Efficiency of Nannochloropsis oculata and Bacillus polymyxa symbiotic composite at ammonium and phosphate removal from synthetic wastewater

ABSTRACT Many issues, such as, DO accumulation, N2 fixation obstacle, and carbon dioxide diffusion, hamper the application of microalgae-alginate immobilization in wastewater treatment. The objective of this study was to evaluate the effect of the microalgae Nannochloropsis oculata immobilized with the bacterium Bacillus polymyxa in alginate on ammonium and phosphate removal from synthetic wastewater. Results show that the co-immobilized Bacillus-Nannochloropsis can exploit ammonium and phosphate from wastewater more effectively than the immobilized Nannochloropsis, and immobilized Bacillus alone. A significantly higher ammonium and phosphate removal efficiency was found in co-immobilized Bacillus-Nannochloropsis (59.85%, 90.44%) than of that in immobilized Nannochloropsis (49.56%, 77.36%), and Bacillus immobilized (31.46%, 29.66%) alone. Additionally, the most effective co-immobilization mixture ratio for wastewater treatment was found to contain equal suspension (108 cell/ml) volume of the Nannochloropsis and Bacillus. Nannochloris and Bacillus can coexist harmoniously with the symbiotic and synergistic relationship, and the Nannochloropsis oculata- Bacillus polymyxa combination can be useful as a potential method to develop novel wastewater treatment.

[1]  P. Altimari,et al.  Effect of Ca2+ concentration on Scenedesmus sp. growth in heterotrophic and photoautotrophic cultivation. , 2018, New biotechnology.

[2]  M. A. Moran,et al.  Interaction and signalling between a cosmopolitan phytoplankton and associated bacteria , 2015, Nature.

[3]  P. Altimari,et al.  Integrated biomass production and biodegradation of olive mill wastewater by cultivation of Scenedesmus sp. , 2015 .

[4]  Sanjoy Banerjee,et al.  Effects of different salinities and pH on the growth and proximate composition of Nannochloropsis sp. and Tetraselmis sp. isolated from South China Sea cultured under control and natural condition , 2014 .

[5]  H. Oh,et al.  Role of Rhizobium, a plant growth promoting bacterium, in enhancing algal biomass through mutualistic interaction , 2014 .

[6]  Paul Chen,et al.  Effect of wastewater-borne bacteria on algal growth and nutrients removal in wastewater-based algae cultivation system. , 2014, Bioresource technology.

[7]  P. Altimari,et al.  Mechanistic modelling of copper biosorption by wild type and engineered Saccharomyces cerevisiae biomasses , 2014 .

[8]  P. Altimari,et al.  Mixotrophic growth of Chlorella vulgaris and Nannochloropsis oculata: interaction between glucose and nitrate , 2014 .

[9]  Xuefei Zhou,et al.  The effect of bacterial contamination on the heterotrophic cultivation of Chlorella pyrenoidosa in wastewater from the production of soybean products. , 2012, Water research.

[10]  A. A. Al-Homaidan,et al.  Microalgae and wastewater treatment. , 2012, Saudi journal of biological sciences.

[11]  Guangce Wang,et al.  Immobilization of Chlorella sorokiniana GXNN 01 in alginate for removal of N and P from synthetic wastewater. , 2012, Bioresource technology.

[12]  Paul Chen,et al.  Local bioprospecting for high-lipid producing microalgal strains to be grown on concentrated municipal wastewater for biofuel production. , 2011, Bioresource technology.

[13]  R. Kolter,et al.  The Jekyll-and-Hyde chemistry of Phaeobacter gallaeciensis. , 2011, Nature chemistry.

[14]  Y. Bashan,et al.  EFFICIENCY OF GROWTH AND NUTRIENT UPTAKE FROM WASTEWATER BY HETEROTROPHIC, AUTOTROPHIC, AND MIXOTROPHIC CULTIVATION OF CHLORELLA VULGARIS IMMOBILIZED WITH AZOSPIRILLUM BRASILENSE 1 , 2010 .

[15]  Y. Bashan,et al.  Immobilized microalgae for removing pollutants: review of practical aspects. , 2010, Bioresource technology.

[16]  G. Pinto,et al.  Allelopathy and competition between Chlorella vulgaris and Pseudokirchneriella subcapitata: Experiments and mathematical model , 2007 .

[17]  Martin J. Warren,et al.  Algae acquire vitamin B12 through a symbiotic relationship with bacteria , 2005, Nature.

[18]  A. Reverberi,et al.  Copper adsorption on calcium alginate beads: equilibrium pH-related models , 2002 .

[19]  Y. Bashan,et al.  Increased Growth of the Microalga Chlorella vulgariswhen Coimmobilized and Cocultured in Alginate Beads with the Plant-Growth-Promoting Bacterium Azospirillum brasilense , 2000, Applied and Environmental Microbiology.

[20]  S. Baena,et al.  Efficiency of ammonia and phosphorus removal from a colombian agroindustrial wastewater by the microalgae Chlorella vulgaris and Scenedesmus dimorphus , 1997 .

[21]  J. Mouget,et al.  Algal growth enhancement by bacteria: Is consumption of photosynthetic oxygen involved? , 1995 .

[22]  Y. Bashan.  Alginate Beads as Synthetic Inoculant Carriers for Slow Release of Bacteria That Affect Plant Growth , 1986, Applied and environmental microbiology.

[23]  A. L. Dainty,et al.  Physiology of alginate-immobilized Chlorella , 1985 .

[24]  Jonathan J. Cole,et al.  INTERACTIONS BETWEEN BACTERIA AND ALGAE IN AQUATIC ECOSYSTEMS , 1982 .

[25]  H. Paerl,et al.  SIGNIFICANCE OF BACTERIAL‐ANABAENA (CYANOPHYCEAE) ASSOCIATIONS WITH RESPECT TO N2 FIXATION IN FRESHWATER 1, 2 , 1978 .

[26]  G. Rhee,et al.  COMPETITION BETWEEN AN ALGA AND AN AQUATIC BACTERIUM FOR PHOSPHATE1 , 1972 .

[27]  R. Safferman,et al.  Evaluation of natural products for algicidal properties. , 1962, Applied microbiology.

[28]  T. Mcmeekin,et al.  A NEW DIRECT NESSLERIZATION MICRO-KJELDAHL METHOD AND A MODIFICATION OF THE NESSLER-FOLIN REAGENT FOR AMMONIA , 1924 .

[29]  H. Oh,et al.  Algae-bacteria interactions: Evolution, ecology and emerging applications. , 2016, Biotechnology advances.

[30]  K. H. Goulding,et al.  Kinetics of phosphorus uptake by immobilizedChlorella , 2005, Biotechnology Letters.