Molecular cloning of the chicken progesterone receptor

To define the functional domains of the progesterone receptor required for gene regulation, complementary DNA (cDNA) clones encoding the chicken progesterone receptor have been isolated from a chicken oviduct lambda gt11 cDNA expression library. Positive clones expressed antigenic determinants that cross-reacted with six monospecific antibodies derived from two independent sources. A 36-amino acid peptide sequence obtained by microsequencing of purified progesterone receptor was encoded by nucleotide sequences in the longest cDNA clone. Analysis of the amino acid sequence of the progesterone receptor deduced from the cDNA clones revealed a cysteine-rich region that was homologous to a region found in the estrogen and glucocorticoid receptors and to the avian erythroblastosis virus gag-erb-A fusion protein. Northern blot analysis with chicken progesterone receptor cDNA's indicated the existence of at least three messenger RNA species. These messages were found only in oviduct and could be induced by estrogens.

[1]  P Argos,et al.  The chicken oestrogen receptor sequence: homology with v‐erbA and the human oestrogen and glucocorticoid receptors. , 1986, The EMBO journal.

[2]  P. Argos,et al.  Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A , 1986, Nature.

[3]  J. Shine,et al.  Sequence and expression of human estrogen receptor complementary DNA. , 1986, Science.

[4]  R. Evans,et al.  Primary structure and expression of a functional human glucocorticoid receptor cDNA , 1985, Nature.

[5]  P. Chambon,et al.  Cloning of the human estrogen receptor cDNA. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[6]  D. Melton,et al.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. , 1984, Nucleic acids research.

[7]  J. Claverie,et al.  Sequencing the erbA gene of avian erythroblastosis virus reveals a new type of oncogene. , 1984, Science.

[8]  E. Baulieu,et al.  Purification by affinity chromatography and immunological characterization of a 110kDa component of the chick oviduct progesterone receptor. , 1984, The Biochemical journal.

[9]  R. W. Davis,et al.  Yeast RNA polymerase II genes: isolation with antibody probes. , 1983, Science.

[10]  P. Chambon,et al.  Evidence for two structurally related progesterone receptors in chick oviduct cytosol , 1983, FEBS letters.

[11]  B. O’Malley,et al.  Covalent attachment of a progestational steroid to chick oviduct progesterone receptor by photoaffinity labelling , 1980, Nature.

[12]  R. Lerner,et al.  Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein. , 1977, The Journal of biological chemistry.

[13]  E. Baulieu,et al.  Mechanisms regulating the concentration and the conformation of progesterone receptor(s) in the uterus. , 1973, The Journal of biological chemistry.

[14]  B. O’Malley,et al.  Target tissue receptors for progesterone: the influence of estrogen treatment. , 1972, Endocrinology.

[15]  B. O’Malley,et al.  Progesterone-binding components of chick oviduct. IV. Characterization of purified subunits. , 1972, The Journal of biological chemistry.

[16]  E. Jensen,et al.  A two-step mechanism for the interaction of estradiol with rat uterus. , 1968, Proceedings of the National Academy of Sciences of the United States of America.

[17]  R. Evans,et al.  Domain structure of human glucocorticoid receptor and its relationship to the v-erb-A oncogene product , 1985, Nature.

[18]  W. Grody,et al.  Studies on the structure and function of the chicken progesterone receptor. , 1981, Recent progress in hormone research.