Comparing predicting models for the Escherichia coli inactivation by pulsed electric fields

Abstract Inactivation of Escherichia coli by pulsed electric field treatments (PEF) between 15 and 28 kV/cm in citrate–phosphate McIlvaine buffer (pH 7, 2 mS/cm) was studied. At all electric field strengths investigated the shape of the survival curves was concave upwards. A two-term exponential model for mixed cell populations, a model based on a Weibull distribution of resistances within the bacterial population, a sigmoidal equation also justified by the existence of a resistance distribution, and a purely empirical equation were used to fit the observed survival curves. The three last models were simpler than the first one and allowed to develop secondary models to estimate the influence of the electric field strength on the inactivation of E. coli. A validation study showed that the performance of models derived from the Weibull distribution and the empirical equation were better than the derived from the sigmoidal equation.

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