Capillary blood flow and cutaneous uptake of oxygen from the atmosphere

In 1 987 Baurngartl, using needle electrodes to measure P02 in skin, demonstrated that a minimum P02 value could be observed between the surface and the capillary bed. This indicated that there is an 02 flux through the epidermis supplying the upper layers of the skin. The recent development of a fluorescence-based 02-fluxoptode for measuring oxygen flux has meant that the phenomenon could be studied directly. More recently Stucker et. al. showed, using simultaneous measurement of oxygen flux, transcutaneous P02 and laser Doppler perfusion, that the epidermal oxygen uptake from the atmosphere is in balance with the blood-borne (haematogenic) oxygen supply. These measurements of oxygen flux and previous measurements of P02 profiles indicated that capillary blood flow in skin at normal skin temperature may not contribute to the oxygen supply of the superficial layers. In order to investigate this further, in the present study the transcutaneous hydrogen clearance technique was applied at various skin temperatures between 33°C and 40°C. Laser Doppler flowmetry (LDF), transcutaneous oxygen flux, and tsanscutaneous oxygen measurements were also carried at the same site. The inability to detect hydrogen at the skin surface at 33°C confirms the minimal contribution of capillary blood flow to the oxygen supply to superficial layers

[1]  D. Harrison,et al.  A multiwire hydrogen electrode for in vivo use. , 1989, Physics in medicine and biology.

[2]  J S Beck,et al.  Comparison of macro- and micro-lightguide spectrophotometric measurements of microvascular haemoglobin oxygenation in the tuberculin reaction in normal human skin. , 1994, Physiological measurement.

[3]  Paul Hartmann,et al.  Oxygen flux fluorescence lifetime imaging , 1997 .

[4]  J S Beck,et al.  Spectrophotometric measurements of haemoglobin saturation and concentration in skin during the tuberculin reaction in normal human subjects. , 1992, Clinical physics and physiological measurement : an official journal of the Hospital Physicists' Association, Deutsche Gesellschaft fur Medizinische Physik and the European Federation of Organisations for Medical Physics.

[5]  P. McCollum,et al.  Transcutaneous hydrogen clearance--a new non-invasive technique for assessing blood flow in human skin. , 1994, Physiological measurement.

[6]  D. Lübbers,et al.  FLOX—an oxygen-flux-measuring system using a phase-modulation method to evaluate the oxygen-dependent fluorescence lifetime , 1995 .

[7]  I. Silver,et al.  The oxygen dependence of cellular energy metabolism. , 1979, Archives of biochemistry and biophysics.

[8]  D W Lübbers,et al.  The transepidermal oxygen flux from the environment is in balance with the capillary oxygen supply. , 2000, The Journal of investigative dermatology.

[9]  D. Lübbers,et al.  FLIM of luminescent oxygen sensors: clinical applications and results , 1998 .

[10]  Otto S. Wolfbeis,et al.  A new sensing material for optical oxygen measurement, with the indicator embedded in an aqueous phase , 1986 .

[11]  Local hydrogen clearance as a method for the measurement of capillary blood flow. , 1989, Physics in medicine and biology.

[12]  P. McCollum,et al.  Amputation for peripheral vascular disease: The case for level selection , 1988, The British journal of surgery.