Histological study of the effect of some irrigating solutions on bacterial endotoxin in dogs.

The aim of this study was to evaluate, histopathologically, the effectiveness of mechanical preparation of root canals using different irrigating solutions in dog teeth filled with LPS after pulpectomy. A total of 120 root canals of 6 mongrel dogs were filled with a solution of LPS after pulpectomy. The irrigating solutions used were saline, 1, 2.5, and 5% sodium hypochlorite, and 2% chlorhexidine. No irrigation was used in the control group. The animals were sacrificed after 60 days and the teeth were fixed and demineralized. Subsequently, serial 6-microm sections were stained with hematoxylin and eosin and Mallory's trichrome for histopathological analysis and Brown-Brenn for verification of bacterial contamination. Analysis showed that the inflammatory infiltrate was statistically less intense in the groups in which the root canals were irrigated with 5% sodium hypochlorite and 2% chlorhexidine. However, none of the irrigating solutions completely inactivated the harmful effects of LPS. Mechanical preparation associated with different irrigating solutions did not completely inactivate LPS.

[1]  M. Rossi,et al.  Effect of calcium hydroxide on bacterial endotoxin in vivo. , 2002, Journal of endodontics.

[2]  M. T. Filho,et al.  In vitro evaluation of the antimicrobial activity of a castor oil-based irrigant. , 2001, Journal of endodontics.

[3]  P. Eleazer,et al.  Detoxification of endotoxin by endodontic irrigants and calcium hydroxide. , 2001, Journal of endodontics.

[4]  R. Stevens,et al.  OR 1 Effect of chlorhexidine on IL-6 induction by LPS , 1999 .

[5]  L. A. Silva,et al.  In vivo antimicrobial activity of 2% chlorhexidine used as a root canal irrigating solution. , 1999, Journal of endodontics.

[6]  I. Heling,et al.  Antimicrobial effect of irrigant combinations within dentinal tubules. , 1998, International endodontic journal.

[7]  E. Berutti,et al.  Penetration ability of different irrigants into dentinal tubules. , 1997, Journal of endodontics.

[8]  M. Hirata,et al.  Lipopolysaccharides from Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans promote osteoclastic differentiation in vitro. , 1996, Archives of oral biology.

[9]  D. Lopatin,et al.  Anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence. , 1996, Endodontics & dental traumatology.

[10]  D. Pashley,et al.  Ability of bacterial endotoxin to diffuse through human dentin. , 1995, Journal of endodontics.

[11]  T. Kohsaka,et al.  Pulpal and periapical tissue reactions after experimental pulpal exposure in rats. , 1994, Journal of endodontics.

[12]  P. Toselli,et al.  Biochemical and histochemical analysis of the enzyme arylsulfatase in human lesions of endodontic origin. , 1993, Journal of endodontics.

[13]  C. Y. Wang,et al.  Characterization of bone-resorbing activity in human periapical lesions. , 1993, Journal of endodontics.

[14]  G. Sundqvist,et al.  Ecology of the root canal flora. , 1992, Journal of endodontics.

[15]  F. Weine,et al.  Tissue pH of developing periapical lesions in dogs. , 1983, Journal of endodontics.

[16]  J. Crawford,et al.  The detoxifying effect of varying concentrations of sodium hypochlorite on endotoxins. , 1982, Journal of endodontics.

[17]  B. Levy,et al.  [Antibody-producing cells in human periapical granulomas and cysts]. , 1981, Journal of endodontics.

[18]  M. Torabinejad,et al.  Radiographic and histologic evaluation of the effect of endotoxin on the periapical tissues of the cat. , 1981, Journal of endodontics.