  Rotary-type injector for capillary zone electrophoresis , 1987 .

[12]  S. Lunte,et al.  On-line coupling of in vivo microdialysis sampling with capillary electrophoresis. , 1994, Analytical chemistry.

[13]  Purnendu K. Dasgupta,et al.  Electroosmotically pumped capillary flow-injection analysis. Valve-based injection systems and sample throughput , 1993 .

[14]  J. Michael Ramsey,et al.  Effects of injection schemes and column geometry on the performance of microchip electrophoresis devices , 1994 .

[15]  F. Everaerts,et al.  High-performance zone electrophoresis , 1979 .