论文信息 - Hydrophilic/Hydrophobic Patterned Surfaces as Templates for DNA Arrays

Hydrophilic/Hydrophobic Patterned Surfaces as Templates for DNA Arrays

Addressable DNA arrays are created by photopatterning self-assembled monolayers to form hydrophilic and hydrophobic regions on a gold surface. The hydrophilic regions aid to contain small volumes of different DNA solutions placed on them using an automated pin-tool loading strategy. The method allows for efficient attachment, manipulation, and hybridization of pure DNA strands on the surface.

[1] A. Condon,et al. Demonstration of a word design strategy for DNA computing on surfaces. , 1997, Nucleic acids research.

[2] D. Shalon,et al. Gene expression micro-arrays: a new tool for genomic research. , 1998, Pathologie-biologie.

[3] S. P. Fodor,et al. Light-directed, spatially addressable parallel chemical synthesis. , 1991, Science.

[4] M. J. O'Donnell-Maloney,et al. Microfabrication and array technologies for DNA sequencing and diagnostics. , 1996, Genetic analysis : biomolecular engineering.

[5] R. Nuzzo,et al. ADSORPTION OF BIFUNCTIONAL ORGANIC DISULFIDES ON GOLD SURFACES , 1983 .

[6] Brian L. Frey,et al. Characterization of poly-L-lysine adsorption onto alkanethiol-modified gold surfaces with polarization-modulation Fourier transform infrared spectroscopy and surface plasmon resonance measurements , 1994 .

[7] J. Hodgson,et al. DNA chips: An array of possibilities , 1998, Nature Biotechnology.

[8] Qinghua Liu,et al. DNA Computing on Surfaces: Encoding Information at the Single Base Level , 1998, J. Comput. Biol..

[9] A. Blanchard,et al. High-density oligonucleotide arrays , 1996 .

[10] J. Hemminger,et al. Photopatterning of self-assembled alkanethiolate monolayers on gold. A simple monolayer photoresist utilizing aqueous chemistry , 1994 .