Capacity of Guiding Osseous Regeneration: Periosteum Versus Mesenchymal Stem Cells

The study was made on healthy common breed dogs on which, under general anesthesia, bone defects were made. For treatment were used scaffolds with hydroxyapatite-collagen support loaded with mesenchymal stem cells or the bone hole was covered with autologus periosteal flap. Evaluation of the healing process was made by clinical and, x-Ray exam. In guiding osseous regeneration in dog autologous periosteum has similar capacity, even better than autologous mesenchymal stem cells loaded on collagen impregnated with hydroxyapatite scaffolds.

[1]  M. Sabau,et al.  MESENCHYMAL STEM CELLS – A BIOLOGICAL ALTERNATIVE IN OSTEOARTICULAR RECONSTRUCTION IN DOGS , 2008 .

[2]  G. Tănăsie,et al.  Techniques for dog bone marrow stromal cells sampling, culturing, differentiation and loading scaffolds. , 2008 .

[3]  B. Rahn,et al.  Osteopromotion by a &bgr;-Tricalcium Phosphate/Bone Marrow Hybrid Implant for Use in Spine Surgery , 2006, Spine.

[4]  N. Baldini,et al.  In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination , 2005, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[5]  A. Uysal,et al.  Osteogenic Capacities of Periost Grafts, Periost Flaps and Prefabricated Periosteal Flaps: Experimental Study , 2005, The Journal of craniofacial surgery.

[6]  J. Lieberman,et al.  Healing of a critical-sized defect in the rat femur with use of a vascularized periosteal flap, a biodegradable matrix, and bone morphogenetic protein. , 2005, The Journal of bone and joint surgery. American volume.

[7]  I. Manjubala,et al.  Bone In-growth Induced by Biphasic Calcium Phosphate Ceramic in Femoral Defect of Dogs , 2005, Journal of biomaterials applications.

[8]  G. Daculsi,et al.  Calcium phosphate scaffold and bone marrow for bone reconstruction in irradiated area: a dog study. , 2005, Bone.

[9]  Rodolfo Quarto,et al.  Bone marrow stromal cells and their use in regenerating bone. , 2003, Novartis Foundation symposium.

[10]  G A Ilizarov,et al.  The tension-stress effect on the genesis and growth of tissues. Part I. The influence of stability of fixation and soft-tissue preservation. , 1989, Clinical orthopaedics and related research.

[11]  J. Lane.  Current Techniques in Small Animal Surgery , 1986 .

[12]  James D. Toombs,et al.  Current techniques in small animal surgery , 2014 .

[13]  F. W. Rhinelander.  The normal microcirculation of diaphyseal cortex and its response to fracture. , 1968, The Journal of bone and joint surgery. American volume.