Fabrication of thermoset polyester microfluidic devices and embossing masters using rapid prototyped polydimethylsiloxane molds.

Plastics are increasingly being used for the fabrication of Lab-on-a-Chip devices due to the variety of beneficial material properties, affordable cost, and straightforward fabrication methods available from a range of different types of plastics. Rapid prototyping of polydimethylsiloxane (PDMS) devices has become a well-known process for the quick and easy fabrication of microfluidic devices in the research laboratory; however, PDMS is not always an appropriate material for every application. This paper describes the fabrication of thermoset polyester microfluidic devices and masters for hot embossing using replica molding techniques. Rapid prototyped PDMS molds are convienently used for the production of non-PDMS polymeric devices. The recessed features in the cast polyester can be bonded to a second polyester piece to form an enclosed microchannel. Thermoset polyester can withstand moderate amounts of pressure and elevated temperature; therefore, the cast polyester piece also can be used as a master for embossing polymethylmethacrylate (PMMA) microfluidic systems. Examples of enclosed polyester and PMMA microchannels are presented, and we discuss the electroosmotic properties of both types of channels, which are important for analytical applications such as capillary electrophoresis.

[1]  A. Manz,et al.  Micro total analysis systems. 1. Introduction, theory, and technology. , 2002, Analytical chemistry.

[2]  Lothar Bohn,et al.  Microstructure grooves with a width of less than 50 μm cut with ground hard metal micro end mills , 1999 .

[3]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[4]  L. Locascio,et al.  Measurement of electroosmotic flow in plastic imprinted microfluid devices and the effect of protein adsorption on flow rate. , 1999, Journal of chromatography. A.

[5]  S. Quake,et al.  A microfabricated fluorescence-activated cell sorter , 1999, Nature Biotechnology.

[6]  W. Xu,et al.  Fabrication of polyester microchannels and their applications to capillary electrophoresis. , 2001, Journal of chromatography. A.

[7]  R. Zare,et al.  Current-monitoring method for measuring the electroosmotic flow rate in capillary zone electrophoresis , 1988 .

[8]  C Gärtner,et al.  Polymer microfabrication methods for microfluidic analytical applications , 2000, Electrophoresis.

[9]  J. Henion,et al.  A polymeric microfluidic chip for CE/MS determination of small molecules. , 2001, Analytical chemistry.

[10]  R. McCormick,et al.  Microchannel electrophoretic separations of DNA in injection-molded plastic substrates. , 1997, Analytical chemistry.

[11]  S. Quake,et al.  Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.

[12]  S. Quake,et al.  From micro- to nanofabrication with soft materials. , 2000, Science.

[13]  G. Whitesides,et al.  Fabrication of microfluidic systems in poly(dimethylsiloxane) , 2000, Electrophoresis.

[14]  D. J. Harrison,et al.  Capillary electrophoresis and sample injection systems integrated on a planar glass chip , 1992 .

[15]  M. Madou.  Fundamentals of microfabrication , 1997 .

[16]  大房 健.  基礎講座 電気泳動(Electrophoresis) , 2005 .

[17]  D. J. Harrison,et al.  Planar chips technology for miniaturization and integration of separation techniques into monitoring systems. Capillary electrophoresis on a chip , 1992 .

[18]  Xu,et al.  Room-temperature imprinting method for plastic microchannel fabrication , 2000, Analytical chemistry.

[19]  H. Becker,et al.  Polymer microfluidic devices. , 2002, Talanta.

[20]  Shawn D. Llopis,et al.  Contact conductivity detection in poly(methyl methacrylate)-based microfluidic devices for analysis of mono- and polyanionic molecules. , 2002, Analytical chemistry.

[21]  A. B. Strong,et al.  Plastics: Materials and Processing , 1996 .

[22]  R. G. Christensen,et al.  Fabrication of plastic microfluid channels by imprinting methods. , 1997, Analytical chemistry.

[23]  G M Whitesides,et al.  Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping. , 2000, Analytical chemistry.