Phtyochemical screening and comparative study of antimicrobial activity of Aloe vera various extracts

The phytochemical screening and comparative study of antimicrobial activity of Aloe veraextracts was carried out. The phytochemical screening revealed the following metabolites: saponins, alkaloids, glycosides, tannins, protein, and flavonoids. While cardiac glyocosides and steroids were absent. The antimicrobial activities of the gel in 10% DMSO, methanol and aqueous extracts of green rind and leaf pulp were carried against some pathogenic clinical isolates, namely, Bacillus subtilis, Staphylococcus aureus, Proteus mirabilis and Candida albicans. The DMSO extract of the gel and the methanol extract of the green rind showed greater potency against tested microorganisms in the order B. Subtilis> S. aureus> C. albicans and S. aureus>C. albicans>B. subtilis>P. mirabilis respectively with different zones of inhibition ranging from 18 to 35 mm gel, 15 to 18 mm for leaf pulp methanol, 20 to 32 mm green rind methanol, also 12 to 14 mm and 14 to 23 mm for leaf pulp aqueous and green rind aqueous respectively. The minimum inhibitory concentration (MIC) of the extracts against organisms ranged from 6.25 to 25 mg/ml while the minimum bactericidal concentration (MBC) was within the range of 12.5 to 50 mg/ml. The study revealed that the green rind methanol extract and gel have greater medicinal potential against B. subtilis.   Key words: Antimicrobial, gel and green rind extract, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and phytochemical screening, Aloe vera.

[1]  R. Lawrence,et al.  Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera , 2009, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[2]  E. Igbinosa,et al.  Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn) , 2009 .

[3]  M. Haque,et al.  In vitro antibacterial screening and toxicity study of some different medicinal plants. , 2009 .

[4]  S. Alemdar,et al.  Investigation of in vitro antimicrobial activity of Aloe vera juice. , 2009 .

[5]  S. Arunkumar,et al.  Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens. , 2009 .

[6]  Josias H. Hamman,et al.  Composition and Applications of Aloe vera Leaf Gel , 2008, Molecules.

[7]  C. Ramachandra,et al.  Processing of Aloe Vera Leaf Gel: A Review , 2008 .

[8]  Lajbar Khan,et al.  Profile of heavy metals in selected medicinal plants. , 2008 .

[9]  K. Nahar,et al.  Plant characteristics, growth and leaf yield of Aloe vera as affected by organic manure in pot culture. , 2008 .

[10]  B. Freeman,et al.  An Overview of Plant Defenses against Pathogens and Herbivores , 2008 .

[11]  I. Tizard,et al.  Analytical methodology: the gel-analysis of aloe pulp and its derivatives , 2004 .

[12]  J. Spencer,et al.  Public health microbiology: methods and protocols. , 2004 .

[13]  W. Fang,et al.  Medicinal Chemistry Of Bioactive Natural Products , 2003 .

[14]  M. Chung,et al.  A review on the relationship between aloe vera components and their biologic effects , 2003 .

[15]  A C Dweck,et al.  Aloe vera leaf gel: a review update. , 1999, Journal of ethnopharmacology.

[16]  Tomoaki Inoue,et al.  Investigation of In Vitro Anaphylaxis Test. , 1998 .

[17]  J. van Staden,et al.  Antibacterial activity of South African plants used for medicinal purposes. , 1997, Journal of ethnopharmacology.

[18]  M. Moo-young,et al.  Antimicrobial activity of bark extracts of Bridelia ferruginea (Euphorbiaceae). , 1994, Journal of ethnopharmacology.

[19]  M. Cheesbrough.  Medical Laboratory Manual for Tropical Countries , 1991 .

[20]  J. Harborne.  Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis , 1973 .